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Rapid checking method for bladder cancer by quantum dot mark immunity-chromatography test paper

An immunochromatographic test strip and quantum dot technology, applied in the field of medical testing, can solve the problems of delayed treatment, high examination fees, and patient pain, and achieve the effects of low detection limit, low cost, and wide application

Inactive Publication Date: 2009-01-07
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, it is very painful for patients to be tested by cystoscopy, and many patients are unwilling to do the test, which delays their condition; while ultrasound and CT tests are not only expensive, but also cumbersome.

Method used

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  • Rapid checking method for bladder cancer by quantum dot mark immunity-chromatography test paper

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention will be further described below by embodiment.

[0020] 1. Sample preparation and test strip production:

[0021] 1. Quantum dot-labeled antibody

[0022] Take 150uL of phosphate solution containing bovine serum albumin and sucrose at pH 7.4 and 10uL of quantum dots with carboxyl groups on the surface, use 10mg N-hydroxysulfosuccinic acid imide and 10mg 1-(3-dimethylaminopropyl )-3-ethylcarbodiamine hydrochloride as a coupling reagent, add monoclonal anti-human fibronectin 150uL and react on a shaker for 2 hours, and the reaction product is filtered through a sephadex-G-100 (1×35cm) chromatographic column , remove unreacted antibodies, centrifuge and purify, block with 4% bovine serum albumin, and store at 4°C for later use.

[0023] 2. Glass fiber membrane and nitrocellulose membrane treatment

[0024] Quantum dot-labeled monoclonal anti-human fibronectin was evenly sprayed on the glass fiber membrane, dried at room temperature, sealed, and sto...

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Abstract

The invention discloses a method for rapidly detecting the bladder cancer with quantum dot labeling immune chromatographic test paper. The existing method for bladder cancer detection comprises the cystoscope, ultrasonic waves, CT, and the like, but the methods are trivial in detection procedures and patients are subjected to pains. By making use of the quantum dot labeling immune chromatographic test paper, the method rapidly detects the amount of fibronectin protein contained in urine of bladder cancer patients and diagnoses and judges the bladder cancer. The detection method which is low in cost and simple in method is suitable for samples of blood, urine, saliva, and the like, can be applied to the detection of hormones, proteins, viruses, drugs, bacteria, environmental pollution, and the like, and can be used in places such as hospitals, customs, , airports and families.

Description

technical field [0001] The invention belongs to medical testing technology, in particular to a method for rapidly detecting bladder cancer with quantum dot-labeled immunochromatographic test paper. Background technique [0002] Bladder cancer is a malignant tumor disease with a high mortality rate. There are hundreds of thousands of new bladder cancer cases worldwide every year, and the prevalence rate is getting higher and higher. It has become one of the important causes of death. Early detection and timely diagnosis are effective means to save bladder cancer patients. The most commonly used methods for bladder cancer detection in modern medicine are cystoscopy, ultrasound, and CT. However, it is very painful for patients to be tested by cystoscopy, and many patients are unwilling to perform the test, which delays their condition. In addition to the high cost of examination, the procedures of ultrasound and CT detection are also cumbersome. Therefore, inventing a simple ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/574G01N33/533
Inventor 沈鹤柏方菲
Owner SHANGHAI NORMAL UNIVERSITY
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