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Enzymic induction method for Agrocybe chaxingu extracellular lignocellulose and extracellular cellulase fermentation material

A technology of lignocellulase and cellulase, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of not well solving the problems of efficient production of cellulase

Inactive Publication Date: 2010-12-22
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, cellulase is widely used in various fields such as food industry, medical and health care, daily chemical industry, petroleum exploration and feed industry, so cellulase has a very broad application prospect. The key to the utilization of renewable resources, but so far, the problem of high-efficiency production of cellulase has not been well solved, and my country has a high market demand for cellulase, so screening bacteria with high enzyme activity is a top priority

Method used

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  • Enzymic induction method for Agrocybe chaxingu extracellular lignocellulose and extracellular cellulase fermentation material
  • Enzymic induction method for Agrocybe chaxingu extracellular lignocellulose and extracellular cellulase fermentation material
  • Enzymic induction method for Agrocybe chaxingu extracellular lignocellulose and extracellular cellulase fermentation material

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Experimental program
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Embodiment 1

[0020] A. Preparation of the first-class species of Agrocybe chaxingu Huang: Agrocybe chaxingu Huang No. 5 was provided by the Experimental Center for Culture Collection of Huazhong Agricultural University.

[0021] B, the preparation of the secondary species of Agrocybe chaxingu Huang:

[0022] Secondary seed medium: 1960 grams of wheat grains, 40 grams of calcium carbonate.

[0023] Mix the culture medium according to the formula and put it into 500 ml edible fungus polychloride plastic bags with suitable tightness, seal it with gauze twice and a layer of cotton, and sterilize at 121°C for 1.5 hours. Insert the first-level species, and cultivate it at a constant temperature of 25°C for 20 days to obtain the second-level species of Tea Salmonella.

[0024] C. Preparation of Agrocybe chaxingu Huang three-level basic compost: 38 grams of cottonseed hulls, 30 grams of sawdust, 18 grams of wheat bran, 8 grams of corn flour, 3 grams of bean cake, 0.5 grams of tea leaves, and 1.5 ...

Embodiment 2

[0036] Embodiment 2: For the experimental control, the difference from Example 1 is that no nutrient-rich components such as any composite carbon source and composite nitrogen source are added. The composition and content of the three-level kind of basic culture material are the same as in Example 1, and the tea salivary mushroom is determined Cellulase activity in tertiary seed basal compost.

Embodiment 3

[0037] Embodiment 3: The difference from Embodiment 1 is that the contents of complex carbon and nitrogen source nutrient-rich components are different in the three-level seed basal compost. Promptly add 46 milliliters of mannitol: CMC (carboxymethyl cellulose)=1:2 (mass ratio) that concentration is 5 grams / liter; Add concentration is 5 grams / liter yeast powder: urea=1:2 (mass ratio ) 11.5 ml.

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Abstract

The invention discloses an inducing method of an agrocybe chaxingu huang extracellular ligno-cellulose and an extracellular ligno-cellulose fermentation material. The inducing method includes the following steps of: preparing the first-grade seeds of the agrocybe chaxingu huang; preparing the second-grade seeds of the agrocybe chaxingu huang; preparing the basic culturing material of the third-grade seeds of the agrocybe chaxingu huang; preparing the compound concentrating culturing material of the third-grade seeds of the agrocybe chaxingu huang; and fermenting the compound concentrating culturing material of the third-grade seeds of the agrocybe chaxingu huang; in this way, the extracellular ligno-cellulose fermentation material is obtained. The invention starts from the point of view of enzymology and takes the new expansive edible agrocybe chaxingu huang as a production material for forming an integration technology which is mainly characterized by effectively producing the agrocybe chaxingu huang extracellular ligno-cellulose for achieving the largest conversion rate of biological energy sources so as to ease the phase of energy source shortage and to maintain the eubiosis.

Description

technical field [0001] The invention relates to a method for inducing extracellular lignocellulase of tea salamander. Background technique [0002] At present, cellulase is widely used in various fields such as food industry, medical and health care, daily chemical industry, petroleum exploration and feed industry, so cellulase has a very broad application prospect. The key to the utilization of renewable resources, but so far, the problem of efficient production of cellulase has not been well solved, and my country has a high market demand for cellulase, so screening high enzyme activity strains is a top priority. Contents of the invention [0003] The purpose of the present invention is to provide a method for inducing extracellular lignocellulosic enzymes by using tea salamander as raw material. [0004] Another object of the present invention is to provide a kind of extracellular cellulase fermented material obtained from the tea salami mushroom. [0005] The method f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/645C12N9/42
Inventor 路等学王龙王秉峰高静梅赵玉卉张铎
Owner GANSU ACAD OF SCI INST OF BIOLOGY