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Method for strengthening white rot fungus to excrete manganese peroxidase

A technology of manganese peroxidase and white rot fungi is applied in the field of fermentation of white rot fungi, which can solve the problems of long cycle and low output of manganese peroxidase, and achieve the effect of shortening the fermentation cycle.

Inactive Publication Date: 2008-09-10
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, at present white rot fungi secrete (or ferment) the period of manganese peroxidase long (being 6 days), and manganese peroxidase output is low (inoculum size is 10 7 / 100mL cultured for 6 days, manganese peroxidase production is less than 228U / L)

Method used

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  • Method for strengthening white rot fungus to excrete manganese peroxidase
  • Method for strengthening white rot fungus to excrete manganese peroxidase
  • Method for strengthening white rot fungus to excrete manganese peroxidase

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specific Embodiment approach 1

[0008] Specific embodiment one: this embodiment strengthens white-rot fungus to secrete manganese peroxidase and carries out according to the following steps: one, the white-rot fungus that grows vigorously in the PDA solid medium is inoculated in sterile water, filters after fully shaking, and then The white rot fungal spores in the filtrate are inoculated into the liquid enhanced medium with foam carrier; 2. The liquid enhanced medium is sealed with a sterilized sealing film, and then cultured in an environment at 37±1°C and a rotational speed of 160r / min for 3 days , that is to realize the strengthening of white rot fungi to secrete manganese peroxidase; wherein the inoculation amount of white rot fungal spores in the strengthening medium of step 1 is 10 7 / 100mL; in step 1, 1L liquid enriched medium is composed of 10g glucose, 2g ammonium tartrate, 2.0g KH 2 PO 4 , 0.535g MgSO 4 , 0.1g CaCl 2 , 20mmol acetic acid-sodium acetate buffer solution, 0.035g MnSO 4 , 0.07g Na...

specific Embodiment approach 2

[0013] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the white-rot fungus in Step 1 is Phanerochaete chrysosporium. Other steps and parameters are the same as those in Embodiment 1.

[0014] The Phanerochaete chrysosporium (P. Chrysosporium for short) selected in this embodiment is a commonly used biological research material. "Transformation of the white-rot filamentous fungus—Phanerochaete chrysosporium mediated by Agrobacterium tumefaciens" ("Acta Microbiology" 2005, vol. 45, No. 5, pp. 784-787) and "Phanerochaete chrysosporium) on three kinds of phenolic acid substances" ("Journal of Safety and Environment" 2006, Volume 6, Issue 5, Page 8-10), Phanerochaete chrysosporium was selected as the biological material.

specific Embodiment approach 3

[0015] Specific embodiment three: the difference between this embodiment and specific embodiment one is: filter with double gauze in step one. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a method for strengthening secretion of manganese peroxidases by white rot fungus which relates to a fermentation method for the white rot fungus. The invention solves the problems in the prior art of long period in secretion (or fermentation) of manganese peroxidases by the white rot fungus and low output of the manganese peroxidases. The method comprises the following steps: firstly, the eugonic white rot fungus in a PDA solid medium are inoculated into sterile water, fully oscillated and filtered, and then white rot fungi spores in filtrate are inoculated into a liquid strengthening culture medium which is provided with a foam carrier; secondly, the liquid strengthening culture medium is sealed by a sterilizing sealing membrane and placed into the environment with the temperature of 37+-1 DEG C and the rotary speed of 160 rotates per minute for culture of 3 days, and then strengthening of secretion of the manganese peroxidases by the white rot fungus is realized. The method for strengthening secretion of the manganese peroxidases by the white rot fungus reaches a peak of enzyme activity in fermentation broth on the third day for culture of the white rot fungus and is 3 days in advance compared with the prior method for fermentation of the manganese peroxidases by the white rot fungus, and the fermentation period is half-shortened. The output of the manganese peroxidases of the method is higher than 900 units per liter and is approximately four times of that of the enzyme output of the prior method.

Description

technical field [0001] The invention relates to a fermentation method of white rot fungi. Background technique [0002] With the development of industrial and agricultural production, more and more refractory organic substances are discharged into the environment in various forms, which has caused great harm to the living environment of human beings and seriously threatened the safety of the earth's ecosystem. At present, the methods for treating dye wastewater are mainly divided into physical chemical methods and biological methods. Commonly used physical and chemical technologies mainly include adsorption decolorization technology, coagulation precipitation technology, chemical oxidation technology, ion exchange technology, ultrafiltration membrane decolorization technology, photocatalytic technology, thermal oxidation technology, high-voltage pulse electrolysis technology, etc. Physical and chemical methods have a relatively high removal rate of wastewater chroma, which ...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N1/14C12R1/645
Inventor 高大文梁红曾永刚
Owner NORTHEAST FORESTRY UNIVERSITY
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