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Method for detecting chlorothalonil pesticide residue and detection kit therefor

A technology of chlorothalonil and complete antigen, which is applied in the field of chlorothalonil polyclonal antibody, detection of chlorothalonil pesticide residues in fruits and vegetables, and preparation of chlorothalonil artificial complete antigen, which can solve the problems that ELISA kits have not been reported, etc. Achieve the effect of low cost, high efficiency and low detection limit

Inactive Publication Date: 2009-04-22
TARIM UNIV
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Problems solved by technology

Kits for the detection of pesticide residues by ELISA emerge in endlessly, but ELISA kits for the detection of chlorothalonil have not been reported

Method used

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  • Method for detecting chlorothalonil pesticide residue and detection kit therefor
  • Method for detecting chlorothalonil pesticide residue and detection kit therefor
  • Method for detecting chlorothalonil pesticide residue and detection kit therefor

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Embodiment Construction

[0044] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0045]1. Molecular design and synthesis of hapten: For the synthesis route of chlorothalonil hapten and artificial complete antigen, see figure 1 . Accurately weigh 500 mg (1.9 mmol) of dried chlorothalonil and 273 mg (4.7 mmol) of potassium fluoride, dissolve in 20 mL of ethylene glycol solution, stir in an oil bath at 90 ° C, stop the reaction after 36 hours, and dissolve the reaction solution After cooling to room temperature, it was poured into 50 g of ice water. After the precipitation was complete, it was filtered with suction, washed with ice water, and dried in vacuo to obtain 262 mg of white powder. Elute with petroleum ether: acetone = 9: 1 (V / V) as the eluent, separate by column chromatography on a silica gel column (300-400 mesh), collect fractions in equal volume ...

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Abstract

The invention relates to a method for detecting ELISA remained in chlorothalonil pesticide and a reagent kit thereof. Glycol is utilized to substitute chloride atoms on 6 position carbon atoms of chlorothalonil molecules to synthesize a semiantigen; then an imidazolone method is adopted to link the semiantigen to a carrier, namely bovine serum albumin molecules to form an artificial complete antigen; an antigen-immunized rabbit is used to prepare a polyclonal antibody which is assembled with related reagents into an ELISA reagent kit; and an ELISA indirect method of antigen-primary antibody-ELIAS secondary antibody is used to detect residues of the chlorothalonil pesticide. The method has the advantages of low cost, high efficiency, and low detection limit; the detection of 96 samples at one time can be finished within four hours, and the lowest limit of the detection is 0.016mg.kg<-1>.

Description

technical field [0001] The invention relates to a method for detecting chlorothalonil pesticide residues in fruits and vegetables, and a chlorothalonil ELISA detection kit. At the same time, the invention also relates to a preparation method of chlorothalonil artificial complete antigen and a chlorothalonil polyclonal antibody, belonging to enzymes Linked immunoassay technology field. Background technique [0002] Chlorothalonil is a broad-spectrum fungicide, which has good adhesion on the surface of plants, is not easily washed by rain, and has stable efficacy. Its pure product is white crystal, odorless, insoluble in water, slightly soluble in acetone and other organic solvents, low toxicity to higher animals, but more toxic to fish and crustaceans, mainly used to prevent downy mildew of vegetables , Anthracnose, blight, etc. (Institute for the Control of Pesticides, Ministry of Agriculture. New Pesticide Handbook [M]. Beijing: Agricultural Press, 1989, 316-317.). [000...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K16/44G01N33/543G01N21/25
Inventor 张利莉白红进王彦芹万传星刘文杰陈伟赵小亮井波
Owner TARIM UNIV