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Separation and extraction method for camptothecine

An extraction method and technology for camptothecin, which are applied in the field of extracting natural active ingredients, can solve the problems of large investment in production equipment, complex production process, low product yield and the like, and achieve easy processing capacity, high separation efficiency, and small equipment volume. Effect

Inactive Publication Date: 2009-01-21
桂林普兰德生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The applicant has found through research that the product yields of process 1 and process 4 are all low, about 2 / 10000; Critical carbon dioxide extraction, the production process is relatively complicated, the investment in production equipment is large, and the cost is high; although the yield of the product in process 5 can reach 4 / 10000, the prerequisite is that the content of camptothecin in the selected raw materials must reach 8 / 10000~10 / 10000 Campotaphyllum fruit, and because only Campotaphyta fruit is used as raw material, it is also subject to seasonal restrictions

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  • Separation and extraction method for camptothecine

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Effect test

Embodiment 1

[0026]Get 10 kilograms of camptothecin content and be 7 / 10000~8 / 10000 camptothecin dry product, pulverize to 20 order; First use 150 liters of mass concentration to be the sodium hydroxide solution of 0.5% leaching at normal temperature for 2 hours, separate Go out camphor tree fruit; Add 200 liters of mass concentration again and be that the sodium hydroxide solution of 0.5% leach at normal temperature for 2 hours, isolate the camphor tree fruit; Then add 150 liters of mass concentration and be 0.5% sodium hydroxide solution at normal temperature Under leaching for 2 hours, isolate the philodendron citrus fruit, and combine the extracts from the three extractions; adjust the pH value to neutral with hydrochloric acid, and then carry out coarse filtration through a 120-mesh filter cloth; Use a 30nm ceramic membrane for microfiltration; perform ultrafiltration with a molecular weight cut-off of 10,000 on the leach solution after microfiltration; conduct nanofiltration concentrat...

Embodiment 2

[0028] Get 100 kilograms of camptothecin content and be 3 / 10000~4 / 10000 camptothecin dry product, be pulverized to 10~20 order; First use 2000 liters of mass concentrations to be 1.0% sodium hydroxide solution leaching at normal temperature for 3 hours, Isolate the leaves of Campylodia radiata; add 2000 liters of mass concentration of 1.0% sodium hydroxide solution to extract for 2 hours at normal temperature, isolate the leaves of Campylodia radiata; add 2000 liters of sodium hydroxide solution of 1.0% in mass concentration at normal temperature After leaching for 2 hours, separate the leaves of philodendron chinensis, and combine the extracts from three extractions; adjust the pH value to neutral with sulfuric acid, and then carry out coarse filtration through a 300-mesh filter cloth; use 30nm The metal membrane is used for microfiltration; the leach solution after microfiltration is subjected to ultrafiltration with a molecular weight cut-off of 10,000; the leach solution af...

Embodiment 3

[0030] Get 500 kilograms of camptothecin content and be the camptothecin leaf dry product of 6 / 10000, first use 8000 liters of mass concentration after the coarse powder as 1.0% sodium hydroxide solution to extract 3 hours at normal temperature, separate camptothecin leaf; Add 10000 1 liter of mass concentration is that the sodium hydroxide solution of 1.5% extracts 2 hours at normal temperature, and isolates the leaves of philodendron; adding 7500 liters of mass concentration is 0.5% sodium hydroxide solution and extracts for 3 hours at normal temperature, and isolates the leaves of eucalyptus Leaves, combined the extracts of three extractions; adjusted its pH value to neutral with acetic acid, and then carried out coarse filtration through a 200-mesh filter cloth; microfiltered the extracts after the coarse filtration with a 30nm ceramic membrane; The extract after microfiltration is subjected to ultrafiltration with a molecular weight cut-off of 10,000; the extract after ult...

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Abstract

The invention relates to the natural active ingredient extraction field from plants, in particular to a method for separating and extracting camptothecin. The technological line of the invention is as follows: common camptotheca fruits and common camptotheca leaves are taken as materials, and the technological steps are as follows: crushing, leaching of dilute sodium hydroxide solution, acid precipitation and impurity removal of the leaching solution, micro-filtration, ultrafiltration, nanofiltration, column chromatography by macropore adsorbent resins, desorption by ethanol and condensation of a stripping liquid to an extractive, extraction by ethyl acetate and crystallization of a extraction liquid to obtain a coarse product of camptothecin,recrystallization of a mixing liquid of the ethyl acetate and the acetone to obtain refined products of camptothecin. The method for separating and extracting camptothecin of the invention can fully leach out active ingredients of materials by multiple leaching, and after the processes of micro-filtration, ultrafiltration, nanofiltration concentration, column chromatography by macropore adsorbent resins and multiple crystallization, the extraction rate of the invention is 70 to 80 percent against the content of active ingredient camptothecin of the materials, and the purity of products extracted is more than 98 percent.

Description

(1) Technical field: [0001] The invention relates to the field of extracting natural active ingredients from plants, in particular to a separation and extraction method for preparing camptothecin from camptothecin fruits and camptothecin leaves as raw materials. (two) background technology: [0002] Camptothecin (CPT), is light yellow flaky crystal, molecular formula is C 20 h 16 N 2 o 4 , the molecular weight is 348.3, and the molecular structure formula is: [0003] [0004] Camptotheca acuminata Demce (Camptotheca acuminata Demce) is a tall deciduous tree endemic to my country, belonging to the family Cucumaceae. It is widely distributed in the Yangtze River Basin and southern provinces, and Guangxi is an important main producing area. In 1966, Monroe E.Wall in the United States isolated camptothecin for the first time from camptotheca japonica extract. Tumor tests proved that this tryptophan-terpene alkaloid has anticancer activity, which has attracted widespread ...

Claims

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Application Information

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IPC IPC(8): C07D491/22A61P35/00
Inventor 吕光政陈元胜韦彩珠庞显庭陈莲庞玉萍
Owner 桂林普兰德生物科技有限公司
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