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Method for simultaneous determination for taurine, epsilon-amino caproic acid and L-aspartic acid content of medicament

A technology of aspartic acid and aminocaproic acid, which is applied in the field of determination of drug content, can solve the problems of long measurement time, high measurement cost, and low efficiency, and achieve the effects of simple operation, low reagent consumption, and short analysis time

Inactive Publication Date: 2011-10-05
SICHUAN UNIV
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Problems solved by technology

[0006] Due to the different determination conditions, high-performance liquid chromatography can only determine the content of taurine, ε-aminocaproic acid and L-aspartic acid in drugs alone, which has the disadvantages of long determination time, low efficiency and determination cost. higher deficiency

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  • Method for simultaneous determination for taurine, epsilon-amino caproic acid and L-aspartic acid content of medicament
  • Method for simultaneous determination for taurine, epsilon-amino caproic acid and L-aspartic acid content of medicament
  • Method for simultaneous determination for taurine, epsilon-amino caproic acid and L-aspartic acid content of medicament

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0024] The method for simultaneously measuring taurine, ε-aminocaproic acid and L-aspartic acid content in medicine comprises the steps:

[0025] ① Prepare the sample solution to be tested, and dilute the drug sample to be tested 600 times with 0.10 mol / L HCl solution.

[0026] ② Derivatization reaction, using borate buffer as the reaction medium, adding the sample solution to be tested, naphthalene dicarbaldehyde solution, and potassium cyanide solution to enter the derivation reaction. The specific process is as follows: take 150 μL of borate buffer solution with a pH value of 9.2 and a concentration of 10 mmol / L in a 0.5 mL polyethylene tube, then add 10 μL of mixed standard solution or sample to be tested, and 50 μL of borate buffer solution with a concentration of 2.0 mmol / L Naphthalene dicarbaldehyde solution and 50 μL of 20 mmol / L potassium cyan...

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Abstract

The present invention discloses a method for determining content of taurine, epsilon aminocaproic acid and L-aspartic acid in medicine at the same time, which comprises steps of: preparing sample solution to be measured; adding sample solution to be measured, naphthalenedicarboxaldehyde solution and potassium cyanide solution to process derivative reaction with borate buffer solution as reaction medium; entering capillary electrophoretic system to separate and detect, obtaining electrophoretogram of sample solution to be measured; comparing to electrophoretogram of mixture standard solution of taurine, epsilon aminocaproic acid and L-aspartic acid, confirming content of taurine, epsilon aminocaproic acid and L-aspartic acid in sample solution to be measured. The present invention can determine content of taurine, epsilon aminocaproic acid and L-aspartic acid in medicine expediently and rapidly, and has merits of simple operation, short analysis time, a little wastage of reagent and provides an optional method for general analysis and quality control of medicine.

Description

technical field [0001] The invention relates to a method for determining the content of medicine. Background technique [0002] In pharmaceutical preparations, taurine (Tau, 2-aminohexanesulfonic acid), ε-aminocaproic acid (EACA) and L-aspartic acid (L-Asp) can be added to achieve different pharmacological functions. Taurine is a sulfhydryl-containing amino acid that can be obtained through the metabolism of sulfur-containing amino acids reacting with glycine and γ-aminobutyric acid. Many research results show that taurine, as a neuroinhibitory transmitter, antioxidant, membrane stabilizer, transmission regulator, and intracellular calcium flow regulator, not only has cytoprotective properties and physiological functions, but also has pharmacological functions. Many diseases such as: Alzheimers disease, cardiovascular disease, hypercholesterolemia, epilepsy, abnormal liver function, muscular degeneration, alcoholism and cystic fibrosis have been proved to be related to taur...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
Inventor 肖丹杨秀培霍峰袁红雁蔡明发毕伟文
Owner SICHUAN UNIV
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