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Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration

A technology of inorganic phosphorus and kits, applied in the field of determination of inorganic phosphorus concentration, inorganic phosphorus diagnosis/measurement kits, can solve the problem of high results

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, jaundice, hemolysis, and fat-turbid serum have light absorption at 340nm, and a blank specimen must be prepared, otherwise the result will be high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] The inorganic phosphorus diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0107] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0108] Stabilizer 500mmol / L

[0109] Reduced coenzyme 0.25mmol / L

[0110] Adenosine diphosphate 5mmol / L

[0111] Oxaloacetic acid 30mmol / L

[0112] Ammonia ion 30mmol / L

[0113] Pyruvate carboxylase 10000U / L

[0114] Alanine dehydrogenase 12000U / L

[0115] Glycine oxidase 12000U / L

[0116] Peroxidase 30000U / L

[0117] 4-amino antiarsenic 2mmol / L

[0118] Carbolic acid 10mmol / L

[0119] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0120] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the measured inorganic phosphorus sample and r...

Embodiment 2

[0123] The inorganic phosphorus diagnosis / measurement reagent of this embodiment is a double reagent, comprising:

[0124] Reagent 1

[0125] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0126] Stabilizer 50mmol / L

[0127] Reduced coenzyme 0.25mmol / L

[0128] Adenosine diphosphate 5mmol / L

[0129] Oxaloacetic acid 30mmol / L

[0130] Ammonia ion 30mmol / L

[0131] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0132] Reagent 2

[0133] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0134] Stabilizer 500mmol / L

[0135] Pyruvate carboxylase 10000U / L

[0136] Alanine dehydrogenase 12000U / L

[0137] Glycine oxidase 12000U / L

[0138] Peroxidase 30000U / L

[0139] 4-amino antiarsenic 0.6mmol / L

[0140] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0141] Set on the automatic biochemical analyzer: temperature 37°C, reactio...

Embodiment 3

[0144] The inorganic phosphorus diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0145] Reagent 1

[0146] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0147] Stabilizer 50mmol / L

[0148] Reduced coenzyme 0.25mmol / L

[0149] Adenosine diphosphate 5mmol / L

[0150] Oxaloacetic acid 30mmol / L

[0151] Ammonia ion 30mmol / L

[0152] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0153] Reagent 2

[0154] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0155] Stabilizer 500mmol / L

[0156] Alanine dehydrogenase 12000U / L

[0157] Glycine oxidase 12000U / L

[0158] Peroxidase 30000U / L

[0159] Dimethylaniline 2mmol / L

[0160] Reagent 3

[0161] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0162] Stabilizer 500mmol / L

[0163] Pyruvate carboxylase 10000U / L

[0164] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three r...

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Abstract

The invention relates to a kit for diagnosing / mensurating inorganic phosphorus by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the inorganic phosphorus, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamine, adenosine diphosphate, pyruvic acid, glutamine synthetase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the inorganic phosphorus directly.

Description

technical field [0001] The invention relates to an inorganic phosphorus diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of inorganic phosphorus, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] 87% of the phosphorus in the human body exists in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. Normal people’s blood calcium increases and blood phosphorus decreases, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma remains within a certain range; the product of calcium and phosphorus concentrations per 100 ml of plasma is about 35--40. When the product of the two is greater than 40, calcium and phosphorus are deposited in the bone tissue in th...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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