Method for cultivating regenerated plant from isolated microspore of brassica

A technology for microspore culture and plant regeneration, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problem of low reliability and stability of selected materials, low selection intensity, large population, etc. problems, to achieve the effect of accelerating the pace of breeding, enriching breeding resources, and wide adaptability

Inactive Publication Date: 2009-05-20
刘祥军
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Problems solved by technology

However, in order to obtain the parental material with a heterozygous genetic background, in many crops, artificial assisted self-pollination must be carried out, and it takes 6-10 years to achieve relative homozygosity. The strength should not be too

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1

[0014] 1) Free microspore culture:

[0015] Inoculate the microspores in the embryoid body induction medium at a concentration of 5×10 4 -5×10 5 Pcs / mL, transfer to 33℃, dark conditions and high temperature stress for 48 hours, then switch to 25℃, dark conditions to induce embryoid bodies;

[0016] 2) Differentiation culture:

[0017] After the torpedo stage to the cotyledon stage embryoid bodies are formed, the temperature is 24℃, the light is 2200LuX, and the photoperiod is 15hr / d. After 4-12 days, the embryoid bodies are inoculated into the differentiation medium. After turning green, transfer the embryos to MS hormone-free solid medium containing 3% sucrose to continue culture;

[0018] 3) Rooting culture:

[0019] After the stems and leaves are grown, the plants are transferred to a rooting medium, and the rooting culture is carried out under the conditions of 25°C, 2000 Lux, and 16 hours of light / day, until the seedlings of the free microspores regeneratin...

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Abstract

The invention provides a culture method for regenerated plantlets of Brassica isolated microspores, which relates to improvement of a culture technique for regenerated plantlets of Brassica isolated microspores as horticultural crops, and belongs to the field of biological breeding techniques. The invention provides the culture method applicable to regenerated microspore plantlets of Brassica crops. The culture method comprises the following steps: (1) culturing microspores; (2) performing differentiation culture; and (3) performing rooting culture. The culture method has the advantages of providing a large number of new genotype pure lines for breeding, greatly enriching breeding resources of Brassica, accelerating breeding pace, greatly shortening breeding time and having broad application prospects.

Description

technical field [0001] The invention relates to the improvement of the cultivation technology of the free microspore regeneration plant of the horticultural crop Brassica, and belongs to the technical field of biological breeding. Background technique [0002] Plants are heterosis, so current crop breeding focuses on cross breeding. In this way, on the one hand, the growth advantage of the hybrid can be used to ensure high yield and disease resistance of the crop variety. On the other hand, due to the complex genetic background of the hybrid, it is not suitable to save the seed for use, thereby protecting the interests of the hybrid developer. In crossbreeding, the genetic background of the parents used must be homozygous, and hybrids with the same traits can be obtained after mating. However, in order to obtain the parental material with a heterozygous genetic background, in many crops, artificial assisted self-pollination must be carried out, and it takes 6-10 years to ac...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 刘祥军
Owner 刘祥军
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