Purification method of penicillin and cephalosporin
A cephalosporin and purification method technology, applied in the field of chemical engineering, can solve the problems of cumbersome operation and long time required for separation, and achieve the effects of high separation efficiency, improved separation effect and long service life
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Embodiment 1
[0027] Weigh 3g of penicillin G sodium and dissolve it in water, and load it on the chromatographic column. After the Toyopearl HW-40F filler is fully swelled by the eluent (0.005mol / L, pH7.0 sodium citrate buffer solution), the column is packed by wet method. The column size is φ1.6cm×60cm, and the column volume (BV ) is 120.6 mL. At 5° C., 0.005 mol / L, pH 7.0 sodium citrate buffer was used to flow through the chromatographic column at an elution flow rate of 0.5 BV / hr, and the amount of eluent was 3 BV. Macromolecular impurities are eluted in the front, and penicillin G sodium is eluted in the back, and macromolecular impurities and penicillin G sodium are collected respectively and freeze-dried. The yield of penicillin G sodium was 95.8%.
[0028] The sensitization of macromolecular impurities and purified penicillin G sodium was tested by passive skin allergy test. Rabbit anti-penicillin-binding protein serum (preparation method as described in the Summary of the Invent...
Embodiment 2
[0030] Adjust the pH value of the 250 mg / mL penicillin G sodium solution to 8.0, place it at 25°C for 15 days, and load 2 mL of the sample on the chromatographic column. After the Toyopearl HW-40S filler is fully swelled by the eluent (0.1mol / L, pH7.0 sodium citrate buffer), the column is packed by wet method, the column size is φ1.6cm×33cm, and the column volume (BV ) is 66.4mL. At 25° C., 0.1 mol / L, pH 7.0 sodium citrate buffer was used to flow through the chromatographic column at an elution flow rate of 0.3 BV / hr, and the amount of eluent was 4 BV. Before the elution position of penicillin G sodium, ultraviolet absorbing substances were eluted and collected and freeze-dried. Passive skin sensitization test As described in Example 1, components eluting prior to penicillin G sodium can elicit an allergic reaction in guinea pigs.
Embodiment 3
[0032] Weigh 1 g of ampicillin, dissolve it in water, and load it on the chromatographic column. After the Toyopearl HW-40S filler is fully swollen by the eluent (0.5% sodium chloride solution), the column is packed by wet method. The column size is φ1.6cm×30cm, and the column volume (BV) is 60.3mL. At 15°C, 0.5% sodium chloride solution was used to flow through the chromatographic column at an elution flow rate of 0.5BV / hr, and the amount of eluent used was 3BV. Macromolecular impurities and ampicillin were collected separately and freeze-dried. The yield of ampicillin was 96.6%. Passive skin allergy test As described in Example 1, the macromolecular impurities in ampicillin cause allergic reactions in guinea pigs, and the purified ampicillin has no sensitization.
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