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Magnesium (ion) diagnosis/measuring reagent kit and magnesium (ion) concentration determination method

A kit and ion technology, applied in the field of magnesium diagnosis/determination kits and determination of magnesium concentration, can solve problems such as calcium interference and interference

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is also calcium interference (within the physiological range) Ca 2+ Maximum interference 10% etc. insufficient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The magnesium (ion) diagnostic / measurement reagent in this embodiment is a single reagent, including:

[0051] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0052] Stabilizer 500mmol / L

[0053] Reduced coenzyme 0.25mmol / L

[0054] Hexokinase 6000U / L

[0055] Pyruvate carboxylase 8000U / L

[0056] Alanine dehydrogenase 10000U / L

[0057] Glucose 20mmol / L

[0058] Adenosine triphosphate 3mmol / L

[0059] Oxaloacetic acid 3mmol / L

[0060] Phosphate 3mmol / L

[0061] Ammonium chloride 3mmol / L

[0062] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0063] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the measured magnesium (ion) sample to the reagent is 1 / 25, the reaction direction...

Embodiment 2

[0066] The magnesium (ion) diagnostic / measurement reagent in this embodiment is a dual reagent, including:

[0067] Reagent 1

[0068] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0069] Stabilizer 50mmol / L

[0070] Reduced coenzyme 0.25mmol / L

[0071] Glucose 20mmol / L

[0072] Adenosine triphosphate 3mmol / L

[0073] Oxaloacetic acid 3mmol / L

[0074] Phosphate 3mmol / L

[0075] Ammonium chloride 3mmol / L

[0076] Reagent 2

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 500mmol / L

[0079] Hexokinase 6000U / L

[0080] Pyruvate carboxylase 8000U / L

[0081] Alanine dehydrogenase 10000U / L

[0082] After all the reagents are dissolved and prepared, they are divided into bottles to form a liquid double reagent, which can be used directly.

[0083] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, measu...

Embodiment 3

[0086] The magnesium (ion) diagnostic / measurement reagents in this embodiment are three reagents, including:

[0087] Reagent 1

[0088] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0089] Stabilizer 50mmol / L

[0090] Reduced coenzyme 0.25mmol / L

[0091] Glucose 20mmol / L

[0092] Adenosine triphosphate 3mmol / L

[0093] Oxaloacetic acid 3mmol / L

[0094] Phosphate 3mmol / L

[0095] Ammonium chloride 3mmol / L

[0096] Reagent 2

[0097] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0098] Stabilizer 500mmol / L

[0099] Pyruvate carboxylase 8000U / L

[0100] Alanine dehydrogenase 10000U / L

[0101] Reagent 3

[0102] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0103] Stabilizer 500mmol / L

[0104] Hexokinase 6000U / L

[0105] After all the reagents are dissolved and prepared, they are divided into bottles to form three liquid reagents, which can be used directly.

[0106] When determining the concentration of magnesium (...

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Abstract

The invention relates to a kit for diagnosing / measuring magnesium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of magnesium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glucose, adenyl pyrophosphate, oxaloacetic acid, phosphate radical, ammonium chloride, hexokinase, pyruvate carboxylase, lactamine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of magnesium (ions).

Description

Technical field [0001] The present invention relates to a magnesium (ion) diagnostic / determination kit. At the same time, the present invention also relates to a method for measuring the concentration of magnesium (ion), which belongs to the technical field of medical / food / environmental inspection and determination. Background technique [0002] There are many methods for magnesium determination, which can be summarized as colorimetry, fluorescence method, ion chromatography, ion selective electrode method (ISE), enzymatic method, atomic absorption spectrophotometry (AAS), isotope dilution mass spectrometry (ID-MS) Wait. [0003] The decisive method for magnesium determination is isotope dilution mass spectrometry, and the reference method is atomic absorption spectrophotometry. Although these methods have accurate results, the equipment is complicated and expensive, and they are not suitable for routine laboratories and automatic analysis. [0004] Colorimetric method is the mos...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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