Method for constructing diabetes mellitus animal model

Abstract

The invention discloses a method for constructing a diabetes mellitus animal model, which comprises the following steps: an alloxan reagent is injected in the abdominal cavity of a selected Kunming white mouse; then the white mouse is fed in a cage independently; water and food are fed to the white mouse quantitatively; after the white mouse is forbidden to eat food for 12 hours, blood is taken from the tail of the white mouse; a blood sugar meter is used for detecting a blood sugar value, and when the blood sugar value is larger than or equal to 10.0 mmol / L, the steps are ended. Proved by a statistical analysis of a blood sugar measuring result of the diabetes mellitus animal model, the method for constructing a white mouse diabetes mellitus model by utilizing the alloxan is feasible, and the model can be used as a research model of the pathogenesis and the drug mechanism of diabetes mellitus. Moreover, compared with the prior art, the constructed while mouse diabetes mellitus model has the advantages of simple model constructing method, high model forming rate, low cost, short period, good reproducibility, and the like.

Description

technical field [0001] The invention relates to a method for constructing a diabetes animal model, in particular to a method for constructing a mouse diabetes model. Background technique [0002] Animal models of human diseases are an extremely important experimental method and means in modern medical research. Using animal models of human diseases to study human diseases can carry out various experiments that are not easy to see and inconvenient to conduct experiments on patients. The study of human disease. At the same time, it can also overcome the interference of factors such as slow occurrence and development of human diseases, long incubation period, various causes of disease, and often accompanied by various other diseases. At present, the commonly used animal models of human diseases include experimental animal models and spontaneous animal models. Spontaneous animal models have high application value, but the cost of modeling is high, and the conditions for feeding...

AI Technical Summary

Problems solved by technology

At present, the commonly used animal models of human diseases include experimental animal models and spontaneous animal models. Spontaneous animal models have high application value, but the cost of modeling is high, and the conditions for feeding and breeding are strict, so they cannot be widely used; while experimental animal models are generally The modeling is simple and easy, the molding rate is high and the cost is low, and it is widely used at home and abroad

[0003] The existing experimental animal models of diabetes mainly have the following construction methods: 1) Surgical modeling method. The diabetes model produced by this method is relatively stable, but for small animals, the operation is more complicated, and duodenum may occur after the operation. Complications such as necrosis are only applicable to a small number of animals and do not have universal application value; 2) Virus-induced modeling method, this method has certain development prospects for the construction of type I diabetes models, but the modeling rate is not high, and there are some side effects. At present, it is rarely used; 3) STZ modeling method, this method can be injected with a large dose or multiple small doses, the model is stable, simple and easy to implement, and is more commonly used, but this method has the disadvantages of long modeling cycle and high cost; 4 ) alloxan modeling method. The animal currently selected for this method is rats. Although the rat diabetes model constructed by this method has the advantages of simple operation, stable blood sugar level and long-term maintenance, it is a commonly used method for evaluating the efficacy and safety of diabetes drugs. Models are widely used, but the mortality rate of modeling is high, the modeling cycle is longer and the cost is higher