Cherimoya inner ester extract as well as extraction method and use thereof in preparing anti-cancer medicine

A technology of anemone lactone and extract, which is applied in the extract and extraction of anemone lactone, the application of breast cancer and liver cancer drugs, and the preparation and treatment of lung cancer, which can solve the problems of unsatisfactory curative effect, low cancer curative effect, and large toxic and side effects and other issues, to achieve the effect of improving the clinical efficacy of anti-cancer and high anti-cancer activity

Inactive Publication Date: 2009-04-15
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapeutic drugs have a certain effect on cancer, but they have severe side effects

Method used

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  • Cherimoya inner ester extract as well as extraction method and use thereof in preparing anti-cancer medicine
  • Cherimoya inner ester extract as well as extraction method and use thereof in preparing anti-cancer medicine
  • Cherimoya inner ester extract as well as extraction method and use thereof in preparing anti-cancer medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the extraction method of anemone lactone extract

[0026] 20 kg of custard apple seeds were crushed into coarse powder, extracted three times with 95% ethanol under reflux, and the ethanol extract was concentrated under normal pressure to obtain 1.2 kg. Sequentially extract 3 times with 10 times the amount of petroleum ether (20-60° C.), water-saturated ethyl acetate, and chloroform. The combined extracts are concentrated and dried to obtain 610 g of anemone lactone extract.

[0027] After repeated silica gel column chromatography, the silica gel particle size is 100-300 mesh, and the mass ratio to the extract is 10:1-20:1; it is eluted with chloroform-methanol gradient with a volume ratio of 100:0-90:10, The collected annona lactone extract was concentrated and dried to obtain 230g. After several times of silica gel column chromatography, the silica gel particle size is 100-300 mesh, and the mass ratio to the annona lactone extract is 10:1-50:1; the volu...

Embodiment 2

[0028] Example 2: Inhibitory effect of annona lactone extract on transplanted lung cancer

[0029] 1. Experimental animals and tumor strains

[0030] Male healthy C57BL / 6 mice, weighing 18-22 g, aged 6-8 weeks, were purchased from the Animal Experiment Center of our school; mouse Lewis lung cancer tumor strains were purchased from the Animal Experiment Center of Jiangsu Cancer Institute.

[0031] 2. Modeling and experimental methods

[0032] There were 60 C57BL / 6 mice, 10 were randomly reserved in proportion as the negative control group, and the remaining 50 were used for modeling. Prepare Lewis lung cancer tumor-bearing mice, peel off the tumor tissue in the vigorous growth period, and select a well-growing tumor of 1.5mm 3 Left and right, homogenate according to the ratio of tumor (g): normal saline (ml) 1:3, adjust the number of cells to 2×10 6 / ml. Select healthy mice, and inoculate 0.2 ml subcutaneously in the right axilla of each mouse under aseptic conditions. The...

Embodiment 3

[0039] Example 3: Inhibitory effect of annona lactone extract on transplanted breast cancer

[0040] 2. Experimental animals and tumor strains

[0041] Female healthy TA2 mice, weighing 18-22 g, aged 6-8 weeks, were purchased from the Animal Experiment Center of our university; the mouse breast cancer cell line MA-891 was purchased from the Animal Experiment Center of Jiangsu Cancer Institute.

[0042] 2. Modeling and experimental methods

[0043] There were 60 TA2 mice, 10 were randomly reserved in proportion as the negative control group, and the remaining 50 were used for modeling. Take MA-891 mouse breast cancer cells in logarithmic growth phase cultured in vitro, and adjust the cell concentration to 1×10 7 / ml. Under sterile conditions, MA-891 mouse breast cancer cells were inoculated subcutaneously in the right axilla of TA2 mice, the inoculation volume was 0.2ml / mouse (the number of cells was 2×10 6 / Only). The entire inoculation process must be carried out in a st...

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Abstract

The invention relates to sweetsop lactone extract and an extraction method thereof. The sweetsop lactone extract is an anticancer active ingredient obtained after extraction and separation of sweetsop seeds. The extraction method comprises the following steps: firstly, organic solvents such as ethanol, petroleum ether, chloroform, and so on are used for extracting sweetsop lactone from coarse powder of the sweetsop seeds; and secondly, the sweetsop lactone is subjected to silica gel column chromatography, chloroform-methanol elutioin, petroleum ether-ethyl acetate elution and ethyl acetate-metanol elution to obtain the sweetsop lactone extract of which the content is between 50 and 90 percent of mass percent. As proved by in vivo anticancer pharmacological experiments of animals, the sweetsop lactone extract has obvious functions in inhibiting the growth of lung cancer cells, breast cancer cells and liver cancer cells, and can be used for preparing an anticancer medicine because the anticancer activity of the sweetsop lactone extract is 100 percent higher than that of a chemotherapeutic medicine - 5-fluorouracil.

Description

1. Technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to an annonaceae lactone extract extracted from cherimoya seeds as a raw material, an extraction method and an application of the extract in preparing medicines for treating lung cancer, breast cancer and liver cancer. 2. Background technology [0002] Cancer is a formidable enemy that endangers human health. There are about 1.6 million new cancer cases in my country every year, and about 1.3 million people die from cancer every year. The number of deaths due to cancer in the country is increasing year by year. my country is a high-incidence area for lung cancer, breast cancer, liver cancer and other cancers. Cancer mortality stay high. [0003] There are currently no effective drugs that cure most cancer patients. Although chemotherapeutic drugs have certain curative effects on cancer, they have severe side effects, and Chinese patent medicines generally have low curati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61K131/00
Inventor 章永红
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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