Monoclonal antibody IE5 for detecting new castle disease virus variation strain

Abstract

The invention provides a monoclonal antibody for detecting new-castle disease virus variant. The preparation method comprises the following steps: immunizing a BALB / C mouse of eight ages using purified new-castle disease virus NDV LaSota strain as immunogen, boostering immnunization, standing for three days, and taking mouse splenic cells to fuse with myeloma cells SP2 / 0 in a logarithmic growth phase. The purified NDV LaSota strain is antigen coated enzyme marking board for screening positive Hybridoma cell, and the monoclone 1E5 is positive via ELISA detection, and is subcloned, and then injected into an abdominal cavity of a sensitized BALB / C mouse of 8 to 10 ages for preparing ascites. The mab bioactivity identification result indicates that mab 1E5 ascites has ELISA titer of 100 multiply 2<10>, immunoglobulin subclass of IgG2a, HI titer of 14log2, and cell neutralization titer for NDV standard virulent strain F48E8 of 320. The monoclonal antibody can be used for antigenic variation research of the new-castle disease virus to monitor and forecast epidemic situation of new castle disease, and provide scientific theoretical basis for the effective prevention and control of the new castle disease.

Description

technical field [0001] The patent of the present invention relates to a diagnostic reagent in the field of veterinary biotechnology, specifically a monoclonal antibody 1E5 for detecting variant strains of Newcastle disease virus. Background technique [0002] Newcastle disease (ND) is a highly contagious and highly contagious disease of poultry, which has caused serious economic losses to the poultry industry in many countries. The causative agent of Newcastle disease is Newcastle disease virus (NDV), a member of the Paramyxoviridae family Mumpsvirus, and a single-stranded non-segmented RNA virus with an envelope. The hemagglutinin-neuraminidase (HN) and fusion protein (F) on the surface of NDV are the main immunoprotective antigens and are closely related to the pathogenicity of the virus. HN protein mainly mediates virus recognition of cell surface receptors, F protein promotes the fusion of viral envelope and cell membrane, and HN protein also participates in the process...

AI Technical Summary

Problems solved by technology

In recent years, domestic NDV molecular epidemiological research results have shown that the popular NDV in my country has both old genotypes (such as genotype II) and new genotypes (such as genotype VII). However, the new genotype NDV Has the antigenicity of the virus changed? Due to different experimental conditions and experimental strains in different laboratories, the research results are quite different

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