Immunochromatography test paper for fast diagnosis of human granulocytic anaplasmosis and its preparation

An immunochromatographic test strip and rapid diagnosis technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of inapplicability for rapid diagnosis, a large number of, labor-intensive, etc., and achieve economical benefits, high specificity and sensitivity, The effect of shortening the detection time

Inactive Publication Date: 2009-08-05
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The detection methods in the above-mentioned laboratories all require a large amount of rea

Method used

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  • Immunochromatography test paper for fast diagnosis of human granulocytic anaplasmosis and its preparation
  • Immunochromatography test paper for fast diagnosis of human granulocytic anaplasmosis and its preparation
  • Immunochromatography test paper for fast diagnosis of human granulocytic anaplasmosis and its preparation

Examples

Experimental program
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Embodiment 1

[0033] Embodiment 1, Figure 3 ~ Figure 4 An immunochromatographic test paper for rapidly diagnosing human granulocytic anaplasmosis is provided, and the preparation method selects the following main materials:

[0034] The self-made msp2 polyclonal antibody according to the routine method informed by "Molecular Cloning Book" was obtained by immunizing New Zealand white rabbits with recombinant expression of msp2 protein.

[0035] Goat anti-rabbit IgG antibody (Dingguo Company), chloroauric acid (Sigma Company), nitrocellulose (NC) membrane (Millipore Company), bovine serum albumin (BSA) (Sigma Company), polyethylene glycol (PEG) 20000 (Sigma Company), hydrolyzed casein (Sigma Company), and other commonly used reagents are analytical reagents.

[0036] The preparation method specifically carries out the following steps in sequence:

[0037] 1), preparation of colloidal gold pad 2 containing gold-labeled msp2 polyclonal antibody:

[0038] 1. Preparation of a colloidal gold s...

Embodiment 2

[0072] Embodiment 2, an immunochromatographic test paper for rapidly diagnosing human granulocytic anaplasmosis, its preparation method is:

[0073] In step 1) in ②: change the adjustment to pH 8.0 to adjust to pH 9.0; change the addition of 1 mg of msp2 polyclonal antibody to the addition of 0.8 mg of msp2 polyclonal antibody.

[0074] All the other are the same as in Example 1.

Embodiment 3

[0075] Embodiment 3, a kind of immunochromatographic test paper of rapid diagnosis human granulocytic anaplasmosis, its preparation method is:

[0076] In step 1) in ②: change the adjustment to pH 8.0 to adjust to pH 7.0; change the addition of 1 mg of msp2 polyclonal antibody to the addition of 1.2 mg of msp2 polyclonal antibody.

[0077] All the other are the same as in Example 1.

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Abstract

The invention discloses an immune chromatography reagent paper for rapidly diagnosing anaplasmosises of granular cells of a human body, comprising a sample loading pad (1), a colloidal gold pad (2) of an msp2 antibody containing a golden mark, a pyroxylin film (3) for wrapping a quality control line (6) and a detection line (5), and a sample absorbing pad (4), wherein the upper surface of the pyroxylin film (3) is respectively provided with the colloidal gold pad (2) and the sample absorbing pad (4); the sample loading pad (1) is arranged on the upper surface of the colloidal gold pad (2); the detection line (5) is an msp2 polyclonal antibody which is coated on the pyroxylin film (3); and the quality control line (6) is a goat-anti-rabbit IgG antibody which is coated on the pyroxylin film (3). The invention also provides a preparation method for the immune chromatography reagent paper which is suitable for diagnosing anaplasmosises of granular cells of a human body on site.

Description

technical field [0001] The invention belongs to the field of immunochromatographic test paper for rapid diagnosis, in particular to an immunochromatographic test paper containing gold-labeled msp2 antibody for detecting human granulocytic anaplasmosis and a preparation method thereof. Background technique [0002] Human granulocytic anaplasmosis (HGA) is caused by Anaplasma phagocytophilum (Anaplasma phagocytophilum) infecting neutrophils in human peripheral blood, mainly characterized by fever accompanied by leukopenia, thrombocytopenia, and multiple organ dysfunction Clinically manifested tick-borne disease. Since the first case of human granulocytic anaplasmosis was reported in the United States in 1994, about 600-800 cases have been reported in the United States every year in recent years. In 2006, a case of human granulocytic anaplasmosis was found in Anhui Province, and suspected cases also occurred in other provinces. At present, countries that have reported human g...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543G01N33/569
Inventor 张耀洲郑青亮舒建洪
Owner ZHEJIANG SCI-TECH UNIV
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