Micro-capsule embedded with immobilized dominant bacteria, preparation and use thereof

A technology of dominant bacteria and microcapsules, applied in biochemical equipment and methods, chemical instruments and methods, methods based on microorganisms, etc., can solve complex and intense chemical reaction processes, weak water impact resistance, low mechanical strength of gels, etc. problems, to achieve the effects of low cost, improved mass transfer performance and mechanical strength performance, and convenient operation

Inactive Publication Date: 2009-01-21
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption method refers to the method in which microorganisms are adsorbed on the surface of the carrier and fixed. The biofilm method is a representative example. The adsorption method is limited due to its weak water shock resistance; the covalent bonding method and cross-linking method are complicated and intense due to the chemical reaction process. , so these two methods are basically not used in sewage treatment; the existing embedding technology affects its application due to its poor mass transfer performance and low mechanical strength of the gel

Method used

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  • Micro-capsule embedded with immobilized dominant bacteria, preparation and use thereof
  • Micro-capsule embedded with immobilized dominant bacteria, preparation and use thereof

Examples

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Embodiment 1

[0014] A microcapsule embedded with immobilized dominant bacteria, the microcapsule is composed of an inner core and an outer membrane, the inner core of which is calcium alginate gel beads, and the calcium alginate gel beads contain nano-dioxide Silicon material (specific surface area is 1000m 2 / g) fixed dominant bacteria, the inner core is covered with a chitosan film, the chitosan film is covered with a sodium alginate film, and the dominant bacteria contained in the inner core material is Pseudomonas alcaligenes.

[0015] The microcapsules of the present embodiment are prepared by the following methods and steps:

[0016] (1) Screening of dominant bacteria: collect activated sludge under domestic sewage, and domesticate the activated sludge by synchronous culture method; separate and concentrate the domesticated and mature activated sludge to make bacterial suspensions of different concentrations. After streaking and separating the bacterial suspension on the flat plate,...

Embodiment 2

[0022] A microcapsule embedded with immobilized dominant bacteria, the microcapsule is composed of an inner core and an outer membrane, the inner core of which is calcium alginate gel beads, and the calcium alginate gel beads contain nano-dioxide Silicon material (specific surface area is 1200m 2 / g) Fixed dominant bacteria, the inner core is covered with a chitosan film, the chitosan film is covered with a sodium alginate film, and the dominant bacteria contained in the inner core material is Gordonia amicalis.

[0023] (1) Screening of dominant bacteria: collect activated sludge under domestic sewage, and domesticate the activated sludge by synchronous culture method; separate and concentrate the domesticated and mature activated sludge to make bacterial suspensions of different concentrations, After streaking and separating the bacterial suspension on the flat plate, a dominant bacterium and Gordonia goodensis that efficiently degrade papermaking wastewater were screened ou...

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Abstract

The invention discloses a microcapsule embedded with fixed dominant bacteria. The microcapsule consists of an inner core and a membrane which is coated on the outside, wherein the inner core is a calcium alginate gel bead which comprises dominant bacteria with immobilized nanophase materials; the chitosan membrane is coated on the outside of the inner core; and a sodium alginate membrane is coated on the outside of the chitosan membrane. The microcapsule is prepared through steps of screening of the dominant bacteria, immobilization of the dominant bacteria and so on, and can be effectively applied to treatment of wood pulp paper-making waste water. The method for preparing the microcapsule is simple and has low cost; and the microcapsule prepared has greatly improved mass transfer performance and mechanical strength and has good effect on processing the paper-making waste water.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of sewage, and in particular relates to a microcapsule embedding immobilized bacteria and its preparation and application in sewage treatment. Background technique [0002] The sewage from high-yield wood pulp and paper mills can be treated in the same way as other papermaking sewage, and the treatment technologies are diversified, but biological treatment is an indispensable and important part. Biological treatment is an economical, reasonable and effective means of water pollution control. At present, the commonly used methods include activated sludge method and biofilm method, and enhanced biotechnology has gradually become a research hotspot, such as the immobilization of microorganisms. At present, the immobilization of microorganisms mainly includes four categories: adsorption method, covalent bonding method, cross-linking method, and embedding method. The adsorption method refe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C02F3/34C12R1/38C12R1/01C02F103/28
Inventor 陈介南熊晓妹何钢林佳施英乔
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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