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Extraction method of hericium erinaceus polysaccharide

A technique for extracting polysaccharides from Hericium erinaceus, applied in the field of extracting polysaccharides from Hericium erinaceus, can solve problems such as difficult removal and easy clogging of ultrafiltration membrane pores, achieve fast ultrafiltration speed, facilitate industrial production, and have high biological activity Effect

Active Publication Date: 2011-04-27
ZHEJIANG ESSENCE FUNGI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liu Zhenjun's invention patent "Fungal Polysaccharide Refining and Purification Process" pointed out the method of purifying fungal polysaccharides (200610012682.3), but for Hericium erinaceus polysaccharides, there are darker phenolic compounds connected to sugar chains, which are difficult to remove. Moreover, there are also problems such as the interference of biological macromolecules such as cellulose and pectin, and the pores of the ultrafiltration membrane are easily blocked.
Therefore, the Hericium erinaceus polysaccharide obtained by this method also has the defects that product color, efficiency, purity, etc. need to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. To pretreat the fruiting bodies of Hericium erinaceus, take fresh Hericium erinaceus, air-dry it naturally, fully pulverize it, and pass through a 60-mesh sieve.

[0023] b. Add 10 times the quality of distilled water to the sieved raw material, stir evenly and gradually heat up, use 10KHz ultrasonic wave to extract it for 1.5h in the range of 60°C, filter, repeat the above steps for the filter residue, and extract once more, Combine the filtrates.

[0024] c. Adjust the pH of the filtrate to 4.0, first add 0.8 times the mass of cellulase (enzyme activity 15000U / g, Wuxi Xuemei Enzyme Technology Co., Ltd., the same below), control the temperature at 40°C, hydrolyze for 1.5h, freeze and centrifuge , discard the residue; adjust the pH of the filtrate to 4.0, continue to add 1.0 times the mass of pectinase (enzyme activity 20000U / g, Tianjin Lihua Enzyme Preparation Factory, the same below) to the filtrate, control the temperature at 45 ° C, hydrolyze 1.0h, freeze and ce...

Embodiment 2

[0034] a. Pretreatment of the fruiting body of Hericium erinaceus, take fresh Hericium erinaceus, air-dry it naturally, fully pulverize it, and pass through a 100-mesh sieve.

[0035] b. Add 20 times the mass of distilled water to the sieved raw material, stir evenly and gradually heat up, use 15KHz ultrasonic wave to extract it for 0.8h in the range of 50°C, filter, repeat the above steps for the filter residue, and extract once more, Combine the filtrates.

[0036] c. Adjust the pH of the filtrate to 4.0, first add 0.6 times the mass of cellulase, control the temperature at 45 ° C, hydrolyze for 1.0 h, refrigerate and centrifuge, discard the residue; then adjust the pH of the filtrate to 4.5, and continue to add 1.0 times the mass to the filtrate pectinase, control the temperature at 50°C, hydrolyze for 1.0h, refrigerate and centrifuge, discard the residue; finally adjust the pH of the filtrate to 4.5, add 1.2 times the mass of papain, control the temperature at 45°C, hydrol...

Embodiment 3

[0046] a. Pretreatment of Hericium erinaceus fruiting body, take fresh Hericium erinaceus, air-dry it naturally, fully pulverize it, and pass through an 80-mesh sieve.

[0047] b. Add 25 times the quality of distilled water to the sieved raw material, stir evenly and gradually heat up, use 15KHz ultrasonic wave to extract it for 0.8h in the range of 60°C, filter, repeat the above steps for the filter residue, and extract once more, Combine the filtrates.

[0048] c. Adjust the pH of the filtrate to 3.5, first add 0.9 times the mass of cellulase, control the temperature at 45°C, hydrolyze for 2.0 hours, refrigerate and centrifuge, and discard the residue; then adjust the pH of the filtrate to 4.5, and continue to add 1.2 times the mass to the filtrate Pectinase, control the temperature at 45°C, hydrolyze for 1.0h, refrigerate and centrifuge, discard the residue; finally adjust the filtrate to pH 4.0, add 1.0 times the mass of papain, control the temperature at 45°C, hydrolyze f...

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PUM

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Abstract

The invention provides a hericium erinaceus polysaccharide extraction method with high product yield and good purity. The method comprises that: hericium erinaceus dried powder is added with water, is extracted by 8 to 20 KHz ultrasonic under the temperature of 50 to 80 DEG C for 0.5 to 2.0 hours, and is filtered; the filtrate is hydrated orderly by sufficient cellulose, pectinase and papain, finally is added with hydrogen peroxide to be stirred in heat preservation under the temperature of 30 to 50 DEG C for 0.5 to 1 hour, and is hyperfiltrated by a hyperltration membrane; the components with the molecular weight of more than 10000 dalton are collected, frozen and dried to obtain the hericium erinaceus polysaccharide. The hericium erinaceus polysaccharide extraction method has the advantages that 1. the prepared product has high purity; wherein, the content of Beta-dextran is as high as more than 40 percent and the biological activity is high, and the hericium erinaceus polysaccharide yield can reach 12.2 percent; 2. the hydrogen peroxide is adopted to decolorize, the conditions are mild, the polysaccharide structure is difficult to damage and the by-product is water, the method is green and environment-friendly, and coincides with the production requirements of the health care food.

Description

(1) Technical field [0001] The invention relates to a method for extracting Hericium erinaceus polysaccharide. (2) Background technology [0002] Hericium erinaceus (Hericium erinaceus), also known as Hericium erinaceus, Hericium erinaceus, Hericium erinaceus, etc., is a large fungus, belonging to Basidiomycetes, Polyporaceae, Odontomycetes, and Hericium genus. The entity is named after its shape resembling a monkey's head. The polysaccharide extracted from the fruiting body of Hericium erinaceus is a glucan mixture whose main chain is connected by β-1,3 glycosidic bonds and branched chains are connected by β-1,6 glycosidic bonds. The molecular weight is between 8000 and 40000. A large number of medical and pharmacological studies have shown that the polysaccharide of Hericium erinaceus has physiological functions such as improving immunity, anti-tumor, anti-aging, and lowering blood lipids, and has curative effects on canceration of the digestive system, ulcers, gastric si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/04
Inventor 贺亮吴学谦程俊文吴庆其付立忠李海波魏海龙
Owner ZHEJIANG ESSENCE FUNGI DEV
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