Method for researching interaction between drug and blood albumen and special micro-fluidic chip thereof
A microfluidic chip, drug technology, applied in testing pharmaceutical preparations, biological testing, material inspection products, etc., can solve problems such as poor repeatability
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Embodiment 1
[0025] Heparin is a highly sulfated linear polysaccharide. Heparin has a variety of biological activities, such as anticoagulant, anti-inflammatory, antiviral, antithrombotic and antilipidemia. Heparin is mainly used clinically as an anticoagulant, and can also be used as an antithrombotic and antilipidemia. This example mainly studies the interaction between heparin and serum proteins. Rhodamine B was used as the reference substance. The glass chip used had microchannels with a width of 50 microns and a depth of 15 microns. Rhodamine B, human serum albumin (1.36-21.77μM), human serum albumin and different concentrations of heparin mixture (21.77μM human serum albumin and 125.49, 250.98, 376.50, 501.96, and 627.45μM heparin) were respectively Put into the sample cells of the first, second and third units. Apply voltage to the sample pool and waste liquid pool of the first unit, and maintain the field strength at 300V / cm. After 19s of sampling, apply voltage to the buffer p...
Embodiment 2
[0027] This example is similar to Example 1, and also studies the interaction between heparin and serum proteins. The difference is that:
[0028] 1. The size of the microchannels of the glass chip used is different, the width is 70 microns, and the depth is 20 microns.
[0029] 2. The injection field strength is 500V / cm, and the injection time is 11s.
[0030] 3. The separation field strength is 800V / cm.
[0031] The experimental phenomena observed in this example are consistent with those in Example 1, and the measured values of binding constants and binding sites are also consistent with those measured in Example 1. However, because a higher field strength than in Example 1 was used, the time of the entire measurement process was shortened to 11 minutes.
Embodiment 3
[0033]Porphyrins are clinically used as photosensitizers for photodynamic therapy of cancer. Studies have shown that serum albumin can be used as an endogenous carrier of porphyrin to achieve photodynamic therapy of cancer. In order to deepen the understanding of the mechanism of action, the interaction constant between porphyrin and serum albumin is undoubtedly a very important parameter. This example investigates the interaction between porphyrin and human serum albumin. The glass chip used had microchannels with a width of 60 microns and a depth of 18 microns. Except adopting sodium fluorescein as the reference substance and changing the injection time to 5s, other operations were similar to Example 1. Figure 4 , 5 Experimental results are given. From Figure 5 , it can be seen that with the increase of porphyrin concentration, the peak of serum albumin gradually decreased. This indicates a binding reaction between porphyrin and serum albumin. The obtained binding c...
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