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Method for cultivating regenerated plant from isolated microspore of brassica

A technology for microspore culture and plant regeneration, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problem of low reliability and stability of selected materials, low selection intensity, large population, etc. problems, to achieve the effect of accelerating the pace of breeding, enriching breeding resources, and wide adaptability

Inactive Publication Date: 2009-05-20
刘祥军
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AI Technical Summary

Problems solved by technology

However, in order to obtain the parental material with a heterozygous genetic background, in many crops, artificial assisted self-pollination must be carried out, and it takes 6-10 years to achieve relative homozygosity. The strength should not be too high (to avoid the loss of good recessive traits), resulting in a large number of screened populations and low reliability and stability of the selected materials. Therefore, this traditional cross-breeding method is very time-consuming and laborious.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Free microspore culture:

[0015] Microspores were inoculated in embryoid body induction medium at a concentration of 5 × 10 4 -5×10 5 cells / mL, transferred to 33°C and dark for 48 hours, and then transferred to 25°C and dark to induce embryoid bodies;

[0016] 2) Differentiation culture:

[0017] After the embryoid body is formed from the torpedo stage to the cotyledon stage, transfer to the light conditions of temperature 24°C, light 2200LuX, and photoperiod 15hr / d to continue culturing for 4-12 days, and inoculate the embryoid body in the differentiation medium. After turning green, transfer the embryos to MS hormone-free solid medium containing 3% sucrose to continue culturing;

[0018] 3) Rooting culture:

[0019] After the stems and leaves grow, the plants are transferred into the rooting medium, and the rooting culture is carried out under the conditions of 25° C., 2000 Lux, and 16 hours of light / day until the seedlings of the regenerated plants from the f...

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PUM

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Abstract

The invention provides a culture method for regenerated plantlets of Brassica isolated microspores, which relates to improvement of a culture technique for regenerated plantlets of Brassica isolated microspores as horticultural crops, and belongs to the field of biological breeding techniques. The invention provides the culture method applicable to regenerated microspore plantlets of Brassica crops. The culture method comprises the following steps: (1) culturing microspores; (2) performing differentiation culture; and (3) performing rooting culture. The culture method has the advantages of providing a large number of new genotype pure lines for breeding, greatly enriching breeding resources of Brassica, accelerating breeding pace, greatly shortening breeding time and having broad application prospects.

Description

technical field [0001] The invention relates to the improvement of the cultivation technology of the free microspore regeneration plant of the horticultural crop Brassica, and belongs to the technical field of biological breeding. Background technique [0002] Plants are heterosis, so current crop breeding focuses on cross breeding. In this way, on the one hand, the growth advantage of the hybrid can be used to ensure high yield and disease resistance of the crop variety. On the other hand, due to the complex genetic background of the hybrid, it is not suitable to save the seed for use, thereby protecting the interests of the hybrid developer. In crossbreeding, the genetic background of the parents used must be homozygous, and hybrids with the same traits can be obtained after mating. However, in order to obtain the parental material with a heterozygous genetic background, in many crops, artificial assisted self-pollination must be carried out, and it takes 6-10 years to ac...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 刘祥军
Owner 刘祥军
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