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Method for preparing cyanide-urea triamide hapten, artificial antigen and antibody as well as application thereof

A technology of cyanuric triamide and artificial antigen, which is applied in chemical instruments and methods, receptors/cell surface antigens/cell surface determinants, testing dairy products, etc., to achieve the effect of safety monitoring

Inactive Publication Date: 2009-10-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no related technical reports on the preparation of cyanuric triamide hapten, antigen and antibody

Method used

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  • Method for preparing cyanide-urea triamide hapten, artificial antigen and antibody as well as application thereof
  • Method for preparing cyanide-urea triamide hapten, artificial antigen and antibody as well as application thereof
  • Method for preparing cyanide-urea triamide hapten, artificial antigen and antibody as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Hapten 1 Synthesis

[0041] With 6-aminocaproic acid containing 6 carbon atoms as raw material, 5.4mmol of 2,4-diamino-6-chloro-1,3,5-triazine and 0.9mmol of 6-aminocaproic acid were packed into a circle In the bottom flask, add 100mL acetone, then add 2.7mmoL potassium hydroxide, react at 40°C for 48h, evaporate the solvent under reduced pressure to obtain a concentrate, dissolve the concentrate in 25mL of 10% aqueous sodium bicarbonate solution, and wash twice with n-hexane , and then the aqueous phase was acidified to pH 2 with concentrated acetic acid, and the precipitate was collected to obtain the hapten (CAAT-AC6).

[0042] Hapten identification results:

[0043] MS(EI), m / z(%): 240(M + , 11.68), 181 (50.88), 167 (17.28), 153 (39.01), 139 (100), 126 (31.09). to C 9 h 16 o 2 N 6 Carry out HRMS, the theoretical value is 2401329, and the measured value is 240.1329. IR(KBr)v max / cm -1 : 3492(s), 3409(s), 3331(s), 3159(s), 2937(s), 2860(w), 1642(v...

Embodiment 2

[0044] Example 2 Synthesis of Hapten 2

[0045] Take the aminopropionic acid containing 3 carbon atoms as a raw material example: put 5.4mmol of 2,4-diamino-6-chloro-1,3,5-triazine and 5.4mmol of aminopropionic acid into a round bottom flask , add 100mL ethanol, then add 10.8mmoL triethylamine, react at 70°C for 9h, evaporate the solvent under reduced pressure to obtain a concentrate, dissolve the concentrate in 25mL of 5% aqueous sodium bicarbonate solution, wash twice with dichloromethane, and then The aqueous phase was acidified with hydrochloric acid to a pH value of 1, and the precipitate was collected to obtain the hapten 3-(4,6-diamino-1,3,5-triazine-2-amino)-propionic acid (English name: 3-( 4,6-diamino-1,3,5-triazin-2-ylthio) propanoic acid), named M-a-3-BSA.

Embodiment 3

[0046] Example 3 Hapten 3 Synthesis

[0047] With 3-mercaptopropionic acid containing 3 carbon atoms as raw material, 5.4mmol of 2,4-diamino-6-chloro-1,3,5-triazine and 1.8mmol of 3-mercaptopropionic acid were loaded into the circle In the bottom flask, add 100mL tetrahydrofuran, then add 4.5mmoL potassium hydroxide, react at 60°C for 38h, evaporate the solvent under reduced pressure to obtain a concentrate, dissolve the concentrate in 25mL 6% aqueous sodium bicarbonate solution, and wash twice with petroleum ether , and then the aqueous phase was acidified with hydrochloric acid to a pH value of 2, and the precipitate was collected to obtain a hapten.

[0048] When amino acids or mercapto acids with carbon chains of other lengths are used as raw materials, the synthesis method is the same as in Examples 1-3.

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Abstract

The invention discloses a method for preparing cyanide-urea triamide hapten, artificial antigen and antibody as well as application thereof. The method takes 2, 4-diamino-6-chlorine-1, 3, 5-triazine as raw material which reacts with amino acid or mercaptan acid under the alkaline condition to generate the hapten containing carboxyl; then, the hapten containing carboxyl is coupled with protein by a method of active ester or mixed acid anhydride to prepare the artificial antigen. After animals are immunized by the artificial antigen, cyanide-urea triamide specific antibody can be obtained. The antibody can be used for the immunodetection of cyanide-urea triamide, so that the safe monitoring of the cyanide-urea triamide can be realized.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to the preparation method and application of the core reagent hapten, artificial antigen and antibody of the rapid detection technology of cyanuric triamide. Background technique [0002] Cyanuron triamide, also known as melamine, 1,3,5-triazine-2,4,6-triamine, 2,4,6-trisemicarbazide, is the manufacture of cyanuron triamide-formaldehyde resin (melamine Plastic) raw materials, often used in the manufacture of daily utensils, decorative veneers, fabric finishing agents, etc. In China, the most common application of cyanuric triamide-formaldehyde resin (ie, melamine plastic) in daily life is a type of plastic dishes called "melamine dishes (also known as melamine bowls)". The physical properties of this type of vessel are very similar to ceramics, hard and non-deformable but not as brittle as ceramics. It is often marked with a warning "do not use in microwave oven...

Claims

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Application Information

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IPC IPC(8): C07D251/70C07D251/52C07K14/765C07K14/77C07K14/80C07K14/42C07K14/705C07K14/47C07K16/44C12P21/08G01N33/577G01N33/02G01N33/04G01N33/18
Inventor 雷红涛孙远明宋利军沈玉栋杨金易张挺王弘徐晓艳柳春红
Owner SOUTH CHINA AGRI UNIV
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