Degerming method for recycling somatic cells from amino acid zymotic fluid/extraction waste liquid

A technology for bacterial cells and fermentation broth, applied in the field of fermented food industry, can solve the problems of inability to remove water-soluble proteins in fermentation broth, reduced protein content in filter residues, low bacterial cell removal rate, etc., and achieves low power consumption and reduced addition amount. , the effect of improving the filtration efficiency

Inactive Publication Date: 2009-09-30
JIANGNAN UNIV
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  • Abstract
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Problems solved by technology

However, simply adding flocculant, the removal rate of bacterial cells is low, and the water-soluble protein in the fermentation liquid or extraction waste liquid cannot be removed. In addition, the amount of flocculant is large, generally up to 300mg / L, and the treatment cost is high.
Moreover, the filtration after bacterial cell flocculation is still relatively difficult, and the moisture content of the filter residue is high, which not only increases the product loss, but also increases the energy consumption of the subsequent filter residue drying
[0008] For a long time, in order to speed up the filtration rate of flocculated bacterial cells, diatomite, sawdust, sugarcane pith and other filter aids were mainly added to the fermentation broth or extraction waste liquid, which achieved good results, but after adding filter aids , the protein content of the filter residue is reduced, and the potency as a feed is reduced

Method used

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  • Degerming method for recycling somatic cells from amino acid zymotic fluid/extraction waste liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: A method for removing bacteria from the amino acid fermentation liquid / extraction waste liquid, adopting the following process steps:

[0032] 1. Glutamic acid fermentation broth, glutamic acid concentration 12%, temperature 32°C, continuously sent to the tube side of the heat exchanger, the tube side of the heat exchanger is the glutamic acid from the maintenance tank at a temperature of 100 °C in step 3 For sour fermentation broth, the two materials exchange heat on the inner wall of the heat exchanger. After the heat exchange, the original 32°C fermentation broth is heated to 76°C, while the original 100°C fermentation broth is cooled to 50°C;

[0033] 2. The fermented liquid heated to 76°C flows into the heater, heated to 100°C with steam at a pressure of 0.3Mpa, then enters the maintenance tank, and stays in the maintenance tank for 30 minutes;

[0034] 3. Return the fermented liquid in the tank at 100°C to the tube side of the heat exchanger describe...

Embodiment 2

[0040] Embodiment 2: A method for removing bacteria from the amino acid fermentation liquid / extraction waste liquid, adopting the following process steps:

[0041] 1. Glutamic acid extraction waste liquid, glutamic acid concentration 2%, temperature 15 ℃, continuously sent to the shell side of the heat exchanger, the tube side of the heat exchanger is the valley of the temperature 80 ℃ from the maintenance tank in step 3 Acid extraction waste liquid, the two materials exchange heat in the heat exchanger, after the heat exchange, the original 15°C extraction waste liquid is heated to 45°C, while the original 80°C extraction waste liquid is cooled to 48°C;

[0042] 2. The extraction waste liquid heated to 45°C flows into the heater, heated to 80°C with steam at a pressure of 0.2Mpa, then enters the maintenance tank, and stays in the maintenance tank for 20 minutes;

[0043] 3. The extraction waste liquid in the maintenance tank at 80°C is refluxed to the tube side of the heat ex...

Embodiment 3

[0049] Embodiment 3: A method for removing bacteria from the amino acid fermentation liquid / extraction waste liquid, adopting the following process steps:

[0050] 1. Lysine fermentation broth, lysine concentration 16%, temperature 30°C, continuously sent to the shell side of the heat exchanger, the tube side of the heat exchanger is lysine from the maintenance tank at a temperature of 80°C in step 3 For acid fermentation broth, the two materials exchange heat in the heat exchanger. After the heat exchange, the extraction waste liquid at 30°C is heated to 50°C, while the lysine fermentation liquid at 80°C is cooled to 53°C;

[0051] 2. The lysine fermentation broth heated to 50°C flows into the heater, heated to 80°C with steam at a pressure of 0.3Mpa, then enters the maintenance tank, and stays in the maintenance tank for 25 minutes;

[0052] 3. The lysine fermentation liquid in the maintenance tank at 80°C is returned to the tube side of the heat exchanger described in step ...

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Abstract

The invention relates to a degerming method for recycling somatic cells from amino acid zymotic fluid / extraction waste liquid. The method is characterized by comprising the following steps: the amino acid zymotic fluid or the extraction waste liquid flows into a heat exchanger to exchange heat with zymotic fluid or extraction waste liquid from a maintenance tank; the zymotic fluid or the extraction waste liquid after heat exchange flows into a heater to heat and then exchanges heat with low-temperature raw liquid for cooling; the amino acid zymotic fluid or the extraction waste liquid after the heat exchange enters a storage tank and is injected into an ejector; air is sucked in and beaten into small bubbles by a pump impeller; and afterwards, a flocculating agent is added to a mixer so that the somatic cells are agglomerated into groups which enter an air-bearing tank after flocculation; the flocculent groups absorbing the small bubbles have low specific gravity, automatically float upwards to the top of the tank and overflow to a somatic cell collecting tank; clear zymotic fluid or extraction waste liquid after degerming flows out from one side of the air-bearing tank; the somatic cells overflowing to the collecting tank are filtered by a filter, and obtained clear liquid and clear liquid flowing out of the air-bearing tank are mixed.

Description

technical field [0001] The invention relates to a method for removing bacteria from amino acid fermentation liquid / extraction waste liquid, specifically, it mainly refers to recovering bacteria from amino acid fermentation liquid such as glutamic acid and lysine or waste liquid after amino acid extraction. A somatic cell belongs to the technical field of fermented food industry. Background technique [0002] my country is a major producer of amino acids. In 2007, the domestic annual output of glutamic acid reached 1.5 million tons and lysine 750,000 tons. In addition, there are a large number of small varieties of amino acids such as threonine, tryptophan, and arginine. In various amino acid fermentation liquids, generally 1.0-10.0% of bacterial cells are contained, which brings certain difficulties to the extraction work, resulting in a decline in the extraction yield and purity of the product. Removing bacterial cells from the fermentation broth can not only create good c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02
Inventor 毛忠贵张建华
Owner JIANGNAN UNIV
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