Application of plants lactobacillus in fresh-keeping of freezing meat
A technology of Lactobacillus plantarum and Lactobacillus, applied in the direction of freezing/cooling preservation of meat/fish, bacteria, preservation of meat/fish with chemicals, etc., to achieve good antibacterial effect
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Embodiment 1
[0031] 1. Materials and methods
[0032] 1.1 Materials
[0033] 1.1.1 Strains
[0034] Lactic acid bacteria: isolated from the environment.
[0035] Indicator bacteria: Escherichia coli (Escherichia.coli) ACCC10503, Staphylococcus aureus (Staphylococcus aureus) ACCC10499, Bacillus cereus (Bacilluscereus) ACCC11108, Listeria monocytogenes (Listeria monocytogenes) ACCC11120, Pseudomonas fluorescens ( Pseudomomas fluorescens) ACCC10042 were all obtained from China Agricultural Microbiology Collection Center.
[0036] 1.1.2 Chilled meat raw materials
[0037] Pork tenderloin produced by Shuanghui Meat Group and cooled for 24 hours.
[0038] 1.1.3 Medium
[0039] 1.1.3.1 Lactic acid bacteria subculture medium (g / L)
[0040] MRS: peptone 10.0; beef extract 10.0, yeast extract 5.0, glucose 20.0; K2 HPO 4 2.0, triammonium citrate 2.0, sodium acetate 5.0, MgSO 4 .7H 2 O 0.58, MnSO 4 .4H 2 O 0.25, Tween 80 1ml, agar 20.0, pH 6.2~6.4, high temperature at 121℃
[0041] Autocl...
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