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Medicament extracting device and method from body fluid example

A technology for biological fluids and extraction devices, which is applied in the preparation of test samples, measurement devices, chemical instruments and methods, etc., can solve the problem of affecting sensitivity, difficult to achieve liquid-liquid extraction or solid-phase extraction, and difficult to achieve high-throughput automated operation. And other issues

Active Publication Date: 2009-10-07
天津博纳艾杰尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein precipitation method has the characteristics of simplicity and low cost, but there are many residual interfering substances, especially fatty interfering substances are difficult to remove, thus affecting the sensitivity of detection
The liquid / liquid extraction method, especially the use of secondary reverse extraction, can obtain better purification effect of the sample; but this method is not easy to realize high-throughput automatic operation, which limits the work efficiency
Solid-phase extraction can usually achieve better purification results and is suitable for high-throughput automation; however, method development is more complicated and reproducibility is poor
On the other hand, a large number of strongly water-soluble drugs or their metabolites are difficult to achieve regardless of liquid-liquid extraction or solid-phase extraction due to their strong polarity and strong water solubility.

Method used

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  • Medicament extracting device and method from body fluid example
  • Medicament extracting device and method from body fluid example

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A method for extracting medicines in biological fluid samples, comprising the steps of:

[0064] 1. Add the sample into the extraction device; the adsorption material filled in the tubular column of the extraction device includes: styrene-divinylbenzene copolymer containing sulfonic acid groups and styrene-divinylbenzene copolymer, the weight of the two The ratio is 1:9, the particle size of the adsorption material is 5 microns, and the pore size is 60

[0065] 2. Add methanol and let it stand for 1 minute; the volume ratio of sample, adsorption material and methanol is 1:1:10;

[0066] 3. By pressurizing the inlet end of the tubular cylinder of the extraction device, the solution flows into the inlet end, passes through the adsorption material, and then flows out from the outlet end at a flow rate of 0.2 mL per minute. The effluent is used for further analysis and detection.

Embodiment 2

[0068] A method for extracting medicines in biological fluid samples, comprising the steps of:

[0069] 1. Add the sample to the extraction device; the adsorption material filled in the tubular column of the extraction device includes: styrene-divinylbenzene copolymer containing carboxylic acid groups and alkane substitution and alkane-substituted styrene-divinylbenzene copolymer matter, the weight ratio of the two is 2:8, the particle size of the adsorption material is 30 microns, and the pore size is 100

[0070] 2. Add ethanol and let it stand for 2 minutes; the volume ratio of sample, adsorption material and ethanol is 3:1:12;

[0071] 3. By applying vacuum from the outlet end of the tubular cylinder, the solution flows into the inlet end, passes through the adsorption material, and flows out from the outlet end at a flow rate of 0.6mL per minute, and the effluent is taken for further analysis and detection.

Embodiment 3

[0073] A method for extracting medicines in biological fluid samples, comprising the steps of:

[0074] 1. Add the sample to the extraction device; the adsorption materials filled in the tubular column of the extraction device include: styrene-divinylbenzene copolymers containing phosphoric acid groups and lipid groups and styrene-divinylbenzene copolymers containing amide groups Benzene copolymer, the weight ratio of the two is 3:7, the particle size of the adsorption material is 60 microns, and the pore size is 150

[0075] 2. Add acetonitrile and let it stand for 3 minutes; the volume ratio of sample, adsorption material and acetonitrile is 5:1:10;

[0076] 3. Through its own gravity, the solution flows into the inlet port, and flows out from the outlet port after passing through the adsorption material. The flow rate is 1mL per minute, and the effluent is taken for further analysis and detection.

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Abstract

The invention relates to a medicament extracting device and a method from body fluid example, wherein the device is a tubular cylinder with an entry end and an exit end; the device has two sieve plates, and an exit sieve plate and an entry sieve plate are fixed at two ends of the adsorption material in the cylinder respectively; the adsorption material filled in the tubular cylinder is ion exchange resin or a mixture of ion exchange resin and lipophilic reverse adsorption material. The medicament extracting method from body fluid example comprises: adding the example into the extracting device; adding organic solvent or mixing solution of the organic solvent and water and stewing; making example and the extracting solution flowing out from the exit end by pressurization, vacuum pumping or self gravity. The medicament extracting device and the method can remove main interference substrate like protein, polypeptide, amino acid and fatty from the biological sample, and hold measured medicaments in the example solution, thereby achieving purposes of purification and enrichment.

Description

technical field [0001] The invention relates to a device and method for extracting drugs from biological fluid samples. The device and method for extracting drugs from biological fluid samples can remove main interfering substrates such as proteins, polypeptides, amino acids and fats in biological samples, and extract the tested The drug remains in the sample solution to achieve the purpose of sample purification and enrichment. Background technique [0002] The analysis of drug components and concentrations contained in biological samples (serum, plasma, urine and other animal body fluids) is an important content in pharmacokinetic and drug metabolism studies. Currently used analytical methods mainly rely on liquid chromatography coupled with multistage mass spectrometry. Before using the liquid chromatography-mass spectrometry method for analysis, it is necessary to purify the relevant biological samples to remove related substances such as proteins, peptides and fats in ...

Claims

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Application Information

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IPC IPC(8): B01D15/08B01J20/281B01J20/287B01J39/26B01J41/20G01N1/34G01N30/06
Inventor 汪群杰黄韦朱旭东张俊燕许平
Owner 天津博纳艾杰尔科技有限公司
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