Synthesis of (R)-styrene glycol by coupling acceleration of (R)-carbonyl reduction enzyme and formic dehydrogenase

Abstract

The invention relates to promotion of synthesis of (R)-styrene glycol by coupling (R)-carbonyl reductase and formic dehydrogenase, which belongs to the technical field of asymmetrical transformation of biological catalysis. The invention provides a recombinant Escherichia coli, namely E.coli Rosetta/ pETDuet-rcr-fdh, with a preservation number of CCTCC NO: M208123, wherein the (R)-special carbonyl reductasei and the formic dehydrogenase are coupled by a co-expression mode; asymmetrical transformation reaction is catalyzed by utilizing a cultured recombinant strain and taking 2-hydroxyacetophenone as a substrate; and the optical purity of the (R)-styrene glycol reaches 100 percent e.e., and the yield of the (R)-styrene glycol reaches 85.9 percent by optimizing the biotransformation reaction conditions. The invention utilizes double-enzyme coupling technology to solve the problem of limitation of regeneration cycle of coenzyme under the condition of high-concentration substrate, provides an effective path for high-efficiency and low-cost preparation of the (R)-styrene glycol, and lays a foundation for industrial application of the biosynthesized (R)-styrene glycol.

Description

technical field [0001] The invention relates to efficient preparation of (R)-phenylethylene glycol under high substrate concentration by using recombinant Escherichia coli co-expressed with (R)-carbonyl reductase gene and formate dehydrogenase gene, and belongs to the technical field of biocatalytic asymmetric transformation. Background technique [0002] Chiral compounds have been more and more widely used in the production of fine chemicals such as medicines, pesticides, hormones, and food additives. For example, optically pure phenylglycol is not only an indispensable and important chiral additive in liquid crystal materials, but also It is an important intermediate for the preparation of optically active medicines, pesticides and functional materials. [0003] Asymmetric reduction reactions catalyzed by oxidoreductases are often used in the preparation of chiral compounds. This type of reaction requires coenzymes as hydrogen or electron transmitters. Commonly used coenzy...

AI Technical Summary

Problems solved by technology

Coenzymes are generally unstable, expensive, and cannot be replaced by general synthetic substances

In the asymmetric reduction reaction of biocatalysis, the inability to recycle the coenzyme is the "bottleneck" factor that limits its industrial application

Whole cells can provide a certain amount of coenzyme for the reaction through their own metabolism, but according to the chemical equivalent balance, when the substrate concentration increases and the amount of coenzyme required for the reaction increases, the supply of coenzyme becomes the main limiting factor