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Method for processing fish collagen oligopeptide

A processing method and oligopeptide technology, which can be used in protein food processing, animal protein processing, pharmaceutical formulations, etc., can solve the problems of non-recyclable, high salt content, fishy smell residue, etc., to avoid waste of resources, enzyme The effect of short solution time and no toxic side effects

Active Publication Date: 2011-04-20
BEIJING SEMNL BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of enzyme engineering technology to produce fish collagen is an advanced method commonly used at home and abroad, but the current processing methods mainly have the following problems: 1. Activated carbon is used for decolorization in the production process, and the products produced have high ash content and cannot be recycled; 2. Hydrolysis In the process, industrial grade hydrochloric acid and sodium hydroxide are used to adjust the pH value, and the final quality of the processed product cannot meet the requirements of food grade: 3. The aqueous solution of the processed product is not colorless, and there is a fishy smell; 4. The salt content of the product high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Called 10 kg of fish scales that have been taken off ash, put in the reaction irrigation, add 5 times the temperature of ionic water to clean, stir for 15 minutes to discharge the water and impurities, and then the fish scales after cleaning and stirred. After stirring for 15 minutes, drain the water and impurities, feed at 8 % of the dry weight of the raw material, add pH = 6.0 to remove the ion water, stir well, heat up to 90 ° C for 10 minutes, cool down to 53 ° C, the pH of the solution is exactly the pH just happened It is equal to 5.6, add 10 grams of papaya proteinase for 3 hours, then heating to 90 ° C, 10 minutes insulation enzymes, and then use diatomic filter filtering. The diatomic soil is 200 meters. The filtered supernatant is put into the storage tank, and then the supernatant is decolorized, deodorized and desalted through macroporous adsorption resin, cation exchange resin and anion exchange resin in turn, and then eluted after adsorption. The eluent is ...

Embodiment 2

[0032]Called 560 kg of fish scales that have been taken off ash, put in the reaction irrigation, add 7 times the room temperature to the ionic water to clean, stir for 20 minutes to discharge the water and impurities. Water, stir for 20 minutes before discharge the water and impurities. Perform 12 % of the dry weight of the raw materials. Add pH = 7.0 to remove ion water, stir well, heat up to 90 ° C for 10 minutes, cool down to 60 ° C, solution solution, solution solution The pH is exactly equal to 6.5. Add 2.0 kg of alkaline proteinase for 3 hours, then heating to 90 ° C, 10 minutes of insulation, then use diatom soil filter filtering For 0.5MP, the filtered liquid is filtered into the storage tank, and then the resin, cationic exchange resin, and the anionic resin can be removed and dehydrated by large pores. 7.0's de -ion water, the flow velocity is 3BV / h, and the collagen low polypeptide solution attached to the adsorized multi -layer filter membrane with a pore diameter ...

Embodiment 3

[0034] Called 480 kg of fish scales that have been taken off ash, put in the reaction irrigation, add 8 times room temperature to the ionic water to clean, stir for 30 minutes to discharge the water and impurities. Water, stir for 30 minutes before discharge the water and impurities. Perform 15 % of the dry weight of the raw materials, add pH = 7.0 to remove the ion water, stir well, increase the temperature to 100 ° C, heat up for 20 minutes, cool down to 65 ° C, cool down to 65 ° C, cool down, cool down to 65 ° C. The pH of the solution is equal to 7.5, adding 1.2 kg of alkaline protease and animal protein composite hydrolytic enzyme, 3 hours of enzyme disinfection, then heating to 90 ° C, insulation for 10 minutes to destroy the enzyme, then use the diatom soil filter to filter, the diatom soil is the soil as the soil. At 250 mesh, the pressure during the filtration process is 0.6MP. The filtered liquid is filtered into the storage tank, and then the resin, cedic exchange re...

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PUM

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Abstract

The invention discloses a processing method of fish collagen oligopeptide, which comprises the following steps: delimed and degreased dry scales are selected as raw material, after being treated with cleaning, pretreatment, enzymolysis, filtration, decolorization, taste escaping, desalinization, aseptic filtration, concentration, and spray drying, fish collagen oligopeptide powder is obtained; according to dry basis, the fish collagen oligopeptide powder contains: protein content of no less than 95 percent, ash content of less than 0.5 percent, and average molecular weight of 700Dalton. The product processed by the method of the invention has the advantages of high quality, colorlessness, no bitter taste or stink, low salt content, no poisonous or side effect, and short enzymolysis time, the resin filling used during the adsorption process is renewable and can be repeatedly used for a plurality of times, and the problems of wasting of resources and high ash content of the product caused by using the disposable activated charcoal are avoided, thus being widely applied in the fields of food, health products, medicine and cosmetics, etc.

Description

technical field [0001] The present invention involves a processing method of a low peptide, which specializes in the processing method of a fish collagen -ray peptide. Background technique [0002] Collagen is widely existed in the connective tissue of animal skin, bones, tendons, membranes and other connective tissues, and is the main component of connective tissue. In the past, the production of collagen mainly used terrestrial animals, such as cattle, pigs, bones, etc. as raw materials. Although the collagen of fish has long been recognized by humans, such as turtle plate gum, yellow croaker glue, etc., it has not been used as an important industrial application material. This is mainly based on the products of marine fishing. The source is unstable and lack of scale. In recent years, due to the influence of many factors such as environmental and infected diseases, the safety of protein has been questioned, and the aquaculture industry and the aquatic processing industry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/04A23J3/34A23L1/305A61K38/39A61K8/65A23L33/18
Inventor 刘爱青姜涛董峭
Owner BEIJING SEMNL BIOTECHNOLOGY CO LTD
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