Novel azulene compounds and application thereof
A compound and drug technology, applied in the field of medicine, can solve problems such as scarcity, limited application research, single type, etc., and achieve a strong effect of removing free radicals
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] At room temperature, put 300g (wet weight) mycelia in a Erlenmeyer flask, extract with 800ml 80% acetone, separate the extract by silica gel column chromatography, and use chloroform and methanol as the mobile phase for elution (100:1, 100:3, 100:5, 100:8, 100:10, 9:1, 7:1, 5:1, 3:1), take the 100:5 part. The 100:5 part was depigmented with Sephadex LH-20, the mobile phase was 1:1 volume ratio of chloroform and methanol, and the golden yellow one was taken for later use. The part prepared by the above method is roughly separated by medium and low pressure, and the 70% and 80% parts are combined, and then analyzed and prepared by liquid phase (methanol: water = 70%), the elution time is 40 minutes and 44 minutes respectively. Two compounds, compound I (3.2 mg) and compound II (4.1 mg), were obtained in minutes, and the purity reached above 98% after analysis.
Embodiment 2
[0024] At room temperature, put 300g (wet weight) of mycelia in a Erlenmeyer flask, extract with 800ml of 100% acetone, and separate the extract by silica gel column chromatography, eluting with chloroform and methanol as the mobile phase, taking 20:1 part. The 20:1 part was depigmented with LH-20, and the volume ratio of chloroform and methanol was 1:1 as the mobile phase, and the lighter color was taken for later use. The part prepared by the above method was cut roughly under medium and low pressure, and then analyzed and prepared in liquid phase (methanol: water = 70%). The elution time was 40 minutes and 44 minutes respectively, and the two compounds were obtained. The purity of compound Ⅰ (2.2 mg) and compound Ⅱ (3.3 mg) reached over 98% after analysis.
Embodiment 3
[0026] Precisely pipette a small volume of the sample solution into a 5mL volumetric flask, add 3mL of 31.52μg / mL DPPH solution precisely to each, and dilute to volume with methanol. After standing in the dark at room temperature for 1 h, the absorbance was measured at 516 nm.
[0027] The formula for calculating the clearance rate of DPPH: clearance rate (%)=[1-(DPPH·) t , / (DPPH ) t=0 ]×100%
[0028] where (DPPH·) t=0 is the initial concentration of DPPH free radicals in the system; (DPPH·) t is the concentration of DPPH free radicals in the aftertreatment solution for 1 h. The sample (μg) / DPPH (μg) was plotted against the DPPH free radical scavenging rate.
[0029] As the dose increases, the clearance rate gradually increases until it reaches a relatively stable level, and the change trend of the clearance rate tends to be gentle.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More