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Use of acid stable protease in animal feed

A technology of animal feed and protease, which is applied in the field of protease treatment of plant protein to achieve good acid-stability and improved performance

Inactive Publication Date: 2008-08-13
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, these proteases are not acid-stable and / or not homologous to the proteases described herein

Method used

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  • Use of acid stable protease in animal feed
  • Use of acid stable protease in animal feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 7

[0118] Examples of animal feed compositions are shown in Example 7.

[0119] Table B Value ranges for energy, protein and minerals in animal foods

[0120] Nutrients

poultry

piglet / pig / big

sow

fish

R.1

R.2

R.3

R.4

R.5

lowest-highest

lowest-highest

lowest-most

high

Metabolizable

energy,

MJ / kg

12.1-13.4

12.9-13.5

14-25

10-30

11-28

11-26

12-25

crude protein,

g / kg

124-280

120-240

300-480

50-800

75-700

100-600

110-500

120-490

Calcium, g / kg

8-40

5-9

10-15

0.1-200

0.5-150

1-100

4-50

usable

Phosphorus, g / kg

2.1-6.0

1.5-5.5

3-12

0.1-200

0.5-150

1-100

1-50

1-25

Methionine

Acid, g / kg

3.2-5.5

-

12-16...

Embodiment 1

[0133] Screening for acid-stable proteases

[0134] The stability of various proteases at pH 3 was analyzed with the aim of identifying proteases with the necessary stability to flow through the acidic stomach of monogastric animals.

[0135] By conventional chromatography, such as ion exchange chromatography, hydrophobic interaction chromatography and size exclusion chromatography (see for example Protein Purification, Principles, High Resolution Methods, and Applications, Jan-Christer Janson, edited by Lars Ryden, VCH Publishers, 1989 ) to purify protease.

[0136] Protease activity was determined as follows: protease was incubated with 1.67% Hammarsten casein at 25°C, pH 9.5 for 30 minutes, then TCA (trichloroacetic acid) was added to a final concentration of 2% (w / w), and the mixture was filtered to The precipitate was removed and the filtrate was analyzed for free primary amino groups (detected in a colorimetric assay based on OPA (o-phthalaldehyde) by measuring the ab...

Embodiment 2

[0143] Preparation, Identification and Comparative Study of Protease from Nocardiopsis

[0144] fermentation

[0145] Nocardiopsis alba was inoculated from tryptone yeast agar plates into flasks each containing 100 ml of HG-23 medium with the following composition: oat flour 45 g / l, yeast extract 2 g / l, disodium hydrogen phosphate 12 g / l 1. Potassium dihydrogen phosphate 6g / l, Pluronic PE 6100 0.2ml / l, dissolved in distilled water. The strain was fermented at 37°C for 9 days.

[0146] purification

[0147] The culture was centrifuged at 10000 x g for 30 minutes in a 1 liter beaker. The supernatants were pooled and further clarified by filtration through Seitz K-250 depth filter plates. The clarified filtrate was concentrated by ultrafiltration over a polyethersulfone cartridge (Filtron) with a molecular weight cut-off of 3 kDa. The concentrated enzyme was transferred to 50 mM H on a G25 Sephadex column (Amersham Pharmacia Biotech). 3 BO 3 , 5 mM 3,3′-dimethylgluta...

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Abstract

Acid-stable proteases of the subtilisin family, their use in animal feed, feed-additives and feed compositions

Description

[0001] This application is a divisional application of Chinese application 01804707.6. The parent application was filed on February 5, 2001 with the international application number PCT / EP01 / 01153, and entered the Chinese national phase on August 8, 2002. This divisional application adopts the same The title of the invention consistent with the parent application. technical field [0002] The present invention relates to the (in vivo) use of acid-stable proteases in animal feed and the (in vitro) use of such proteases for the treatment of vegetable proteins. [0003] Protein is an essential nutritional factor for animals and humans. Most livestock and many people get their essential protein from plant protein sources. Important vegetable protein sources are eg oilseed crops, pulses and cereals. [0004] For example, when soy flour is included in the feed of monogastric animals such as pigs and poultry, most of the soy flour solids cannot be digested. For example, the appare...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K1/16A23K1/175A23J3/16A23J3/34A23K10/30A23K20/189A23K50/30A23K50/75A23K50/80C12N9/52C12N9/56C12P21/00
CPCA23J3/16A23J3/346C12N9/52A23K10/30A23K20/189A23K50/75A23K50/30A23K50/80Y02A40/818
Inventor 彼得·R·奥斯特加尔德卡斯滕·肖霍姆
Owner DSM IP ASSETS BV
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