Hemolytic agent for measuring white blood cell in hemocyte

[0036] Example 1

[0037] 1) Preparation of buffer solution [0.2mol/L phosphate buffer (pH 6.2)]: Weigh 8.82 g of disodium hydrogen phosphate·12 water, dissolve in deionized water and dilute to 1L, use sodium hydroxide and hydrochloric acid for The pH was adjusted so that the pH value of the buffer solution was 6.2, and a sodium chloride regulator was added to make the osmotic pressure of the buffer solution 150 mOsm/kg.

[0038] 2) a hemolytic agent for measuring leukocytes in blood cells, the preparation method thereof is as follows:

[0039] will C 12 H 25 -O-(CH 2 CH 2 O) 9 -H 16g and

[0040] Glutaraldehyde 1.0g and

[0041] Cetyltrimethylammonium chloride C 16 H 33 (CH 3 ) 3 ClN 3.1g and

[0042] Buffer 14g

[0043] Dilute to 1000ml with deionized water, and then the hemolytic agent for measuring leukocytes in blood cells is obtained.

[0044] The hemolyzing agent completed with this composition and proportion is used to test the blood sample in a blood cell analyzer. The blood sample is added to the blood cell analyzer, diluted (WBC256 times\RBC5056 times) with a diluent (the diluent adopts the diluent in the prior art), and then enters the detection channel of white blood cells and hemoglobin. The hemolytic agent is mixed, and the blood samples of normal healthy people, physiological anemia patients and bacterial infection patients are tested under the conditions of pH 7.1, osmotic pressure 356mOsm/kg, conductivity 7.5mS/cm, and solution temperature 30℃ , using conventional detection methods, the detection can be completed within 1 minute. The test results are shown in Table 2 and figure 1 , figure 2 and image 3.

[0045] Table 2 Test results

[0046] project

[0047] It can be seen from the test results in Table 2 that the hemolytic agent of the present invention has achieved the original design purpose. And by comparing with the classification and counting of microscopy and the international standard method of hemoglobin detection, the results are in good agreement.

[0048] Example 2

[0049] 1) Preparation of buffer solution [0.2mol/L phosphate buffer solution (pH 6.3)]: Weigh 27.34 g of sodium dihydrogen phosphate·2 water, dissolve in deionized water and dilute to 1L. The pH was adjusted with sodium hydroxide and hydrochloric acid to make the pH value of the buffer solution 6.3, and a sodium chloride regulator was added to make the osmotic pressure of the buffer solution 250mOsm/kg.

[0050] 2) a hemolytic agent for measuring leukocytes in blood cells, the preparation method thereof is as follows:

[0051] will C 12 H 25 -O-(CH 2 CH2 O) 9 -H 6.0g and

[0052] Formaldehyde 1.0g and

[0053] Cetyltrimethylammonium bromide C 16 H 33 (CH 3 ) 3 BrN 2.6g and

[0054] Buffer 11g

[0055] Dilute to 1000ml with deionized water, and then the hemolytic agent for measuring leukocytes in blood cells is obtained.

[0056] Example 3

[0057] 1) Preparation of buffer solution: [Borate buffer solution (pH 9.1)]: Weigh 54.64 g of sodium tetraborate with 10 crystal waters, and dilute to 1000 ml with deionized water. The pH was adjusted with sodium hydroxide and hydrochloric acid so that the pH value of the buffer solution was 9.1, and a sodium sulfate regulator was added to make the osmotic pressure of the buffer solution 550 mOsm/kg.

[0058] 2) a hemolytic agent for measuring leukocytes in blood cells, the preparation method thereof is as follows:

[0059] Will 22.0g and

[0060] Glutaraldehyde 6.0g and

[0061] Cetyltrimethylammonium chloride C 16 H 33 (CH 3 ) 3 BrN 9.0g and

[0062] Buffer 16.0g

[0063] Dilute to 1000ml with deionized water, and then the hemolytic agent for measuring leukocytes in blood cells is obtained.