High density lipoprotein cholesterin fraction esterification rate determination method

A technique for high-density lipoprotein and a determination method is applied in the field of determination of the fractional esterification rate of lipoprotein cholesterol, which can solve the problems of inconvenient research and application, difficult sample storage and the like, and achieve the effects of simple determination and low cost.
CN101308122BInactive Publication Date: 2011-05-11BEIJING HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING HOSPITAL
Publication Date
2011-05-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a measuring method of high density lipoprotein cholesterol fraction esterification velocity, which comprises: a) adding a lecithin cholesterol acyltransferase activity inhibitor in a fresh whole blood sample; b) adding a precipitator to obtain a sample only containing high density lipoprotein; c) dividing the sample into two parts with one part added with a lecithin cholesterol acyltransferase activity restorer and the other part added with nothing, and the two parts are cultivated at a temperature of 37 DEG C for one hour; and d) utilizing the high efficiency liquid phase chromatography to respectively measure high density lipoprotein free cholesterol of the two samples and calculating the difference between the two parts, and obtaining the high density lipoprotein cholesterol fraction esterification velocity by dividing the difference value by the detected percentage of high density lipoprotein free cholesterol of the sample without adding lecithin cholesterol acyltransferase activity restorer. The method of the invention has advantages of simplicity, safety, low cost and storability of samples.
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Description

technical field

[0001] The invention belongs to the detection field, and more specifically, the invention belongs to a method for measuring the esterification rate of lipoprotein cholesterol fraction. Background technique

[0002] Lecithin cholesterol acyltransferase (LCAT) is the main enzyme of cholesterol esterification in the circulation. It transfers the fatty acyl group at the sn-2 position of lecithin to cholesterol to generate cholesterol and lysolecithin. LCAT binds to high-density lipoprotein (HDL) , so cholesterol esterification mainly occurs in HDL. LCAT plays an important role in HDL metabolism and cholesterol reverse transport (Glomset JA. The plasma lecithin: cholesterololacyltransferase reaction. J Lipid Res, 1968, 9: 155-167;, Sviridov D, Nestel PDynamics of reverse cholesterol transport: protection against atherosclerosis. Atherosclerosis, 2002, 161: 245-254.). There are many methods for studying LCAT activity, one of which is to measure the cholesterol es...

Claims

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