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Hemolytic agent for measuring white blood cell in hemocyte

A hemolytic agent and white blood cell technology, which is applied in the field of medical reagents for blood cell detection, can solve the problems of low error and achieve the effect of low manufacturing cost

Active Publication Date: 2008-08-20
深圳市雷诺华科技实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among the diagnostic reagents for blood cell analyzers currently on the market, there are two types of hemolytic agents. One is that it contains potassium cyanide, which is not conducive to environmental protection, but the hemolysis effect is good, and the results of white blood cell count and classification and hemoglobin are stable; the other is that it does not contain Potassium cyanide, but when the result of hemoglobin measurement is low, it is often necessary to adjust the instrument calibration coefficient to a high value to increase the error. When some reagents classify white blood cells, the value of monocytes is relatively high within the normal range, etc.

Method used

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  • Hemolytic agent for measuring white blood cell in hemocyte
  • Hemolytic agent for measuring white blood cell in hemocyte
  • Hemolytic agent for measuring white blood cell in hemocyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Preparation of buffer solution [0.2mol / L phosphate buffer solution (pH 6.2)]: Weigh 8.82g of disodium hydrogen phosphate 12 water, dissolve it in deionized water and set the volume to 1L, and dilute it with sodium hydroxide and hydrochloric acid. The pH is adjusted so that the pH of the buffer is 6.2, and a sodium chloride regulator is added to make the osmotic pressure of the buffer 150mOsm / kg.

[0038] 2) a hemolytic agent for measuring white blood cells in blood cells, the preparation method of which is as follows:

[0039] Will C 12 h 25 -O-(CH 2 CH 2 O) 9 -H 16g and

[0040] Glutaraldehyde 1.0g and

[0041] Hexadecyltrimethylammonium Chloride C 16 h 33 (CH 3 ) 3 ClN 3.1g and

[0042] Buffer 14g

[0043] Set the volume to 1000ml with deionized water to obtain the hemolytic agent for the determination of leukocytes in blood cells.

[0044] The blood sample is detected in the blood cell analyzer by using the hemolyzing agent completed by the components...

Embodiment 2

[0049] 1) Preparation of buffer solution [0.2mol / L phosphate buffer solution (pH 6.3)]: Weigh 27.34g of sodium dihydrogen phosphate·2 water, dissolve with deionized water and dilute to 1L. The pH is adjusted with sodium hydroxide and hydrochloric acid to make the pH of the buffer solution 6.3, and a sodium chloride regulator is added to make the osmotic pressure of the buffer solution 250 mOsm / kg.

[0050] 2) a hemolytic agent for measuring white blood cells in blood cells, the preparation method of which is as follows:

[0051] Will C 12 h 25 -O-(CH 2 CH2 O) 9 -H 6.0g and

[0052] Formaldehyde 1.0g and

[0053] Hexadecyltrimethylammonium bromide C 16 h 33 (CH 3 ) 3 BrN 2.6g and

[0054] Buffer 11g

[0055] Set the volume to 1000ml with deionized water to obtain the hemolytic agent for the determination of leukocytes in blood cells.

Embodiment 3

[0057] 1) Preparation of buffer: [Borate buffer (pH9.1)]: Weigh 54.64g of sodium tetraborate with 10 crystal waters and dilute to 1000ml with deionized water. Adjust the pH with sodium hydroxide and hydrochloric acid to make the pH of the buffer solution 9.1, and add sodium sulfate regulator to make the osmotic pressure of the buffer solution 550 mOsm / kg.

[0058] 2) a hemolytic agent for measuring white blood cells in blood cells, the preparation method of which is as follows:

[0059] Will 22.0g and

[0060] Glutaraldehyde 6.0g and

[0061] Hexadecyltrimethylammonium Chloride C 16 h 33 (CH 3 ) 3 BrN 9.0g and

[0062] Buffer 16.0g

[0063] Set the volume to 1000ml with deionized water to obtain the hemolytic agent for the determination of leukocytes in blood cells.

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Abstract

The invention relates to a hemolytic agent for determining leukocyte in blood cells. The technology used in the program is that: hemolytic agent per 1000mL contains: polyoxyethylene type nonionic surfactant 6-22g, fixative 1-6g, cosolvent 1-9g, buffer 11-16g and excess deionized water. The material is easy to obtain, and the producing cost is low, the deviation between the result and the absolute accuracy is not more than 9%. The invention achieves accuracy and stability of classification and counting of leukocyte and test of blood cells in the absence of potassuim cyanide.

Description

Technical field: [0001] The invention relates to the field of medical reagents, in particular to a medical reagent used for blood cell detection in a blood cell analyzer. Background technique: [0002] Human blood cells contain WBC (white blood cells), RBC (red blood cells) and platelets. WBC is divided into: granulocytes, monocytes and lymphocytes; granulocytes are further divided into neutrophils, eosinophils, and basophils. . Healthy people's blood is within a certain range of the above components. However, some diseases and physiological changes, such as infection, fever, and pregnancy, will change to a certain extent in quantity or volume. Therefore, detection of blood cells is of great significance for disease diagnosis and treatment. [0003] Whether the hematology analyzers used in China are imported or domestic, the impedance method is mostly used in the past. The principle of counting cells by impedance method is based on the data obtained by comparing the puls...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 王亚东范钦信李雅杰梁文波
Owner 深圳市雷诺华科技实业有限公司
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