Freeze bag for storing liquid nitrogen refrigeration material

A liquid nitrogen freezing and cryopreservation technology, which is applied in bag making operations, gas/liquid distribution and storage, paper/cardboard containers, etc., can solve problems such as unsafe hidden dangers, damage, and easy-to-expand bags

Inactive Publication Date: 2008-07-23
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to aim at the bag body existing in various types of bags in the above-mentioned current liquid nitrogen cryopreservation process, which is easy to become hard, brittle and damaged at low temperature; poor permeability leads to slow freezing speed and residual The liquid-phase liquid nitrogen is easy to expand and crack the bag body after gasification; and there are defects such as inconvenient operation and unsafe hidden dangers. A kind of good low temperature resistance and high temperature resistance performan

Method used

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  • Freeze bag for storing liquid nitrogen refrigeration material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: (preparation of polyamide fiber cryopreservation bag)

[0025] A cryopreservation bag for storing liquid nitrogen freezing materials, the bag includes a bag body 1 with both sides and the lower end sealed and a bag mouth 2 with an open upper end, wherein the bag body 1 is ultra-low temperature resistant to -196°C and 120°C The 100-500 mesh nylon single (long) silk screen silk (net) of ℃ high temperature is material, and its two sides and the lower end are sewn and sealed; A keyhole opening 3 with a width of 1.5 cm and a width of 0.5 cm; the pocket opening 2 is turned outwards and downwards from the upper edge of the bag body 1, and is sewn together with the bag body 1 to form an annular tunnel with a keyhole opening 3 and an open upper end Formed after 4; Wherein, the keyhole opening 3 is positioned at the central axis of the outer side of the annular channel 4 of the bag body; a drawable rope 5 consistent with the material of the bag body 1 is set in the ...

Embodiment 2

[0034] Embodiment 2: (preparation of polyester freezer bag)

[0035] In this example, 100-400 mesh polyester single (long) silk screen silk (net) is used as the material; on either side of the bag body 0.8 cm from the top edge, a keyhole with a length of 2 cm and a width of 0.5 cm is processed with a keyhole machine Open the mouth; turn the upper edge of the bag body outward and down by 2.0 cm; choose polyester with a diameter of 0.4 cm and a length of 200 cm as the rope; use 1.0% SODOSIL for the semi-finished product of the frozen storage bag  The RAM 05 cleaning agent aqueous solution was ultrasonically soaked for 8 minutes and washed with water for 3 times; the rest of the process and steps were the same as in Example 1.

Embodiment 3

[0036] Embodiment 3: (the liquid nitrogen freezing of fresh Chinese cabbage blade)

[0037] Frozen material: choose intact fresh Chinese cabbage leaves;

[0038] Detection index: glucosinolate composition and content;

[0039] The purpose of freezing: to avoid the degradation of glucosinolates caused by tissue loss and damage;

[0040] Freeze storage bag: select the 200-mesh nylon freeze storage bag or polyester freeze storage bag of embodiment 1 or 2 for use;

[0041] Freezing steps and specific usage of freezer bags:

[0042] (1) Choose 32×23cm 2 freezer bags;

[0043] (2) Prepare labels (with lanyard), aluminum foil, liquid nitrogen (tank), freezing gloves, goggles, polyethylene foam board, a 500-mesh nylon freezing bag, insulation tank (less freezing material) or high-density Polyethylene foam insulation box (more frozen materials);

[0044] (3) Chinese cabbage leaves are wrapped with aluminum foil;

[0045] (4) After putting into the frozen storage bag, tie a label...

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Abstract

The invention discloses a cryopreservation bag used to store liquid nitrogen freezing materials, belonging to the technical field of cryopreservation. The bag comprises a bag body (1) and a bag mouth (2); the bag body (1) is made of nylon or polyester bolting silk which can resist the ultra-low temperature of minus 198 DEG C and the high temperature of 120 DEG C; a keyhole (3) is arranged at either side, 0.5 to 1.0 cm from top margin; the upper end margin of the bag body (1) is turned downward and is sewn into an annular channel (4) with a keyhole (3), forming the bag mouth (2); a drawing rope (5) is arranged in the annular channel (4); thus the bag is formed. The cryopreservation bag used to store liquid nitrogen freezing materials has the advantages that the bag can resist the ultra-low temperature as well as high temperature disinfection; the bag has strong permeability and excellent air permeability, improving the freezing speed of the sample and avoiding the problem of the expansion of the bag; the cryopreservation bag improves the operation efficiency and safety of liquid nitrogen freezing and can be used in scientific research or production field about materials needed to be stored with liquid nitrogen.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation, and relates to a cryopreservation container for storing liquid nitrogen freezing materials. Background technique [0002] In plant and animal scientific experimental research, some special materials often need to be stored and used by quick freezing. Because the liquid phase temperature of liquid nitrogen can reach -196°C, and it can absorb a large amount of heat during the rapid gasification process, so that the material to be frozen can be cooled rapidly in a very short period of time. Therefore, liquid nitrogen cryopreservation is the most commonly used in scientific research. A method for rapid freezing and preservation of special materials. [0003] During the rapid freezing process, in order to avoid contamination or mechanical mixing between different materials, it is necessary to take certain isolation measures. The isolation measures that are mainly u...

Claims

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Application Information

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IPC IPC(8): F17C3/04B31B49/04B31B70/00
Inventor 陈新娟孙高林杨悦俭阮美颖
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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