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Method for preparing Rhodiola rosea solid-state transformation fermentate by using microbial mixing bacterial strain

A technology of mixed strains and rhodiola, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of reducing the consumption of raw medicinal materials

Inactive Publication Date: 2009-04-22
SICHUAN UNIV
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  • Abstract
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing Rhodiola solid-state conversion fermented product with microbial mixed strains, which can greatly improve The effective active ingredients in the original medicinal materials of Rhodiola can obtain more extraction products under the condition of using the same amount of original medicinal materials, so that the wild Tibetan medicinal materials can be fully utilized

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  • Method for preparing Rhodiola rosea solid-state transformation fermentate by using microbial mixing bacterial strain

Examples

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Effect test

Embodiment 1

[0033] This embodiment is the preparation of Aspergillus niger and Aspergillus oryzae mycelia.

[0034] 1) Mash the natural wild Rhodiola rosea medicinal material, take 1g, add 5ml of sterile water, shake well, and then dilute 10, 100, 1000 and 10000 times with sterile water respectively. Take 0.1ml of each of the above dilutions, spread them on the separation medium consisting of 10g of glucose, 5g of peptone, 5g of yeast extract, 1g of potassium dihydrogen phosphate, 0.5g of ammonium sulfate, 20g of agar and 1000ml of water. Cultivate for 3 days, pick a single colony, and select a bacterial strain whose content of salidroside is increased after being transformed and fermented in the Rhodiola rosea medium and detected by high performance liquid chromatography (HPLC).

[0035] 2) Mix Rhodiola rosea powder, bran and water with a particle size of 80 mesh at 0.8:0.2:1 by weight, and then steam sterilize to obtain the Rhodiola rosea bran seed culture medium, and then inoculate them ...

Embodiment 2

[0037] Crush 100g of Rhodiola rhizome raw materials to 80 mesh, then add 0.05g of yeast extract, 0.05g of potassium dihydrogen phosphate, 0.02g of magnesium sulfate and 100mL of water, stir and mix evenly to make it a solid culture material for Rhodiola rosea, and use steam Sterilize at 121 DEG C for 30 minutes; After cooling, inoculate the mixed mold mycelium of 5g (wet weight) prepared by the method of Example 1, wherein the weight ratio of Aspergillus niger (Aspergillus niger) and Aspergillus oryzae (Aspergillus oryzae) is 8:2, and then incubated and fermented at 28°C for 60 hours.

[0038] The obtained solid transformation fermented product is detected by high performance liquid chromatography (HPLC), and the content of salidroside is 1.1869%. Compared with the salidroside content of the original medicinal material detected under the same conditions as 0.7306%, the converted fermented product The content of salidroside can be increased by 62.46%.

Embodiment 3

[0040] Crush 100g of Rhodiola rhizome raw materials to 80 mesh, then add 0.1g of yeast extract, 0.1g of potassium dihydrogen phosphate, 0.03g of magnesium sulfate and 100mL of water, stir and mix evenly to make it a solid culture material for Rhodiola rosea, and use steam Sterilize at 121 DEG C for 30 minutes; After cooling, inoculate the mixed mold mycelium of 7.5g (wet weight) prepared by the method of Example 1, wherein the weight ratio of Aspergillus niger (Aspergillus niger) and Aspergillus oryzae (Aspergillus oryzae) 6:4, and then incubated and fermented at 30°C for 55 hours.

[0041] The obtained solid transformation fermented product is detected by high performance liquid chromatography (HPLC), and the salidroside content is 1.2469%. Compared with the salidroside content of the original medicinal material detected under the same conditions as 0.7306%, the converted fermented product The content of salidroside can be increased by 70.67%.

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Abstract

The invention provides a method for improving active ingredients in a rhodiola rosae raw medicinal material through the transformation fermentation by a mixed microorganism strain in a solid culture material of the rhodiola rosae raw medicinal material. The method comprises the following steps: the solid culture material of rhodiola rosae produced by rootstalk powder of the rhodiola rosae is sterilized and then is grafted with the mixed microorganism strain for solid fermentation culture. Through the transformation fermentation of the method, the active ingredients of the rhodiola rosae can be greatly improved than those of the raw medicinal material to further ensure that the content of the active ingredients is improved by more than 70 percent than that of the raw medicinal material at best, so the method has the advantages that the method can effectively utilize wild resources, can also reduce the discharge amount of pollutants such as medicine residues and so on at the same time, realize the protective development of traditional Tibetan medicines, and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of extracting effective active ingredients from Rhodiola rosea Tibetan traditional Chinese medicinal materials, and in particular relates to a method for preparing Rhodiola rosea solid-state transformation fermented product with microbial mixed strains, and the effective active ingredients in the fermented product are greatly improved. Background technique [0002] Rhodiola refers to the genus Rhodiola in the Sedum family. There are more than 90 species in the world, mostly distributed in the alpine regions of the northern hemisphere, and most of them grow under mountain flowstones or shrubs at an altitude of 3500-5000 meters. There are 73 species found in our country, which are mainly distributed in the alpine and alpine regions of Tibet. In addition, Rhodiola resources are also distributed in some areas of Northeast, Northwest, and North China. [0003] The main medicinal part of Rhodiola is the rhizome, a...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/685C12R1/69
Inventor 邓小晨
Owner SICHUAN UNIV