Arterial vessel tissue engineering reactor simulating physiological pulsating flow surrounding

A technique of arterial blood vessel and tissue engineering, which is applied in special-purpose bioreactors/fermenters, biochemical instruments, tissue cell/virus culture devices, etc., can solve the problem of not being able to provide a near-physiological flow environment, without careful consideration of flow impedance, Compliant resistance, flow inertia simulation and other issues, to achieve the effect of great promotion and application prospects, great social and economic benefits, and advanced functions

Active Publication Date: 2008-08-20
BEIHANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned reactor basically uses a peristaltic pump or a peristaltic pump plus a stepping motor as the pulsation source, and because the pulse wave simulation optimization design of the fluid circuit is not done, the simulat

Method used

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  • Arterial vessel tissue engineering reactor simulating physiological pulsating flow surrounding
  • Arterial vessel tissue engineering reactor simulating physiological pulsating flow surrounding
  • Arterial vessel tissue engineering reactor simulating physiological pulsating flow surrounding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Connect the reactor components according to Figure 2, including liquid storage bottle, pulsation source, resistance regulator 1, compliance regulator 1, vascular tissue dynamic culture chamber, resistance regulator 2 and compliance regulator 2;

[0036] 2. Disinfection reactor, disinfection conditions: 130°C, 3 atmospheres, 1 hour;

[0037] 3. Install tissue engineering blood vessels in the vascular tissue dynamic culture chamber;

[0038] 4. Connect the reactor with the monitoring and control device;

[0039] 5. Prepare the medium according to the cultivation requirements, and inject the sterile medium into the storage bottle;

[0040] 6. Select the preset cylindrical permanent magnet linear DC motor drive curve in the industrial computer, set the pulse frequency to 70 times / min, and set the initial position between the motor gain and 1-5%; start the cylindrical Permanent magnet linear DC motor, start perfusion culture;

[0041] 7. Adjust the resistance regulator...

Embodiment 2

[0043] 1. Connect the reactor components according to Figure 2, including liquid storage bottle, pulsation source, resistance regulator 1, compliance regulator 1, vascular tissue dynamic culture chamber, resistance regulator 2 and compliance regulator 2;

[0044] 2. Disinfection reactor, disinfection conditions: 130°C, 3 atmospheres, 1 hour;

[0045] 3. Install tissue engineering blood vessels in the vascular tissue dynamic culture chamber;

[0046] 4. Connect the reactor with the monitoring and control device;

[0047] 5. Prepare the medium according to the cultivation requirements, and inject the sterile medium into the storage bottle;

[0048] 6. Select the preset cylindrical permanent magnet linear DC motor drive curve in the industrial computer, set the pulse frequency to 70 times / min, and set the initial position between the motor gain and 1-5%; start the cylindrical Permanent magnet linear DC motor, start perfusion culture;

[0049] 7. Adjust the resistance regulator...

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Abstract

The invention discloses a quasi-physiological pulsatile flow environment arterial blood vessel tissue engineering reactor; the reactor includes a liquid storage bottle, a pulsation source, a resistance regulator, a compliance regulator and a blood vessel tissue dynamic culture cavity and is controlled by an industrial control computer; the reactor overcomes the shortcomings that the commonly used arterial blood vessel tissue engineering reactor in the tissue engineering filed can not simulate the blood flow compliance of the arterial blood vessel, flow inertia, flow resistance and other resistance characteristics and can not provide the quasi blood pulsatile flow environment in the artery; the reactor has the following functions that: a. the reactor is used for culturing the tissue engineering arterial blood vessel; b. the reactor is used for simulating the pulsating frequency, pulse waveform, secondary wave, amplitude and time phase of the pulsatile flow of the different arterial blood vessel under the physiological state, simulating the hemodynamics environment of high vessel inner pressure and high shear stress of the arterial blood vessel under the hypertension and other pathological states and simulating the hemodynamics status of the arterial blood vessel during the low shear stress.

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more particularly relates to a vascular tissue engineering reactor. Background technique [0002] In the field of tissue engineering, tissue engineering bioreactor is an important technical means to study the influence of different environmental factors (physical and biochemical factors) on the three-dimensional functional culture of specific cells and tissues. Automation, online monitoring, etc.), making tissue engineering a key link in the process of entering standard industrial scale production and clinical application from the laboratory. Therefore, targeted research and development of advanced bioreactors for tissue engineering is of great significance to the development of tissue engineering (Martin, et al., 2004; Portner, R., et al., 2005). [0003] So far, autografts, allogeneic blood vessels, xenograft blood vessels, and artificially synthesized blood vessels have...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/42
CPCC12M21/08C12M41/48C12M27/10C12M29/10C12M29/12C12M35/04
Inventor 樊瑜波邹远文李晋川黄学进贡向辉
Owner BEIHANG UNIV
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