Method of determining size of fatty acid content in intramuscular fat of cattle based on genotype of fatty acid synthase and method of determining eating quality of beef based on the result

Abstract

The object of the invention is to provide a method of determining a fatty acid composition contained in intramuscular fat based on the genotype of cattle, particularly a determination method for determining the size of oleic acid content simply and with high accuracy, and further provide a method of more objectively determining eating quality of beef based on the determination result. The invention provides a method of determining the size of fatty acid content in intramuscular fat of cattle based on the genotype of fatty acid synthase determined by an assay of the following and or the base of the and / or , and a method of determining whether or not beef with excellent eatingquality can be obtained from the cattle based on the result. A base of (either adenine (A) or guanine (G)) represents the base at the 16,024 position corresponding to a polymorphic site in a base sequence represented by SEQ ID NO: 1 in the sequence listing. A base of (either thymine (T) or cytosine (C)) represents the base at the 16,039 position corresponding to a polymorphic site in the base sequence.

Description

technical field [0001] The present invention relates to a method for judging whether it is beef or beef with good taste by judging the composition of fatty acids contained in muscle fat, especially the amount of oleic acid based on the genotype of fatty acid synthase (FASN) . Furthermore, it also relates to the method of selecting and breeding the cattle which can obtain beef with good taste based on the same judgment result. The present invention provides techniques that are particularly effective in the fields of animal husbandry (cattle breeding, breeding, breeding, improvement, etc.) and beef production and processing. Background technique [0002] Among the fatty acids contained in the intramuscular fat of beef, the monovalent unsaturated fatty acid (oleic acid) having 18 carbon atoms is the most contained, and in particular, it is reported that the meat of the Japanese breed of black hair is less oily than the meat of foreign breeds. The acid content is remarkably hi...

AI Technical Summary

Problems solved by technology

In addition, there are many problems that it is difficult to process a large number of samples in a short time because this measurement requires a lot of time, and since a large amount of organic solvent is used in the stage of fatty acid extraction, etc., it is impossible to ignore the adverse effects on the health of the tester. influence etc.

[0010] However, methods for determining fatty acid composition based on the genotype of fatty acid synthases are not known so far

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