Preparation method of skin spot pricking method diagnosis lung acariasis antigen liquid

Abstract

The invention discloses a preparation method for antigen liquid for diagnosing pulmonary acariasis by adopting the skin pricking method. The preparation method comprises the following steps: firstly, collecting flour contaminated with dust from a flour mill; secondly, inspecting and separating mites through a microscope and feeding the mites; thirdly, when the density of the mites reaches 2000 per gram of feed base, positioning the mites into a sieve to remove the impurities; fourthly, positioning the powder sieved in the third step into a flat bottom enamel disk and separating the powder mites; fifthly, positioning live powder mites into a nylon bag, and immersing the mites into acetone for degreasing; sixthly, after washing, positioning the powder mites on the surface of a dish uniformly, then positioning the mites into a warm box to obtain dried powder mites; seventhly, immersing the dried powder mites into 9 percent of sodium chloride, and positioning the mites into the environment at 4 DEG C; eighthly, taking out the sodium chloride liquid in the seventh step, mixing the obtained suspension liquid, and taking supernatant through centrifugation; and ninthly, filtering the supernatant for removing bacteria, and obtaining the antigen liquid of the powder mites. The antigen liquid greatly prolongs the storage time, causes no pain to the patient and small skin irritability when being used for diagnosing pulmonary acariasis, is widely applicable in various medical institutions, and provides good basis for the diagnosis of the pulmonary acariasis.

Description

technical field [0001] The invention relates to an antigen solution of dust mite farinae, which is a preparation method specially used for the diagnosis of lung acariasis by skin pricking method. Background technique [0002] Pulmonary acariasis is a parasitic disease caused by mites inhaled into the human body through the respiratory tract and parasitic in the lungs. This parasitic disease occurs all over the world. Its clinical symptoms are: cough, expectoration, chest tightness, shortness of breath, asthma, fatigue, irritability, low-grade fever, and general malaise. Because pulmonary acariasis is a parasitic disease, some diagnostic methods are not yet accurate, and some diagnostic methods are more complicated and expensive. Therefore, it has not been widely used in various medical institutions, and its symptoms are somewhat similar to those of lung diseases, so it is often misdiagnosed, which leads to delays in the best treatment period and brings greater suffering to...

AI Technical Summary

Problems solved by technology

Because lung acariasis is a parasitic disease, some diagnostic methods are not yet accurate, and some diagnostic methods are more complicated and expensive

Therefore, it has not been widely used in var...