The invention discloses a dyeing method of a reactive dye with a high color fixation rate. The dyeing method comprises the following steps: S1, pretreatment: padding a texture to be dyed in a finishing solution containing chitosan quaternary ammonium salt, dipping for two times, rolling for two times, prebaking at the temperature of 80 to 100DEG C, baking at the temperature of 110 to 130DEG C, washing with cold water and then drying; S2, reactive dyeing: immersing into a dyeing solution of the reactive dye, dyeing at the temperature of 20 to 30DEG C, raising the temperature to be 50 to 70DEG C, then adding an organic solvent B, and raising the temperature to be 80 to 100DEG C; adding sodium carbonate according to the volume of the dyeing solution of the reactive dye, insulating heat and reducing to room temperature; S3, performing after treatment, water washing, soaping, water washing and drying in air. The dyeing method of the reactive dye with the high color fixation rate, provided by the invention, has the advantages of high color fixation rate and good level-dyeing property; in addition, the process is simple, and industrial implementation is easily realized; meanwhile, chemical substances with high price and greater toxicity are prevented from being used.