In-situ combination detection method for miRNA expression and cell apoptosis

A combined detection and RNA probe technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as limited information and difficult detection, and achieve the effect of filling the application gap and good in-situ detection effect
CN101240345AInactive Publication Date: 2008-08-13GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
Publication Date
2008-08-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for in-situ joint detection of the expression of miRNA and fluorescence in-situ hybridization detection of apoptosis. The method comprises: synthesizing miRNA specific probe modified by LNA, labeling the probe with digoxin, purifying, biotinzing mouse anti-digoxin and constructing affinity-fluorescence labeling system and other basic steps. The invention can in-situ joint detect the expression of miRNA and apoptosis at the same time. The detection method of the invention converts the expression condition of miRNA to visible fluorescence signal, getting a good in-situ detection effect and filling a gap in the application of miRNA in-suit fluorescence detection.
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Description

【Technical field】

[0001] The invention relates to the improvement of the miRNA in situ detection method. In particular, it is a fluorescent in situ hybridization detection method for in situ combined detection of miRNA expression and cell apoptosis. 【Background technique】

[0002] In recent years, studies have found that a new class of microRNA (microRNA or miRNA) refers to some special small non-coding RNAs with a length of about 21-25nt. By combining with its 3'untranslated region, it can negatively regulate gene expression by promoting the degradation of target mRNA and / or inhibiting the translation process at the post-transcriptional level. It is speculated that nearly 30% of human gene expression is precisely regulated by miRNAs. There are more than 300 miRNAs that have been identified so far. According to the speculation of the miRNA database Sanger, the total number may exceed 1000, accounting for 3% of the human genome. Because miRNAs exist widely in organisms and...

Claims

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