Long-acting composite contraception microspheres and preparation method thereof
A microsphere and compound technology, which is applied in the field of long-acting compound contraceptive microspheres and its preparation, can solve problems such as affecting liver and kidney functions, large side effects, and complicated use of long-acting contraceptive implants
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Embodiment 1
[0058] Example 1 Preparation of Gestodene / Ethinyl Estradiol Microspheres
[0059] Under room temperature conditions, weigh 250mg polylactic acid-glycolic acid (50:50, Mw=10000) and 5mg gestodene and 2mg ethinyl estradiol, dissolve with organic solvent 2ml dichloromethane, will contain PLGA and medicine The organic solution was quickly added to 60ml of 1% PVA solution, emulsified in an electric stirrer (1200rpm) for 10min, then poured into 160ml of 1%PVA solution, evaporated at 25°C for about 6 hours, and stood to separate the solidified PLGA microspheres , and freeze-dried after washing with double distilled water, the diameter of the microspheres is 40-80 microns.
[0060] The determination results of microsphere drug encapsulation efficiency are as follows: the encapsulation efficiencies of gestodene and ethinylestradiol were 77.61% and 69.38%, respectively.
Embodiment 2
[0061] Example 2 Preparation of Gestodene / Estradiol Microspheres
[0062] Under room temperature, weigh 250mg of polylactic acid (Mw=20000), 5mg of gestodene and 2mg of estradiol, dissolve it with 3ml of ethyl acetate as an organic solvent, and quickly add the organic solution containing polylactic acid and drugs to 100ml In 0.2% PVA solution, emulsify in electric stirrer (2000rpm) for 2min, then pour into 150ml 1%PVA solution, evaporate for about 6 hours at 25°C, stand to separate the solidified PLGA microspheres, and wash with distilled water After freeze-drying, the microspheres have a diameter of 20-60 microns.
[0063] The experimental results showed that the encapsulation efficiencies of gestodene and estradiol were 86.32% and 74.86%, respectively.
Embodiment 3
[0064] Example 3 Preparation of Gestodene / Ethinyl Estradiol Microspheres
[0065] At room temperature, weigh 250mg of polylactide-glycolide (75:25, Mw=10000), 5mg of gestodene and 2mg of ethinylestradiol, and dissolve them in 5ml of dichloromethane as an organic solvent. Add the drug organically to 200ml of 0.2% sodium carboxymethylcellulose solution, emulsify in an electric stirrer (1000rpm) for 5min, reduce the stirring speed to 300rpm, evaporate at 25°C for about 6 hours, and stand to separate the solidified PLGA The microspheres are obtained by washing with double distilled water and freeze-drying. The diameter of the microspheres is 60-120 microns.
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