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Method for preparing agar sugar

A kind of technology of agarose and agarose, which is applied in the field of preparation of agarose, can solve the problems of restricting the industrial production of enterprises, the inability to produce agarose, and high production costs, so as to improve the economic benefits of enterprises and resolve foreign technology monopoly , the effect of low production cost

Inactive Publication Date: 2009-12-16
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There have been a few literature reports on the preparation of agarose at home and abroad, but its production cost is high, the yield is low, or it is in a technical secret. The reported technology basically cannot produce agarose that meets the quality, which limits the industrialization of enterprises. Production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of the agarose provided by the present embodiment comprises the following steps:

[0030] (1) Ethanol treatment of agar powder: Weigh 10 g of agar powder, add 200 mL of ethanol solution with a volume concentration of 60%, soak at room temperature for 3 hours, stir once every 30 minutes, after soaking, filter and wash with deionized water to remove ethanol ;

[0031] (2) Cellulose pretreatment: weigh 20g of DE-52 cellulose, add 200mL of deionized water to soak overnight, and then preheat the overnight cellulose solution to 85°C with a water bath;

[0032] (3) Cellulose adsorption: add 400 mL of deionized water to the agar gel treated in step (1), boil and dissolve on an electric stove, adjust the temperature to 85°C, and mix it with the pretreated cellulose solution in step (2), Stir in a water bath at a constant temperature of 85°C for 2 hours;

[0033] (4) Filtration, gel: the agar and cellulose mixed solution obtained in step (3) are filtered ...

Embodiment 2

[0036] The preparation method of the agarose provided by the present embodiment comprises the following steps:

[0037] (1) Ethanol treatment of agar strips: Weigh 10g of agar strips, add them to 600mL ethanol solution with a volume concentration of 60%, soak at room temperature for 3-4 hours, stir once every 30 minutes, after soaking, filter and wash with deionized water to ethanol;

[0038] (2) Cellulose pretreatment: weigh 20g of DE-52 cellulose, add 200mL of deionized water to soak overnight, and then preheat the overnight cellulose solution to 80-85°C with a water bath;

[0039] (3) Cellulose adsorption: add 400mL of deionized water to the agar strip treated in step (1), boil and dissolve on an electric stove, adjust the temperature to 80-85°C, and mix with the pretreated cellulose in step (2) Mix the solutions and stir in a water bath at a constant temperature of 80-85°C for 2-3 hours;

[0040] (4) Filtration, gel: the mixed solution of agar and cellulose obtained in s...

Embodiment 3

[0043] The preparation method of the agarose provided by the present embodiment comprises the following steps:

[0044] (1) Agar strip bleaching: Weigh 5g of agar strip (light yellow), put it into 400mL potassium permanganate solution with a pH of 4.0 and a mass concentration of 0.05g / mL, and after bleaching for 10min, stir in the bleaching process for 2-3 The next time, discard the bleach solution, wash with water until neutral, and then add 400mL of oxalic acid (H 2 C 2 o 4 ) solution, soak for 10 minutes, stir 2 to 3 times during soaking, wash with water until neutral, and rinse with deionized water to remove residual ions;

[0045] (2) Agar gel ethanol treatment: put the agar gel strips processed in step (1) into 400 mL of ethanol solution with a volume concentration of 60%, soak for 4 hours at room temperature, stir every 1 hour, after soaking, filter and use Repeated immersion in deionized water to remove ethanol;

[0046] (3) Cellulose pretreatment: Weigh 10g of DE-...

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Abstract

The invention relates to a method for preparing agar sugar, comprising the following steps: (1) ethanol treatment: adding ethanol solution with a volume concentration of 60% to the agar, soaking the mixture for 3-4h at room temperature, filtering the mixture and washing away the ethanol by de-ionized water, adding the de-ionized water according to the weight measurement ratio of 1:40 between the agar and the de-ionized water and heating and dissolve the mixture to prepare the agar solution; (2) cellulose adsorption: taking the agar solution obtained in step (1) and adjusting temperature thereof to 80-85 DEG C, mixing the agar solution with the pretreated cellulose solution, stirring the mixture at constant temperature for 2-3h in water bath; (3) filtration, gelation and dehydration and drying: filtrating the mixed solution of agar and cellulose, then collecting filtrate, carrying out cooling and gelation, freezing the obtained gel to ice at minus 18 DEG C; after dehydration, drying, crashing and sifting out the gel, thus obtaining the agar sugar; the preparation method features easy operation, low production cost, stable production quality and the capacity of improving economic benefits of manufacturing enterprises.

Description

technical field [0001] The invention relates to a preparation method of agarose, which belongs to the technical field of algae deep processing. Background technique [0002] Agarose is a processed product of red algae. It is the best quality product in the tertiary structure agar series and has a wide range of uses in medicine. The appearance of agarose is pure white powder, which can be dissolved in hot water and form a gel after condensation. It is used as a support for gel electrophoresis and an affinity chromatography carrier. , important reagents for biochemical analysis, protein, nucleic acid, antigen, antibody, and can be used for polysaccharide separation, purification and pharmaceutical preparation. [0003] At present, the annual demand for agarose in the international market is more than 30 tons, while the world's annual production is less than 20 tons, and the gap between supply and demand is more than 10 tons. Due to the existence of a gap between supply and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 杨贤庆戚勃李来好赵永强刁石强陈胜军岑剑伟石红郝淑贤马海霞
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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