Artificial recombined hexon protein A, constructing method thereof and use

A technology of recombinant protein and hexaplex, which is applied in the field of genetic engineering, can solve problems such as inability to see experimental data, specific sequence differences, and inability to meet the application requirements of antibody separation and purification.
CN101337986AInactive Publication Date: 2009-01-07GUANGZHOU KONCEN BIOSCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU KONCEN BIOSCI
Publication Date
2009-01-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of genetic engineering, and relates to recombinant hexamer protein A as well as the constitution method and the application thereof. The recombinant hexamer protein A has an amino acid sequence represented by SEQ ID NO.1 in a sequence list. The recombinant hexamer protein A has higher activity than those of the reported recombinant protein A and the natural protein A, and has high yield and relatively simple purification process, thereby preventing the risk of pathogenic bacteria of naturally extracted protein. Additionally, the recombinant hexamer protein A suits large-scale production.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and relates to an artificially recombined hexaplex protein A as well as its construction method and application. Background technique

[0002] The discovery of protein A was associated with IgG (immunoglobulin G) from the beginning. As early as 1940, Vevwey found that in some Staphylococcus aureus, it contained a protein that could form a precipitate with normal human serum in a two-way diffusion test. substance. Jensen (1959) also found a similar phenomenon and named it A antigen. It was not until 1963 that Lofkvist et al. isolated the antigen and proved that it is a protein. To distinguish it from sugar, Grov (1960) named it staphylococcal protein A, referred to as SPA or protein A. In 1978, some researchers used heat-inactivated and formalin-fixed Staphylococcus aureus for the treatment of a type of cancer, which showed certain curative effect. This was the first time in the wor...

Claims

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