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Method for preventing and treating virus disease of petunia with mild virus

A petunia and weak virus technology, applied in the field of plant virus prevention and control, can solve the problems of insufficiency, unfavorable industrialization, time-consuming and the like, and achieve the effects of improving work efficiency, improving quality, and producing large and colorful flowers.

Inactive Publication Date: 2009-08-05
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the methods of inoculation mostly adopt root soaking, spray gun and friction inoculation. Before inoculation of weak virus, seed disinfection and seedbed control of aphids and whitefly must be carried out to prevent seedlings from being infected by strong virus before inoculation of weak virus, which is time-consuming and expensive. The method is cumbersome, low efficiency and other shortcomings, so it is not conducive to industrialization and cannot meet the needs of modern agriculture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The method for the prevention and treatment of petunia virus disease by this weak virus is carried out as follows:

[0051] 1), the preparation of the culture medium, including each component of the basic culture medium and the culture medium of each stage of tissue culture and the weight per liter are:

[0052] (1) Basic medium: the basic medium adopts MS medium, wherein, agar 8g / L, pH5.8;

[0053] (2) Sterile seeding medium: 1 / 2MS+white sugar 20g / L;

[0054] (3) Induction medium: 1 / 3MS+BA 1.0mg / L and NAA 0.2mg / L+sucrose 30g / L;

[0055] (4) Proliferation medium: 1 / 2MS+BA 0.5mg / L and NAA 0.1mg / L+sugar 30g / L;

[0056] (5) Strong seedling medium: MS+BA 0.25mg / L and NAA 0.05mg / L+sugar 30g / L;

[0057] (6) Rooting medium: 1 / 2MS+NAA 0.05mg / L+white sugar 20g / L.

[0058] 2), virus-free cultivation of petunia shoot tip:

[0059] (1) Selection and sterilization of explants: take the seeds of petunia, inoculate them on the sterile sowing medium after sterilizing, cultivate them...

Embodiment 2

[0083] In this example, the agar of the basic medium is 7g / L, pH5.6; sterile seeding medium: 1 / 2MS+sugar 20g / L; induction medium: 1 / 3MS+BA 1.0mg / L and NAA 0.1mg / L L+sucrose 30g / L; proliferation medium: 1 / 2MS+BA 0.1mg / L and NAA 0.05mg / L+sugar 30g / L; strong seedling medium: MS+BA 0.1mg / L and NAA 0.01mg / L+sugar 30g / L; rooting medium: 1 / 2MS+NAA 0.01mg / L+sugar 20g / L.

[0084] Take the young shoots of petunia plants with pure character and strong growth in greenhouse potted plants, soak them in 75% alcohol for 0.5 minutes, then soak them in 0.1% mercuric chloride aqueous solution for 10 minutes, and finally wash them with sterile water for 3 to 5 times to kill them. Bacteria treatment, as explant material for shoot tip detoxification culture.

[0085] All the other steps and conditions are the same as in Example 1.

Embodiment 3

[0087] In this example, the agar of the basic medium is 9g / L, pH6.0; sterile seeding medium: 1 / 2MS+sugar 20g / L; induction medium: 1 / 3MS+BA 3.0mg / L and NAA 0.5mg / L L+sucrose 30g / L; proliferation medium: 1 / 2MS+BA 1.0mg / L and NAA 0.5mg / L+sugar 30g / L; strong seedling medium: MS+BA 0.5mg / L and NAA 0.1mg / L+sugar 30g / L; rooting medium: 1 / 2MS+NAA 0.1mg / L+sugar 20g / L.

[0088] Take the petunia seeds, soak them in 75% alcohol for 1.0 minutes, then soak them in 0.1% mercuric chloride aqueous solution for 30 minutes, and finally rinse them with sterile water for 3 to 5 times for sterilization, and use them as explants for the detoxification culture of stem tips. body material.

[0089] All the other steps and conditions are the same as in Example 1.

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Abstract

The invention relates to a method for preventing and controlling Petunia hybrida virus diseases through weak virus. The method is implemented according to the following steps of: 1) preparing a culture medium; 2) performing detoxification culture on the stem apexes of Petunia hybrida; 3) performing virus ELISA detection; 4) inoculating a plant with weak virus; and 5) performing tissue culture and propagation on the weak-virus plant. The method has the advantages that as a plant tissue culture technique is utilized to perform the tissue culture and propagation on the weak-virus plant, propagated tissue culture seedlings all carry the weak virus and can be continuously propagated as seeds for production, so that immunization which is required before production every year in the prior art is left out, and work efficiency is exponentially improved. The incidence of mosaic virus and similar diseases of the Petunia hybrida protected by the weak virus is lower than 3 percent, and the Petunia hybrida protected by the weak virus is healthy and strong in plant, large in flower and bright in color, so the quality of the Petunia hybrida protected by the weak virus is obviously improved.

Description

technical field [0001] The invention relates to the technical field of plant virus prevention and control, in particular to a method for attenuated virus prevention and treatment of petunia virus disease. Background technique [0002] Petunia (Petunia hybrida) is native to South America and belongs to the genus Petunia in the family Solanaceae. It is a herbaceous plant widely used in potted plants and flower beds. Because of its long flowering period and many varieties of flowers and colors, it is deeply loved by people, and it has high ornamental value in beautifying the environment. However, due to the serious harm of the virus, the affected plants have mottled leaves, forming mosaic leaves, with dark and light colors, often accompanied by curled leaves, weakened growth potential, deformed flowers, and smaller flowers, which greatly reduce their ornamental value. According to investigations, the incidence rate of seed propagation is 20-30%, and the incidence rate of asexu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00
Inventor 陈剑平徐刚汪一婷吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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