Cell separation microchip based on silicon structure on insulators

Abstract

The invention provides a cell separation microchip based on a silicon structure on an insulator, which consists of two mutually combined layers, wherein, the first layer adopts a path layer, and the second layer adopts a cover plate layer. A micro-structure is formed on the path layer through engraving grooves on an SOI silicon chip, the path layer is provided with a micro channel, a liquid storage tank, an optical fiber groove and a microelectrode couple structure, and the liquid storage tank on the path layer is used for sieving the former and the later sample liquids and storing sheath flow liquid; the micro channel is unimpeded and used for sample (cell suspension or solution) flow; optical fibers provide an optical detecting circuit; the microelectrode couple is used for realizing cell sieving. A sample inlet and a sample outlet on the cover plate layer are connected with an external pipe. The information of the cell volume can be obtained by the optical fibers for distinguishingwhether the cell is a fused cell or not, alternating current photo-electric signals are applied on the micro electrode couple correspondingly to deflect the cells, so the cell separation is realized. The chip utilizes an electric field to implement the non-contact sieving and separating operations to the biological live cells, so the survival rate of live cells is enhanced, the structure is simple, the process is convenient, and the cost is low.

Description

technical field [0001] The invention relates to a microchip for cell screening and separation, in particular to a cell separation microchip based on a silicon-on-insulator (SOI) structure. Background technique [0002] Biological cells can form new cells through fusion, which is of great significance in the basic field of modern biomedical engineering. Screening and separation of fused cells is a prerequisite for further culturing of fused cells, and effective screening and separation of fused samples is of great significance for cell fusion. At present, there are many methods that can realize cell screening and separation, such as fluorescence separation, DC electric field deflection, optical clamp, micromanipulation and so on. These methods usually require complex structures or delicate operations, are quite difficult to implement, cause great damage to cells, have poor experimental results, and are difficult to promote. [0003] When two homologous cells fuse, the volum...

AI Technical Summary

Problems solved by technology

[0006] At present, there is little research and development of cell separation chips in China. On the one hand, it is difficult for the existing cell separation chips to detect without adding any dyes or fluorescent materials (these will damage the cells and affect the biological activity of the cells); On the other hand, the electric field strength and electric field gradient produced by the microelectrode are relatively weak, which is relatively weak in terms of precise control of cells, and the selection of processing materials is also weak in corrosion resistance and oxidation resistance. The present invention can better solve the above problems

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