Preparation methods of sustained-release microcapsule and sustained-release composite membrane for inhibiting monilinia fructicola

A brown rot fungus and composite film technology, applied in botany equipment and methods, chemicals for biological control, fungicides, etc., can solve the problems of banning drug resistance of bacteria, prolonging drug effect time, economic losses, etc.

Inactive Publication Date: 2009-11-11
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Peach brown rot is a fungal disease that damages the flowers, leaves, branches and fruits of peach trees, especially the fruits, causing a large number of fruit drop and rot, causing huge economic losses
Traditional chemical pesticides are gradually banned due to environmental pollution and bacterial resistance
At present, there are no natural pesticides that can effectively control...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] Preparation of slow-release microcapsules inhibiting peach brown rot:

[0019] 1) Weigh chitosan and berberine, the mass ratio of chitosan and berberine is 9-11: 0.22-0.28, add acetic acid solution, after chitosan and berberine fully swell, stir to dissolve, Stand still, remove bubbles, obtain berberine-chitosan solution, standby;

[0020] 2) Add the berberine-chitosan solution dropwise into soybean oil containing Span 80, stir at a constant temperature of 40-50°C, and emulsify for 28-32 minutes to form a W / O system;

[0021] 3) Add glutaraldehyde solution dropwise, stir for 2.5-3.5 hours, and centrifuge to make the microcapsules settle completely;

[0022] 4) Pour out the upper preparation solution, wash the obtained microcapsules with petroleum ether for 3-5 times, and vacuum-dry the obtained microcapsules at 48-52° C. for 23-25 ​​hours to obtain the final product.

[0023] Microcapsule slow-release and antibacterial function: use pH6.8 phosphate buffer (take 250 mL...

Embodiment 1

[0028] Weigh chitosan 9g and berberine 0.22g, add 0.18wt% acetic acid solution 9mL and wait for it to fully swell, stir to make it dissolve, let stand, remove air bubbles, obtain berberine-chitosan solution, set aside; Add the berberine chitosan solution dropwise to 68mL soybean oil containing 0.18wt% Span80, stir at a constant temperature of 40°C, and emulsify for 28min to form a W / O system; add 1.1mL 48wt% glutaraldehyde solution dropwise , stirred for 2.5 hours, and centrifuged to make the microcapsules settle completely; pour the upper preparation solution, wash the obtained microcapsules with petroleum ether for 3 times, and vacuum-dry the obtained microcapsules at 48°C for 23 hours to obtain the final product.

[0029] The results showed that the initial release rate of the drug was fast, reaching 16% in the first day, and then the release rate slowed down. The accumulative release rate of berberine reached 72% in one month, and the inhibitory rate of the drug to pathogen...

Embodiment 2

[0031] Weigh chitosan 10g and berberine 0.25g, add 0.18wt% acetic acid solution 9mL and wait for it to fully swell, stir to make it dissolve, let stand, remove air bubbles, obtain berberine-chitosan solution, and set aside; Add berberine chitosan solution dropwise into 68mL soybean oil containing 0.18wt% Span80, stir at a constant temperature of 45°C, and emulsify for 30min to form a W / O system; add 1.1mL 48wt% glutaraldehyde solution dropwise , stirred for 3 hours, and centrifuged to make the microcapsules settle completely; pour the upper layer preparation solution, wash the obtained microcapsules with petroleum ether for 3 times, and vacuum-dry the obtained microcapsules at 50°C for 24 hours to obtain the final product.

[0032] The results showed that the initial release rate of the drug was fast, reaching 17% in the first day, and then the release rate slowed down. The accumulative release rate of berberine reached 76% in one month, and the inhibitory rate of the drug to p...

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Abstract

The invention discloses preparation methods of a sustained-release microcapsule and a sustained-release composite membrane for inhibiting the monilinia fructicola. The preparation method of the sustained-release microcapsule comprises the following steps: the chitosan and the berberine are weighed, the mass ratio between the chitosan and the berberine is 9-11:0.22-0.28, the acetate solution is added, then the chitosan and the berberine are sufficiently swelled to obtain berberine-chitosan solution after stirring for dissolving, standing and air bubble removal, and the berberine-chitosan solution is added in the soybean oil containing span 80, stirred at the constant temperature of 40-50 DEG C and then emulsified for 28-32 min to form a W/O system; the glutaraldehyde solution is added for stirring for 2.5-3.5 hours, and the centrifuging is carried out so that the microcapsule is fully settled; and the preparation liquid on the upper layer is obliquely taken, the obtained microcapsule is washed with petroleum ether for 3-5 times and then dried for 23-25 hours under the vacuum at the temperature of 48-52 DEG C. The invention also provides the preparation method for the sustained-release composite membrane for inhibiting the monilinia fructicola. Both the microcapsule and the composite membrane have the sustained-release effects and have no toxic side effect to the environment and the plants since the berberine is a native compound.

Description

technical field [0001] The invention belongs to the field of fungicide production, and relates to a method for preparing slow-release microcapsules and a composite film of berberine (also known as berberine) extracted from traditional Chinese medicine on peach brown rot fungus (Monilinia fructicota). Background technique [0002] Screening antifungal active substances from medicinal plants is an important direction in the research and development of fungicides. my country has rich resources of medicinal plants, drawing on the research results of traditional Chinese medicine, it is expected to screen out natural active substances against phytopathogenic fungi from medicinal plants. These active substances can be directly used as natural fungicides, or used as lead compounds to develop highly active fungicides through structural modification. [0003] Peach brown rot is a fungal disease that damages the flowers, leaves, branches and fruits of peach trees, especially the fruit...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01N43/16A01N25/28A01N25/34A01P3/00
Inventor 田平芳侯东耀阎春琦葛喜珍
Owner BEIJING UNIV OF CHEM TECH
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