Hemolytic agent for measuring white blood cell in hemocyte

A hemolytic agent and white blood cell technology, which is applied in the field of medical reagents for blood cell detection, can solve the problems of low error and achieve the effect of low manufacturing cost

Active Publication Date: 2008-08-20
深圳市雷诺华科技实业有限公司
0 Cites 12 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0005] Among the diagnostic reagents for blood cell analyzers currently on the market, there are two types of hemolytic agents. One is that it contains potassium cyanide, which is not conducive to environmental protection, but the hemolysis effect is good, and the results of white blood cell count and classification and hemoglobin are stabl...
View more

Method used

As can be seen from the detection result of table 2, hemolytic agent of the present invention has reached original design purpose. And compared wi...
View more

Abstract

The invention relates to a hemolytic agent for determining leukocyte in blood cells. The technology used in the program is that: hemolytic agent per 1000mL contains: polyoxyethylene type nonionic surfactant 6-22g, fixative 1-6g, cosolvent 1-9g, buffer 11-16g and excess deionized water. The material is easy to obtain, and the producing cost is low, the deviation between the result and the absolute accuracy is not more than 9%. The invention achieves accuracy and stability of classification and counting of leukocyte and test of blood cells in the absence of potassuim cyanide.

Application Domain

Biological testing

Technology Topic

IonChemistry +9

Image

  • Hemolytic agent for measuring white blood cell in hemocyte
  • Hemolytic agent for measuring white blood cell in hemocyte
  • Hemolytic agent for measuring white blood cell in hemocyte

Examples

  • Experimental program(5)
  • Effect test(1)

Example Embodiment

[0036] Example 1
[0037] 1) Preparation of buffer solution [0.2mol/L phosphate buffer (pH 6.2)]: Weigh 8.82 g of disodium hydrogen phosphate·12 water, dissolve in deionized water and dilute to 1L, use sodium hydroxide and hydrochloric acid for The pH was adjusted so that the pH value of the buffer solution was 6.2, and a sodium chloride regulator was added to make the osmotic pressure of the buffer solution 150 mOsm/kg.
[0038] 2) a hemolytic agent for measuring leukocytes in blood cells, the preparation method thereof is as follows:
[0039] will C 12 H 25 -O-(CH 2 CH 2 O) 9 -H 16g and
[0040] Glutaraldehyde 1.0g and
[0041] Cetyltrimethylammonium chloride C 16 H 33 (CH 3 ) 3 ClN 3.1g and
[0042] Buffer 14g
[0043] Dilute to 1000ml with deionized water, and then the hemolytic agent for measuring leukocytes in blood cells is obtained.
[0044] The hemolyzing agent completed with this composition and proportion is used to test the blood sample in a blood cell analyzer. The blood sample is added to the blood cell analyzer, diluted (WBC256 times\RBC5056 times) with a diluent (the diluent adopts the diluent in the prior art), and then enters the detection channel of white blood cells and hemoglobin. The hemolytic agent is mixed, and the blood samples of normal healthy people, physiological anemia patients and bacterial infection patients are tested under the conditions of pH 7.1, osmotic pressure 356mOsm/kg, conductivity 7.5mS/cm, and solution temperature 30℃ , using conventional detection methods, the detection can be completed within 1 minute. The test results are shown in Table 2 and figure 1 , figure 2 and image 3.
[0045] Table 2 Test results
[0046] project
[0047] It can be seen from the test results in Table 2 that the hemolytic agent of the present invention has achieved the original design purpose. And by comparing with the classification and counting of microscopy and the international standard method of hemoglobin detection, the results are in good agreement.

Example Embodiment

[0048] Example 2
[0049] 1) Preparation of buffer solution [0.2mol/L phosphate buffer solution (pH 6.3)]: Weigh 27.34 g of sodium dihydrogen phosphate·2 water, dissolve in deionized water and dilute to 1L. The pH was adjusted with sodium hydroxide and hydrochloric acid to make the pH value of the buffer solution 6.3, and a sodium chloride regulator was added to make the osmotic pressure of the buffer solution 250mOsm/kg.
[0050] 2) a hemolytic agent for measuring leukocytes in blood cells, the preparation method thereof is as follows:
[0051] will C 12 H 25 -O-(CH 2 CH2 O) 9 -H 6.0g and
[0052] Formaldehyde 1.0g and
[0053] Cetyltrimethylammonium bromide C 16 H 33 (CH 3 ) 3 BrN 2.6g and
[0054] Buffer 11g
[0055] Dilute to 1000ml with deionized water, and then the hemolytic agent for measuring leukocytes in blood cells is obtained.

Example Embodiment

[0056] Example 3
[0057] 1) Preparation of buffer solution: [Borate buffer solution (pH 9.1)]: Weigh 54.64 g of sodium tetraborate with 10 crystal waters, and dilute to 1000 ml with deionized water. The pH was adjusted with sodium hydroxide and hydrochloric acid so that the pH value of the buffer solution was 9.1, and a sodium sulfate regulator was added to make the osmotic pressure of the buffer solution 550 mOsm/kg.
[0058] 2) a hemolytic agent for measuring leukocytes in blood cells, the preparation method thereof is as follows:
[0059] Will 22.0g and
[0060] Glutaraldehyde 6.0g and
[0061] Cetyltrimethylammonium chloride C 16 H 33 (CH 3 ) 3 BrN 9.0g and
[0062] Buffer 16.0g
[0063] Dilute to 1000ml with deionized water, and then the hemolytic agent for measuring leukocytes in blood cells is obtained.

PUM

PropertyMeasurementUnit
Conductivity7.5ms/cm

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

Similar technology patents

Lithium-ion power cell aqueous sizing agent and manufacturing method thereof

InactiveCN102386374AImprove cycle lifelow manufacturing cost
Owner:ZHEJIANG CHAOWEI CHUANGYUAN INDUSTRAIAL

Method for manufacturing casing with textile fiber grain

InactiveCN101121310AReduce process steps and timelow manufacturing cost
Owner:林雪霞

Magnetic drying apparatus

InactiveUS20080120865A1low manufacturing costlow cost
Owner:SMITH HOLLY M

Vacuum insulated panel core material and manufacturing method thereof

ActiveCN101793340Alow manufacturing costImprove thermal insulation
Owner:CHONGQING ZAISHENG TECH CORP

Classification and recommendation of technical efficacy words

  • low manufacturing cost

Exercise equipment

InactiveUS6942605B1low manufacturing costlow price of sale
Owner:SUKHOVITSKY FELIKS

Compliance partitioning in testing of integrated circuits

InactiveUS20080061808A1low manufacturing costgood impedance control
Owner:INNOCONNEX

Inkjet recording apparatus

InactiveUS20050237352A1low manufacturing costfine image quality
Owner:KONICA MINOLTA MEDICAL & GRAPHICS INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products