Method for partially oxidizing sodium alginate

A technology for oxidizing sodium alginate and sodium alginate, which is applied in the field of biomedical materials, can solve the problems of excessive hydrophilicity, lack of cell recognition sites, and high price of sodium periodate, and achieves that the method is simple, easy to control, and easy to popularize. The effect of application, easy availability of raw materials

Inactive Publication Date: 2008-09-10
HAINAN UNIVERSITY
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AI-Extracted Technical Summary

Problems solved by technology

However, there are two disadvantages when alginate hydrogel is used in biomedical materials: (1) alginate degrades slowly in vivo, and the degradation product is relatively large; (2) too strong hydrophilicity hinders Adsorption of protein components while lacking cellular recognition sites
The modification methods mainly include physical blending mo...
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Abstract

The invention discloses a method for partially oxidizing sodium alginate. The steps of the method are as follows: 1) a sodium alginate solution is prepared; 2) a potassium permanganate solution is prepared; 3) the pH value of the sodium alginate solution is adjusted by an acid solution; 4) the potassium permanganate solution is added to the sodium alginate solution and stirred for reaction under conditions of room temperature and light prevention; the oxidizing reaction stops when the mixed solution is colorless; 5) the solution after reaction is added with sodium chloride and then added with ethanol so as to separate precipitation out of the filtrate, and coarse products are obtained after decompression and pumping filtration; 6) the coarse products are dissolved in water and dialyzed; 7) after the complete dialysis, dialyzate is pre-freezed and then freeze-dried, and finally partially oxidizing sodium alginate products are obtained. The method has the advantages of simple and easy control of process, easy collection of raw materials, low cost and convenient popularization and application; the partially oxidizing sodium alginate prepared can be used to degrade gel materials, drug slow release materials, biological carrier materials and tissue engineering materials, etc.

Technology Topic

ChemistryTissue engineering +13

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  • Method for partially oxidizing sodium alginate
  • Method for partially oxidizing sodium alginate

Examples

  • Experimental program(3)

Example Embodiment

[0024] Example one
[0025] Weigh 1 g of sodium alginate and add it to 100 ml of deionized water, and magnetically stir until the sodium alginate is completely dissolved to obtain a 1% (w/v) sodium alginate solution. Weigh 1.5878g of potassium permanganate in 100ml of distilled water, stir and boil. After the potassium permanganate is fully dissolved, let it stand in the dark for 6 days, and finally take the upper clear solution for calibration. The concentration of the potassium permanganate solution is 0.1227mol /L, keep in brown bottle for later use. Add 0.5 ml (1:5) concentration of sulfuric acid aqueous solution dropwise to the sodium alginate solution, adjust the pH value to 4, and fully stir the solution. Next, accurately measure 1.5ml of the above potassium permanganate solution and add it to the sodium alginate solution, stir the reaction at room temperature and avoid light, and stop the oxidation reaction when the solution becomes colorless. Add 0.30g of sodium chloride to the reacted solution, add 250ml of ethanol to precipitate the precipitate after fully dissolving, and vacuum filtration to obtain the crude product. The crude product is fully dissolved in 50ml of water and then put into a dialysis bag (cut offMw= After dialysis in 10000), the dialysate is placed in a low-temperature refrigerator for pre-freezing for 12 hours after the dialysate is dialyzed, and then freeze-dried to obtain a partially oxidized sodium alginate product. The degree of oxidation of the product was determined to be 11.2%.

Example Embodiment

[0026] Embodiment two
[0027] Weigh 1 g of sodium alginate and add it to 100 ml of deionized water, and magnetically stir until the sodium alginate is completely dissolved to obtain a 1% (w/v) sodium alginate solution. Weigh 2.3756g of potassium permanganate in 100ml of distilled water, stir and boil. After the potassium permanganate is fully dissolved, let it stand in the dark for 8 days, and finally take the upper clear solution for calibration. The concentration of the potassium permanganate solution is 0.1671mol. /L, keep in brown bottle for later use. Add 0.5 ml (1:5) concentration of sulfuric acid aqueous solution dropwise to the sodium alginate solution, adjust the pH value to 4, and fully stir the solution. Then accurately measure 1.0 ml of the above potassium permanganate solution and add it to the sodium alginate solution, stir and react in the dark at room temperature, and stop the oxidation reaction when the solution becomes colorless. Add 0.30g of sodium chloride to the reacted solution, add 250ml of ethanol to precipitate the precipitate after fully dissolving, and vacuum filtration to obtain the crude product. The crude product is fully dissolved in 50ml of water and then put into a dialysis bag (cut offMw= After dialysis in 10000), the dialysate is placed in a low-temperature refrigerator for pre-freezing for 12 hours after the dialysate is dialyzed, and then freeze-dried to obtain a partially oxidized sodium alginate product. The degree of oxidation of the product was determined to be 6.66%.

Example Embodiment

[0028] Embodiment three
[0029] Weigh 1 g of sodium alginate and add it to 100 ml of deionized water, and magnetically stir until the sodium alginate is completely dissolved to obtain a 1% (w/v) sodium alginate solution. Weigh 1.2683g of potassium permanganate in 100ml of distilled water, stir and boil. After the potassium permanganate is fully dissolved, let it stand in the dark for 4 days, and finally take the upper clear solution for calibration. The concentration of the potassium permanganate solution is 0.0848mol /L, keep in brown bottle for later use. Add 0.3 ml (1:5) concentration of sulfuric acid aqueous solution dropwise to the sodium alginate solution, adjust the pH value to 3.5, and fully stir the solution. Then accurately measure 0.5ml of the above potassium permanganate solution and add it to the sodium alginate solution, stir and react in the dark at room temperature, and stop the oxidation reaction when the solution becomes colorless. 0.30g of sodium chloride was added to the obtained filtrate, 250ml of ethanol was added to precipitate the precipitate after being fully dissolved, and the crude product was obtained by suction filtration under reduced pressure. The crude product was fully dissolved in 50ml of water and then filled into a dialysis bag (cut offMw=10000 After the dialysate is dialyzed, the dialysate is placed in a low-temperature refrigerator for pre-freezing for 12 hours and freeze-dried to obtain a partially oxidized sodium alginate product. The degree of oxidation of the product was determined to be 9.52%.

PUM

PropertyMeasurementUnit
Concentration0.1227mol/l
Concentration0.1671mol/l

Description & Claims & Application Information

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