Method for producing xylitol and arabinose at the same time

A technology of arabinose and xylitol, which is applied in the biological field, can solve the problems of low product yield, potential safety hazards, and low separation efficiency, and achieve the effect of reducing production costs and improving resource utilization

Active Publication Date: 2009-08-05
唐传生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0031] The present invention provides a set of new methods for producing xylitol by fermenting hemicellulose hydrolyzate and extracting arabinose from hemicellulose hydrolyzate, which effectively solves the problem of producing xylitol by chemical process and the process of producing xylitol from hemicellulose In the process of extracting arabinose from cellulose hydrolyzate, due to the similar chemical properties between xylose and arabinose, the separation efficiency is low, the product yield is low, or the safety hazard caused by the use of organic solvents

Method used

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  • Method for producing xylitol and arabinose at the same time
  • Method for producing xylitol and arabinose at the same time
  • Method for producing xylitol and arabinose at the same time

Examples

Experimental program
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Effect test

Embodiment 1

[0065] 40kg corn husks, add 160L of water, boil. Filter off the liquid part, wash the residue once with water, add 80 L of 2% (w / w) sulfuric acid solution to the dried residue, and hydrolyze in a hydrolysis reactor at 125°C for 2 hours. Remove the residue by filtration, adjust the filtrate to pH 3 with calcium carbonate, remove the precipitate by filtration, add 1 kg of activated carbon to the filtrate, remove the activated carbon after adsorption treatment for 30 minutes, and then pass through the cation and anion exchange chromatography columns in turn to obtain a colorless and transparent syrup , and concentrated to the required concentration under reduced pressure. The ratio of glucose, xylose, and arabinose content in this syrup is approximately 1:2:1 (see image 3 ).

Embodiment 2

[0067] Add 280L of 2.4% (w / w) sulfuric acid solution to 40Kg of dried bagasse, and hydrolyze at 125°C for 2 hours. All the other follow-up processing steps are the same as in Example 1. The sugar syrup prepared from bagasse hemicellulose hydrolyzate has a content ratio of glucose, xylose and arabinose of about 1:10:1.

Embodiment 3

[0069] The corn bran hemicellulose hydrolyzate obtained in Example 1 was taken, and the hot water extract from 100 g of rice bran was added to each liter of fermentation broth to adjust the total sugar to 250 g / L, which contained about 120 g / L of xylose and arabinose 80g / L, sterilized at 110°C for 10 minutes. A 5L fermenter (Biotech-5BG; Shanghai Baoxin Bioengineering.Equipment, Shanghai, China) with a capacity of 3L was inoculated with fresh Candida tropicalis CCTCC M 205067 (Candida tropicalis CCTCC M 205067), with an inoculation volume of 5% (v / v), Ventilation volume 1vvm, stirring speed 300rpm, culture at 33°C for about 30h, xylose is converted into xylitol, and arabinose does not participate in the reaction ( Figure 4 ). After the fermentation was stopped, the cell pellet was collected by centrifugation, and the pelleted cells were adjusted into a slurry with fresh medium, and then injected back into the fermenter. The medium was topped up to the fill volume of the fir...

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Abstract

The invention relates to a method for directly producing xylitol and arabinose by hemicellulose hydrolysate. Raw materials rich in arabinoxylans are hydrolyzed by acid or enzyme to obtain hydrolysate rich in the arabinose and xylose; and the hydrolysate is inoculated into yeast strains which can ferment the xylose and produce the xylitol and can not utilize the arabinose after detoxification treatment. When the xylose concentration in fermentation liquid is lowered to a certain extend, the fermentation is stopped. Ion-exchange desalinization, discoloration and proper concentration are carried out on the fermentation liquid, and chromatographic resolution is carried out on the arabinose- and xylitol-containing solution on calcium positive ions to respectively obtain xylitol solution with the purity of 99 percent and multi-sugar solution taking the arabinose as main ingredient, from which crystal xylitol and crystal arabinose can be respectively obtained by treatment. The method combines the production processes of the xylitol and the arabinose, thereby reducing the production cost and increasing the use ratio of resources.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the process of using hemicellulose hydrolyzate rich in xylose and arabinose as a matrix, and fermented by a specific yeast strain, the main component xylose is selectively converted into xylitol, arabinose Without participating in the reaction, a fermentation broth containing almost no xylose but rich in xylitol and arabinose was obtained. After the cell-removed fermentation broth was purified by ion exchange, it was divided into two parts, the pure xylitol fraction and the arabinose-heterosaccharide fraction by the first chromatographic separation. The pure xylitol fraction is directly concentrated and crystallized to obtain crystalline xylitol. The arabinose-miscellaneous sugar fraction is separated by a second chromatography to remove most of the miscellaneous sugars. This purer arabinose fraction is then concentrated and crystallized. , to obtain crystalline arabinose. Background ...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12P7/18C12R1/74
Inventor 张厚瑞蔡爱华周玉恒覃香香陈海珊金科曾健智
Owner 唐传生物科技(厦门)有限公司
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