Method for hydrolyzing propolis flavonoid glycosideby by naringinase

A gum flavonoid glycoside and naringinase technology, which is applied in the field of propolis flavonoid glycoside hydrolysis by naringinase, to achieve the effects of expanding the application range, improving antioxidant performance, and increasing added value

Inactive Publication Date: 2008-11-19
ZHEJIANG UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the bioavailability of flavonoid glycosides in

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for hydrolyzing propolis flavonoid glycosideby by naringinase
  • Method for hydrolyzing propolis flavonoid glycosideby by naringinase
  • Method for hydrolyzing propolis flavonoid glycosideby by naringinase

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] Take 100 mg of propolis, dissolve it in 200 mL of distilled water, add β-glucosidase, xylanase, cellulase, pectinase, and α-amylase for compound enzyme pretreatment. The enzyme dosage is 10U / mg and 1500U / mL, 9U / mL, 50U / mL, 1.5U / mL, pretreated at pH 5.0, temperature 50℃ for 6 hours, add a certain amount of naringinase for conversion, control the enzyme concentration to 30U / mg, pH 4.5 , The temperature is 50℃, the enzymolysis time is 24 hours, and the solution is always uniform during the reaction. After enzymolysis, add absolute ethanol to make the final concentration reach 60% to inactivate the enzyme. The flavonoid glycoside rutin in the propolis obtained under the enzymatic hydrolysis conditions decreased from 198.4mg / 100g before enzymolysis to 36.3mg / 100g after enzymolysis, and the hydrolysis rate reached 81.7%. The aglycones myricetone and quercetin The content of cortexin and kaempferol have increased, increasing by 53.2%, 63.2% and 95.9% respective...

Example Embodiment

[0025] Example 2

[0026] Take 200 mg of propolis and dissolve it in 200 mL of distilled water. Perform complex enzyme pretreatment as in Example 1. Add a certain amount of naringinase for conversion. Control the enzyme concentration to 10U / mg, pH 4.0, temperature 35°C, enzymatic hydrolysis time 9 hours , Keep the solution uniform during the reaction. After enzymolysis, add absolute ethanol to make the final concentration reach 60% to inactivate the enzyme. The flavonoid glycoside rutin in the propolis obtained under the enzymatic hydrolysis conditions decreased from 198.4mg / 100g before enzymolysis to 109.7mg / 100g after enzymolysis, and the hydrolysis rate reached 44.7%. The aglycones myricetone and quercetin The content of cortexin and kaempferol increased by 1.8%, 30.2% and 39.3% respectively (see Table 3, image 3 ).

[0027] Table 3 Contents of 5 flavonoids in propolis before and after enzymolysis (mg / 100g)

[0028]

[0029] The POV of propolis enzymatic hydrolysate on the 9...

Example Embodiment

[0032] Example 3

[0033] Take 800 mg of propolis and dissolve it in 200 mL of distilled water. Perform complex enzyme pretreatment as in Example 1. Add a certain amount of naringinase for conversion, control the enzyme concentration to 5U / mg, pH 6.5, temperature 60°C, enzymatic hydrolysis time 6 hours , Keep the solution uniform during the reaction. After enzymolysis, add absolute ethanol to make the final concentration reach 60% to inactivate the enzyme. The flavonoid glycoside rutin in the product propolis obtained under the enzymatic hydrolysis conditions decreased from 198.4mg / 100g before enzymolysis to 136.8mg / 100g after enzymolysis, and the hydrolysis rate reached 31.0%. The content of cortexin and kaempferol have increased, increasing by 5.3%, 18.8% and 20.2% respectively (see Table 5, Figure 5 ).

[0034] Table 5 Contents of 5 flavonoids in propolis before and after enzymatic hydrolysis (mg / 100g)

[0035]

[0036] The POV of the propolis enzymatic hydrolysate on the 9t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Hydrolysis rateaaaaaaaaaa
Login to view more

Abstract

The invention provides a method for hydrolyzing propolis flavonoid glycoside by naringinase. The method is as follows: naringinase is adopted to carry out constant-temperature enzymolysis of propolis flavonoid glycoside; propolis flavonoid glycoside substance is hydrolyzed into flavonoid aglycone through enzymolysis; moreover, compared with raw materials, the antioxidation property of the product is reinforced. The content of flavonoid substances in the obtained enzymolysis products can be measured through high-efficiency liquid phase chromatogram, and the result shows that the content of flavonoid glycoside substances is reduced, while the content of flavonoid aglycone substances is obviously increased. The method realizes deep development and utilization of propolis products, and is propitious to increase the added value of bee products and expand the application range of bee products. Moreover, the made propolis enzymolysis products has remarkably increased antioxidation property, and can be better used in the fields of medicine, foodstuff and cosmetics, etc. as compared with raw material propolis.

Description

technical field [0001] The invention belongs to propolis processing technology, and relates to a method for hydrolyzing propolis flavonoid glycosides with naringinase. technical background [0002] Propolis (propolis) is a colloidal solid with an aromatic smell that bees collect resin from plant buds, bark and trunk cracks, and mix it with their mandibular gland secretions and beeswax. Propolis is called "black wax" in the ancient Arabic medical book "Medical Code", and it is also one of the main components of the honeycomb that was first recorded in "Shen Nong's Materia Medica". Propolis has various biological effects such as preventing wound infection, regulating immunity, anti-tumor, lowering blood pressure, lowering blood fat, lowering blood sugar and resisting pathogenic microorganisms. The chemical composition of propolis is complex, containing flavonoids, acids, alcohols, phenols, aldehydes, esters, ethers, alkenes, terpenes, steroids and various amino acids, fatty a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P17/06
Inventor 胡福良刘亚男李英华
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap