Active regeneration artificial cornea graft and preparation method thereof

A technology of artificial corneas and grafts, applied in eye implants, medical science, prostheses, etc., can solve problems such as hard and opaque corneas, failed corneal transplants, and intraocular inflammation

Inactive Publication Date: 2009-03-11
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main problem of this method is that after a series of biochemical treatments, the animal cornea is hard and opaque, and the implantation produces immune rejection, which causes angiogenesis in corneal tissue and intraocular inflammation, resulting in failure of corneal transplantation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Active regenerative artificial corneal grafts include the following components by weight or mass concentration

[0045] Chitosan: 10 parts; Collagen: 10 parts;

[0046] Chondroitin sulfate: 0.3 parts; Triple distilled water: 67 parts;

[0047] Polyethylene glycol (molecular weight 1000): 5 parts;

[0048] Polyvinyl alcohol (molecular weight 10000): 10 parts;

[0049] Keratocyte growth factor: 10ng / ml;

[0050] Fibronectin: 60ng / ml;

[0051] Laminin: 25ng / ml;.

[0052] The composition of keratocyte growth factor is fibroblast growth factor (bFGF) 10ng / ml.

[0053] The above-mentioned active regenerative artificial corneal graft is prepared by the following steps: (1) weigh chitosan, collagen and chondroitin sulfate according to the above ratio, dissolve chitosan and collagen in hydrochloric acid solution or acetic acid solution with pH<3 respectively, sulfuric acid Dissolve chondroitin in triple distilled water; (2) Mix the above components and stir at room tempera...

Embodiment 2

[0056] By weight or mass concentration, the active regenerative artificial keratoplasty comprises the following components:

[0057] Chitosan: 20 parts; Collagen: 25 parts;

[0058] Chondroitin sulfate: 0.2 parts; Triple distilled water: 45 parts;

[0059] Polyethylene glycol (molecular weight 4000): 5 parts;

[0060] Polyvinyl alcohol (molecular weight 40000): 5 parts;

[0061] Keratocyte growth factor: 65ng / ml;

[0062] Fibronectin: 70ng / ml;

[0063] Laminin: 25ng / ml;

[0064] Among them, the composition of keratocyte growth factor is fibroblast growth factor (bFGF) 20ng / ml, epidermal growth factor (EGF) 30ng / ml, transforming growth factor (TGF-β 1 ) 15ng / ml.

[0065] The above-mentioned active regenerative artificial corneal graft is prepared by the following steps: (1) weigh chitosan, collagen and chondroitin sulfate according to the above ratio, dissolve chitosan and collagen in hydrochloric acid solution or acetic acid solution with pH<3 respectively, sulfuric acid...

Embodiment 3

[0068] By weight or mass concentration, the active regenerative artificial keratoplasty comprises the following components:

[0069] Chitosan: 30 parts; Collagen: 15 parts;

[0070] Chondroitin sulfate: 0.4 parts; Triple distilled water: 37 parts;

[0071] Polyethylene glycol (molecular weight 50000): 5 parts;

[0072] Polyvinyl alcohol (molecular weight 100000): 5 parts;

[0073] Keratocyte growth factor: 75ng / ml;

[0074] Fibronectin: 40ng / ml;

[0075] Laminin: 55ng / ml;

[0076] Among them, the composition of keratocyte growth factor is fibroblast growth factor (bFGF) 30ng / ml, epidermal growth factor (EGF) 20ng / ml, transforming growth factor (TGF-β 1 ) 25ng / ml.

[0077] The above-mentioned active regenerative artificial corneal graft is prepared by the following steps: (1) Weigh chitosan, collagen and chondroitin sulfate according to the above ratio, dissolve chitosan in hydrochloric acid solution with pH<3, dissolve collagen in pH<3 Acetic acid solution, chondroitin ...

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Abstract

The invention belongs to the technical field of artificial cornea production and more particularly provides an active regenerating artificial cornea implant and a preparation method thereof. The artificial cornea implant comprises 5-30 shares of chitosan, 5-30 shares of collagen, 0.1-0.5 share of chondroitin-sulfuric acid, 5-10 shares of polyethylene glycol, 5-25 shares of polyvinyl alcohol and 15-80 shares of triple-distilled water; preferably the artificial cornea implant also comprises appropriate amount of cornea cell growing factor, fibronectin and laminin. The preparation method of the active regenerating artificial cornea implant is as below: dissolving the chitosan, the collagen and the chondroitin-sulfuric acid in hydrochloric acid, acetic acid and water respectively, and then infusing the mixture of the components which are agitated uniformly at the room temperature into a mould for drying, and then obtaining the product after water immersion, cleaning and drying. The artificial cornea implant has good biocompatibility, high substance rigidity and good transparency; the preparation method is easy to be operated, the raw materials are easy to be obtained and are cheap, and large-scale industrialized production can be performed.

Description

technical field [0001] The invention relates to the technical field of artificial cornea manufacturing, in particular to an active regenerative artificial corneal graft and a preparation method thereof. Background technique [0002] About 10 million corneal blind patients in the world need corneal transplantation, and there are about 3 million people in my country. However, only about 3,000 cases of allogeneic keratoplasty can be performed in my country every year. This is mainly because allogeneic corneas are more expensive for corneal transplantation and it is difficult to obtain donors. The shortage of donors makes it an urgent technical problem to develop artificial cornea as a substitute for allogeneic cornea. [0003] The current artificial corneas are mainly inactive corneas, which are generally made of synthetic materials such as methyl methacrylate (MMA). Although the implantation of such materials can restore people's vision, the field of vision is extremely smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/18A61L27/54A61F2/14
Inventor 李沁华陈建苏
Owner JINAN UNIVERSITY
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