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Solid culture method of metarrhizium anisopliae

A technology of solid culture and solid medium, which is applied in the field of solid culture of Metarhizium anisopliae, can solve the problems of high humidity requirements, low production efficiency, and long fermentation cycle, so as to avoid early-stage pollution, high production efficiency, and fermentation short cycle effect

Inactive Publication Date: 2009-09-09
河北绿茵生化科技有限公司
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the solid culture of Metarhizium anisopliae at home and abroad generally adopts the frame type or plastic bag method for cultivation. In the cultivation process, not only the equipment is complicated, the pollution rate is high, and the humidity requirements are high, but also the fermentation period is long and the relative production efficiency is low.

Method used

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  • Solid culture method of metarrhizium anisopliae

Examples

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preparation example Construction

[0016] (1) The preparation of liquid spawn comprises the following steps:

[0017] Bacteria: Metarhizium anisopliae M105-32 (or other Metarhizium anisopliae can also be used), preserved by Hebei Institute of Biology, is a known public strain.

[0018] Incline strains: Subculture and activate Metarhizium anisopliae strains on test tube slopes at 25°C in advance, when the slopes are covered with green spores;

[0019] Liquid medium: Put 60ml of medium into each 250ml Erlenmeyer flask, steam sterilize at 115°C for 30 minutes;

[0020] Inoculation: Pick a small piece of bacterial lawn from the solid slope and insert it into the liquid shaker flask;

[0021] Cultivation: shake culture at 25±1℃ for 36-42 hours, grow into white viscous mycelium and inoculate it into solid medium.

[0022] (2) Solid culture:

[0023] a. Solid medium: wheat bran 60-90%, corn stalk powder 5-40% (the above two are 100); the following are the percentages of the above two: glucose 0.8-1.5g, ammonium sul...

Embodiment 1

[0027] (1) Preparation of liquid strain:

[0028] a. Incline strains: Subculture and activate the strains of Metarhizium anisopliae M105-32 with test tube slope medium at 25°C in advance, when the slope is covered with green spores;

[0029] b. Liquid medium: prepare 0.5L liquid medium, put 60ml medium into each 250ml Erlenmeyer flask, and steam sterilize at 115°C for 30 minutes;

[0030] c. Inoculation: Pick a small piece of bacteria lawn from the solid slope and put it into the liquid shaker bottle, and one slope can connect 6-8 bottles;

[0031] d. Cultivation: shake culture at 25±1°C for 36-42 hours, grow into white viscous mycelium and inoculate it into solid medium.

[0032] (2) Solid culture:

[0033] a. Solid medium: 4.2kg of wheat bran, 0.8kg of corn straw powder, 60g of glucose, 90g of ammonium sulfate, 55g of potassium dihydrogen phosphate, and 6L of tap water. Stir evenly in the solid medium, the pH is natural, divide into several small cloth bags, and put them ...

example 2

[0036] The solid slant medium has the following components and weight ratio: 80-120g potato, 16-24g sucrose, 4-6g yeast extract, KH 2 PO 4 1~4g, MgSO 4 1.2~1.8g, 15g agar, 1L water. (same as example 2)

[0037] The liquid culture medium used in the method of preparing liquid strains preferably has the following components and proportions: glucose 32-48g, yeast extract 4-6g, peptone 8-12g, KH 2 PO 4 3~8g, MgSO 4 0.4~0.6g, 1L of water. (same as example 2)

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Abstract

The invention relates to a solid culture method of metarrhizium anisopliae and the technical field of biological fermentation method. The method comprises preparation of liquid strain and solid culture. The prior period of the solid culture uses framework culture and the later period uses plastic bag culture. The solid culture medium includes nutritious raw material wheat bran and maize strain power as a raw material carrier. The beneficial effects of invention are that the combination of framework culture and plastic bag culture can effectively reduce pollution probability in the culture process and the requirements on humidity in the later period and obviously improve the ventilation performance of the culture medium; the fermentation technology is simple and does not need complex equipment, the fermentation period is short, the production efficiency is high, the production cost is low, and the economical benefit and social benefit are relatively high.

Description

technical field [0001] The invention relates to the technical field of biological fermentation methods, in particular to a solid culture method for Metarhizium anisopliae. Background technique [0002] Metarhizium anisopliae is a broad-spectrum parasitic fungus, which can infect more than 200 kinds of insects. It has special effects on locusts and underground pests. It has no residual toxicity, no pollution, is safe to use, and has a protective effect on natural enemies of pests. It is a sustainable and low-consumption pest control preparation, which can replace the use of a large number of chemical pesticides and reduce environmental pollution, and has important application value in biological control. [0003] The mass cultivation of Metarhizium anisopliae is the basis to meet the needs of field application. At present, the solid culture of Metarhizium anisopliae at home and abroad generally adopts the frame method or plastic bag method for cultivation. The cultivation pr...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 程辉彩张丽萍张根伟李书生史延茂董超黄亚丽段普凡崔冠慧尹淑丽
Owner 河北绿茵生化科技有限公司
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