Method for extracting flavone from capillary wormwood

A technology for removing impurities from wormwood and wormwood, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc. Inactivation and other problems, to achieve the effect of high yield, reduced solubility, low extraction temperature

Inactive Publication Date: 2010-08-11
山西师范大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention solves the problems of low extraction rate and high extraction temperature in the existing method for extracting flavonoids from Capillaris sinensis, which aggravates the dissolution of non-target components (impurities), also easily causes extract deactivation and organic solvent residues, and lower production costs. Advanced problem, provide a kind of method for extracting flavonoids from Mian Yin Chen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A method for extracting flavonoids from Capillaris mirabilis, comprising the following steps: (1) removing impurities, screening, drying, and crushing Capillaria migraine into 40-mesh sieves; Extracting agent 20 times of the weight of capillary wormwood, then sealed, protected from light, leaching for 1.0h at a temperature of 40°C, and finally suction-filtered to obtain flavonoid extraction crude liquid, the extracting agent is made of cellulase with a concentration of 0.02%. solution and pectinase solution mixed with a volume ratio of 1: 1.5, the pH value of the extractant is 4.5; (3) the crude flavonoid extraction solution obtained in step (2) is concentrated in a vacuum and then added with water ethanol, so that the ethanol concentration in the flavonoid extract reaches 98%, through reaction, precipitation, filtration to remove macromolecular substances, obtain the flavone extract of preliminary purification; (4) the flavone extract obtained by step (3) is concentrate...

Embodiment 2

[0014] A method for extracting flavonoids from Capillaris mirabilis, comprising the following steps: (1) removing impurities, screening and drying the Capillaria migraine, crushing it into 50-mesh sieve; (2) adding the equivalent Extracting agent 30 times the weight of capillary wormwood, then sealed, protected from light, leaching at a temperature of 60°C for 2.0h, and finally suction-filtered to obtain flavonoid extraction crude liquid, the extracting agent is made of cellulase with a concentration of 0.02%. solution and pectinase solution mixed with a volume ratio of 1: 2.5, the pH value of the extractant is 5.5; (3) the crude flavonoid extraction solution obtained in step (2) is concentrated in a vacuum and then added with water ethanol, so that the ethanol concentration in the flavonoid extract reaches 85%, through reaction, precipitation, filtration to remove macromolecular substances, obtain the flavone extract of preliminary purification; (4) the flavone extract obtaine...

Embodiment 3

[0016] A method for extracting flavonoids from Capillaris mirabilis, comprising the following steps: (1) removing impurities, screening and drying the Capillaris miabilis, crushing it into a 45-mesh sieve; Extracting agent with 20-30 times the weight of capillary wormwood, then sealed, protected from light, leaching at a temperature of 50°C for 1.5h, and finally suction-filtered to obtain flavonoid extraction crude liquid, the extracting agent is made of fiber with a concentration of 0.02%. Sulfase solution and pectinase solution are mixed with a volume ratio of 1: 2.0 to form a compound enzyme solution, and the pH value of the extractant is 5.0; (3) after the flavonoid extraction crude liquid obtained in step (2) is concentrated in a vacuum Add dehydrated alcohol to make the ethanol concentration in the flavonoid extract reach 90%, react, precipitate and filter to remove macromolecular substances, and obtain a preliminary purified flavone extract; (4) cool the flavone extract ...

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PUM

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Abstract

The present invention relates to the field of Chinese traditional medicine abstraction, in particular to a method for abstracting flavone from capillary wormwood. The method is used for solving the problems of low extraction yield, high temperature, inactivation of extraction, residue of organic solvent, etc., existing in the prior flavone extracting method. The method comprises edulcoration, screen and dryness of capillary wormwood; extractant is put into a capillary wormwood; then lixiviation and pump filter are performed so that raw extracting solution is obtained. The extractant is obtained by mixing cellulolytic enzyme and pectinase; rarefied flavone extracting solution is obtained after absolute ethyl alcohol is put into the flavone extracting solution subject to vacuum concentration. Then the ethanol is subject to cryoconcentration and reclamation and the concentrated solution is dried at the same time. Powdered flavone extraction is obtained. Low extraction temperature in the present invention decreases solvency of impurity. The extraction is high in purity and the extraction yield is good; the obtained capillary wormwood flavone is pure natural product. Freeze drying prevents effective ingredient of the flavone from being destroyed; the biological activity is high; the end product has no organic solvent residue; the method is simple in technological operation, good inrepetitiveness, and high in productive rate. Therefore the method is suitable for industrialized production.

Description

technical field [0001] The invention relates to the field of extraction of traditional Chinese medicines, in particular to a method for extracting flavonoids from capillary wormwood. Background technique [0002] Capillaris is the dry aerial part of Artemisia scoparia Waldst. et kit. or Artemisia capillaries Thunb., a perennial herb of Compositae. It is distributed in most parts of our country, and is mainly produced in Shaanxi, Shanxi, Anhui and other places. It is commonly called "Mianyinchen" harvested in spring, and "Artemisia yinchen" harvested in autumn. Mianjunchen is one of the commonly used traditional Chinese medicines in clinical practice. It tastes bitter, pungent, and slightly cold. It returns to the spleen, stomach, and liver and gallbladder meridian. , infectious jaundice hepatitis embolism, containing flavonoids, chlorogenic acid and volatile oil and other active ingredients. Modern pharmacological research has found that flavonoids, as an important biologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61P9/12A61P3/06A61P7/02A61P35/00
Inventor 徐建国胡青平赵猛朱洪梅
Owner 山西师范大学
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