Immunity bead chromatograph test strip for quick testing ractopamine and preparation method thereof

A technology of immunomagnetic bead chromatography and ractopamine, which is applied to measurement devices, analytical materials, instruments, etc., to achieve the effects of short reaction time, cheap instruments and equipment, and high sensitivity

Inactive Publication Date: 2009-10-21
JIANGNAN UNIV
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  • Description
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There is no report on the development of rapid diagnostic test strip

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  • Immunity bead chromatograph test strip for quick testing ractopamine and preparation method thereof
  • Immunity bead chromatograph test strip for quick testing ractopamine and preparation method thereof
  • Immunity bead chromatograph test strip for quick testing ractopamine and preparation method thereof

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preparation example Construction

[0027]2) Preparation and purification of polyclonal antibody: RAC-BSA was used as the immunogen, and New Zealand white rabbits were used as the immunized animals. The immune source was mixed with an equal amount of Freund's complete adjuvant to make an emulsifier during the first immunization. Multi-point subcutaneous injection on the back of white rabbits, the immunization dose is 1 mg / rat. After 2-4 weeks, the same dose of immune source and the same amount of Freund's incomplete adjuvant are mixed to make an emulsifier, and the immunization is strengthened. During the immunization, the antibody effect is monitored. Valence and specificity, the last immunization without adjuvant. Seven days after the last immunization, the heart was bled, and the purified RAC polyclonal antibody was obtained by fractional precipitation with sulfuric acid. And the antibody was purified by ammonium sulfate precipitation method; rabbit anti-RAC-BSA IgG was obtained.

[0028] 3) Preparation of i...

Embodiment 1

[0033] Weigh 2g of growing and finishing pig compound feed sample into a 50mL centrifuge tube, add 6mL of acetonitrile extract, shake it by hand several times, then add 6mL of carbonate buffer solution, and then add 10mL of ethyl acetate, shake and extract for 30min, Then ultrasonically extract for 10 min, and finally centrifuge at a low speed of 3000 rpm for 10 min, transfer the upper organic phase to another new test tube, and dry it with nitrogen gas. Add 50 μL of methanol to dissolve the dried residue, and dilute with carbonate PBS buffer to obtain the sample solution to be tested. Finally, use the ractopamine immunomagnetic bead chromatography test strip to detect the sample solution. The insertion depth does not exceed the marked line. The sample will flow through the test strip through capillary chromatography. After 10 to 20 seconds, take out the test strip and store it at room temperature. After 15 minutes of reaction, put the test strip into the Enviso TM The Magne...

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Abstract

The invention relates to an immunity bead chromatograph test strip for quick testing ractopamine and a preparation method thereof, belonging to the technical field of ractopamine test. The invention is composed of a scale board, a sample pad, a magnetic combination pad, a coated film, an absorbent pad and the sample pad; the magnetic combination pad, the coated film and the absorbent pad are connected one by one on the scale board. The magnetic combination pad is made from absorbed glass mat which is used to absorb ractopamine magnetic antibodies; detection line T which is printed by the carrier protein solution coupled with ractopamine and control line C which is printed by goat anti-rabbit IgG are positioned on the coated film. The preparation method comprises the following steps: inserting the sample pad of the test paper in the sample solution to be tested, taking out the sample pad after 10-20 seconds, reacting at room temperature for 15 minutes, testing the test paper in a magnetic signal detector which outputs the biological response signals converted to magnetic field signals in the form of electrical signals; drawing standard curve and then evaluating the ractopamine content in the sample to be tested according to the standard curve. The invention applies the nano-magnetic immunity technology to test ractopamine and has the characteristics of high sensitivity, short reaction time, cheap apparatus, simple usage and the like.

Description

technical field [0001] A nano-immune magnetic bead chromatography test strip for rapidly detecting ractopamine and a preparation method thereof relate to the technical field of ractopamine detection. Background technique [0002] Ractopamine (RAC) is a phenamine adrenaline hormone. RAC is a phenylethanolamine derivative with molecular formula C 18 h 23 o 3 N, molecular weight 301, chemical name is 1-(4-hydroxyphenyl)-2-[1-methyl-3-(4-hydroxyphenyl)-propylamino]-ethanol hydrochloride, molecular agonist, can Strengthen heart contraction, expand skeletal muscle blood vessels and bronchial smooth muscle, and can be used to treat shock and bronchospasm in veterinary medicine and medicine. In recent years, with the in-depth research on RAC, it has been shown that RAC has the functions of strengthening metabolism in vivo, promoting fat decomposition, protein synthesis, reducing fat production and reducing protein decomposition, so the energy and components of synthetic fat can ...

Claims

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Application Information

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IPC IPC(8): G01N33/566G01N33/532G01N33/558
Inventor 胥传来李灼坤刘丽强马伟
Owner JIANGNAN UNIV
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