Spirulina polysaccharide eye drop, and preparation method and application thereof
A technology of spirulina polysaccharide and eye drops, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., to achieve the effect of simple preparation method, easy implementation, and broad application and development prospects
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preparation example Construction
[0012] Step 1, preparation of spirulina polysaccharide: prepare the required spirulina polysaccharide of the present invention with the following process.
[0013] (1) Take the dry Spirulina platensis powder and add 95% ethanol, soak overnight; (2) Centrifuge and dry at 37°C, add double distilled water at a ratio of 1:12; (3) Adjust the pH to 10.0 with NaOH, and bathe in 80°C for 4 ~6h; (4) centrifuge, adjust pH to 7.0; (5) add 5% trichloroacetic acid for precipitation at 4°C overnight; (6) centrifuge, take supernatant, add 5% mass / volume Trichloroacetic acid precipitation for 3 hours; (7) Centrifuge, take the supernatant, add 5 times the volume of ethanol to precipitate, and overnight at 4°C; (8) Wash the precipitate twice with pure acetone, and vacuum freeze-dry; (9) Place the above precipitate in- Store at 20°C for more than 3 days to ensure the complete volatilization of acetone; (10) Add normal saline to fully dissolve the precipitate, centrifuge to remove undissolved sub...
Embodiment 1
[0018] First establish the animal model of alkali burn in C57 BL / 6 mice: the model animal is C57 BL / 66—8 weeks old, female mice, the mice are generally anesthetized, proparacaine hydrochloride eye drops are instilled in the eyes, and 3 μl 1N NaOH is directly dripped into the eyes. The surface of the mouse cornea was burnt with alkali for 30 seconds, and immediately rinsed with more than 20ml of normal saline.
[0019] The mouse alkali burn animal model established above was divided into two groups, the control group and the treatment group, and eye drops were applied immediately after burns. Each eye was given 5 μl of eye drops each time, 4 times a day, and the eye drops were treated for 7 days. The specific groups are as follows:
[0020] Control group: treated with Tiruo Compound Sodium Chloride Eye Drops.
[0021] Treatment group: Spirulina polysaccharide eye drops were used for eye drops.
[0022] After 7 days of treatment, the therapeutic effect was observed and compare...
Embodiment 2
[0024] When the polysaccharide concentration of the spirulina polysaccharide eye drops was 100 μg / ml, after 7 days, the corneal lesions of the mice were observed. It was found that the blood vessels in the control group mice grew significantly after alkali burns, and the growth area of new blood vessels accounted for 1 / 2 to 3 of the entire cornea. / 4, while the 10μg / ml spirulina polysaccharide eye drops treatment group only had a small amount of new blood vessels growing near the limbus, accounting for about 1 / 15 of the entire corneal area. The paraffin-embedded section of the whole eyeball was taken and stained with HE. It can be seen that the corneal stroma of the mice in the control group had obvious edema after 7 days of alkali burn, and a large number of inflammatory cells infiltrated. Adhesion: In the treatment group, no obvious edema was found in the corneal stroma of the mice after 7 days of alkali burn, only individual inflammatory cell infiltration was seen, and no ...
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