Method and reagent box for predicting dihydrochlorothiazide antihypertensive efficacy

A technology of hydrochlorothiazide and a kit is applied in the field of medical molecular biology, which can solve the problems of reduced diuresis and antihypertensive effect, and achieve the effects of high representativeness, low cost and significant prediction effect.

Inactive Publication Date: 2008-12-24
FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the function of OAT1 is reduced, it will reduce the secretion of hydrochlorothiazide from the blood into the renal tubules, resulting in a decrease in its diuretic and antihypertensive effects
[0005] There is currently no report on the polymorphism of SLC22A6 gene in the prediction of drug efficacy in hypertension

Method used

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  • Method and reagent box for predicting dihydrochlorothiazide antihypertensive efficacy
  • Method and reagent box for predicting dihydrochlorothiazide antihypertensive efficacy
  • Method and reagent box for predicting dihydrochlorothiazide antihypertensive efficacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Correlation study between SNP sites and hydrochlorothiazide antihypertensive efficacy

[0027] 1. Clinical trials:

[0028] Unrelated hypertensive patients aged 40-75 who had never received any hypertensive drug treatment or had not received any drug treatment in the last 8 weeks were enrolled from rural Xinyang, Henan. The diagnostic criteria for hypertension are systolic blood pressure ≥ 140mmHg and / or diastolic blood pressure ≥ 90mmHg at three different times within 2 months. The investigation process includes ①questionnaire basic information and medical history, traditional risk factors such as smoking and drinking, ②measurement of sitting blood pressure in both upper limbs, ③taking fasting venous blood samples, ④taking urine samples, ⑤measurement of height, weight, waist circumference, and hip circumference, ⑥Medical auscultation and physical examination, ⑦12-lead electrocardiogram, ⑧Echocardiography, ⑨Dispense medicine according to the random number, a...

Embodiment 2

[0093] Example 2: A kit for predicting the antihypertensive efficacy of hydrochlorothiazide

[0094]1. Composition: The composition and content are as follows (50 servings), stored at -20°C:

[0095] 50 μL 10×PCR buffer (TaKaRa),

[0096] 50μL 10mmol / L dNTP mixture (TaKaRa),

[0097] 12 μL (5U / uL) Taq DNA polymerase (TaKaRa),

[0098] Each 20μL (10umol / L) of SEQ ID NO.1 and SEQ ID NO.2 primers (Beijing Aoke Biological Company)

[0099] 5ml deionized double distilled water (ddH2O),

[0100] 100 μL 10×Sac I restriction endonuclease reaction buffer (New England Biolabs, USA)

[0101] 35 μL (10U / μL) restriction endonuclease Sac I (New England Biolabs, USA)

[0102] 2. How to use

[0103] 1. Extract the subject's genomic DNA by conventional methods;

[0104] 2. PCR reaction:

[0105] Table 4: PCR reaction system

[0106]

[0107] Reaction conditions:

[0108] step 1=94.0℃ for 5 minutes

[0109]

[0110] step 5 = 72.0°C for 10 minutes

[0111] step 6=4.0℃

[0112]...

Embodiment 3

[0121] Example 3: Study on the predictive effect of the kit on the antihypertensive efficacy of hydrochlorothiazide

[0122] 1. In order to further verify the predictive effect of the kit on the antihypertensive effect of hydrochlorothiazide, 245 patients were selected in Shandong Rizhao according to the same inspection and standards as in Example 1, and 12.5 mg of hydrochlorothiazide was administered each time. Once a day, if the blood pressure is not up to standard after 2 weeks, the dose will be doubled, and the treatment will last for 8 weeks.

[0123] 2. Genotyping:

[0124] 1. The reagents and equipment used are exactly the same as in Example 1.

[0125] 2. Experimental steps:

[0126] (1) Genomic DNA was extracted from 4ml of peripheral blood using the Blood Genomic DNA Purification Kit (DP318-02), and all steps were performed according to the instructions.

[0127] (2) Genotype rs10792367 using the kit for predicting the antihypertensive efficacy of hydrochlorothiaz...

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Abstract

The invention discloses a method for predicting the blood pressure reducing effect of hydrochlorothiazide and a kit, belonging to the medical molecular biology field. The method for predicting the blood pressure reducing effect of hydrochlorothiazide is realized by measuring and determining SLC22A6 gene polymorphism site. The method has the advantage of providing scientific basis for the individual therapy of the patient with high blood pressure disease in the real sense.

Description

technical field [0001] The present invention relates to a method and a kit for predicting the antihypertensive curative effect of hydrochlorothiazide, more specifically to the use of SLC22A6 gene polymorphism site in predicting the antihypertensive curative effect of hydrochlorothiazide in hypertension, belonging to field of medical molecular biology. Background technique [0002] Hypertension is the largest independent risk factor for many cardiovascular diseases such as coronary heart disease and cerebral apoplexy. It is possible to reach 1.56 billion annually. In China, the prevalence of hypertension is 18.8%, and there are about 160 million hypertensive patients, and the rate of new cases is increasing at a rate of 3.5 million per year. Actively intervening in blood pressure and controlling high blood pressure through treatment can effectively reduce the incidence and death of stroke and heart disease. This is a very certain and widely accepted fact. Studies have show...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 惠汝太韩运峰王晓建樊晓寒孙凯陈敬洲
Owner FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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