Process for double-aqueous-phase extracting and refining persimmon lycopene

A lycopene, two-phase technology, applied in extraction purification/separation, organic chemistry and other directions, can solve the problems of affecting the market competitiveness of lycopene, high production cost, poor product quality, etc. The effect of less pollution and simple method

Inactive Publication Date: 2008-11-26
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The products obtained by organic solvent extraction are of poor quality, low purity, odor and solvent residue
It has also been reported that supercritical CO 2 The extraction method is

Method used

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  • Process for double-aqueous-phase extracting and refining persimmon lycopene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step 1. Vacuum freeze-drying: sort and clean fresh persimmon fruits (seeds), take 160g and cut them into 5mm square and 10mm thick blocks, put them on a plate, and pre-freeze them at -20°C with a pre-freezing rate of 0.03 hours / mm , at a vacuum degree of 20 Pa, a heating plate at 25° C., sublimation drying and analysis drying at a cold trap of -35° C., to obtain 10 g of freeze-dried persimmon fruit (seed) grains.

[0030] Step 2, crude organic solvent extraction: crush 10 g of vacuum freeze-dried persimmon fruit (seeds), add 100 mL of acetone, perform solid-liquid extraction for 3 hours, and obtain 68 mL of crude lycopene extract after filtration.

[0031] Step 3, two-phase extraction and refining: take 68mL of the above crude lycopene extract, add 10g cellulose methyl ether, 10g glycerin, 50mL water, and then add 1g MgCl 2 , mix well, and separate the phases at 15°C for 3 hours.

[0032] Step 4, ethyl acetate stripping: take out 55mL of cellulose methyl ether phase ri...

Embodiment 2

[0034] Step 1. Vacuum freeze-drying: sort and clean fresh persimmon fruit (seeds), take 160g and cut into 5mm square, 15mm thick blocks, put them on a plate, and pre-freeze them at -25°C with a pre-freezing rate of 0.05 hours / mm , at a vacuum degree of 18Pa, a heating plate at 25°C, and a cold trap at -35°C for sublimation drying and analysis drying to obtain 10 g of freeze-dried persimmon fruit (seed) grains.

[0035] Step 2, crude organic solvent extraction: pulverize 10 g of vacuum freeze-dried persimmon fruit (seeds), add 150 mL of acetone, perform solid-liquid extraction for 3 hours, and obtain 102 mL of crude lycopene extract after filtration.

[0036] Step 3, two-phase extraction and refining: take 102mL of the above lycopene crude extract, add 15g of cellulose methyl ether, 12g of glycerin, 60mL of water, and then add 2g of MgCl 2 , mix well, and separate the phases at 30°C for 6 hours.

[0037] Step 4, ethyl acetate stripping: take out 75mL of cellulose methyl ether ...

Embodiment 3

[0040] Step 1. Vacuum freeze-drying: sort and clean fresh persimmon fruits (seeds), take 160g and cut them into 5mm square and 15mm thick blocks, put them on a plate, and pre-freeze them at -25°C with a pre-freezing rate of 0.04 hours / mm , at a vacuum degree of 20 Pa, a heating plate at 25° C., sublimation drying and analysis drying at a cold trap of -35° C., to obtain 10 g of freeze-dried persimmon fruit (seed) grains.

[0041] Step 2, crude organic solvent extraction: crush 10 g of vacuum freeze-dried persimmon fruit (seeds), add 180 mL of acetone, perform solid-liquid extraction for 3 hours, and obtain 76 mL of crude lycopene extract after filtration.

[0042] Step 3, two-phase extraction and refining: Take 76 mL of the crude lycopene extract, add 24 g of cellulose ether, 18 g of glycerin, 70 mL of water, and then add 3 g of NaCl, mix well, and separate phases at 25 ° C for 5 hours.

[0043] Step 4, butyl acetate stripping: Take out 65 mL of the cellulose ether phase rich i...

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Abstract

The invention relates to an aqueous two-phase extraction and refinement method for persimmon lycopene, which is technically characterized in that persimmons are washed; after rotten fruits are removed, the persimmons are diced, carry out vacuum freeze drying, and are crushed, added with acetone, and then carry out solid-liquid extraction; after filtering, lycopene crude extract is obtained. The crude extract is taken, added with cellulose ether and glycerin aqueous two-phase system, evenly mixed and divided into an upper phase and a lower phase. The phase of cellulose ether which is rich in lycopene is taken out, added with water, acetic ester and inorganic salt for evenly mixing. The phase of acetic ester which is rich in lycopene is taken out for reduced pressure distillation and solution concentration, and then the concentrated solution carries out vacuum drying to obtain lycopene solid. The invention has the advantages that the method increases the extraction yield of persimmon lycopene, obtains high-quality lycopene solid, which is economical, nontoxic, simple in operation and easy for industrial production.

Description

technical field [0001] The invention relates to a method for refining persimmon fruit (seeds) lycopene by two-phase extraction, and relates to a method of vacuum freeze-drying persimmon fruit (seeds), rough extraction with an organic solvent, and then refining persimmon fruit by two-phase extraction The (sub) lycopene approach. Background technique [0002] Lycopene (Lycopene) is a kind of carotenoid, which is red in color and got its name because it was first isolated from tomato. The latest research has found that lycopene has superior physiological functions. Lycopene is the carotenoid with the strongest antioxidant activity, and its rate constant for scavenging singlet oxygen is 100 times that of vitamin E, a commonly used antioxidant. Lycopene can effectively prevent prostate cancer, and its inhibitory effect on uterine cancer and lung cancer cells is significantly higher than that of β-carotene and α-carotene, and the content of lycopene in the human body is related ...

Claims

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Application Information

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IPC IPC(8): C07C11/21C07C7/10
Inventor 岳红郭云珠
Owner NORTHWESTERN POLYTECHNICAL UNIV
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